Results 81 to 90 of about 3,711,584 (360)

An image processing pipeline for electron cryo‐tomography in RELION‐5

bioRxiv
Electron tomography of frozen, hydrated samples allows structure determination of macromolecular complexes that are embedded in complex environments.
Alister Burt, Bogdan Toader, Rangana Warshamanage, Andriko von Kügelgen, Euan Pyle, J. Zivanov, Dari Kimanius, T. Bharat, S. Scheres   +8 more
semanticscholar   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Hands‐on protocol for preparing water‐soluble fractions from agri‐food samples for NMR‐based metabolomics analysis

FEBS Open Bio, EarlyView.
This research protocol outlines a workflow for nuclear magnetic resonance (NMR)‐based metabolomics in the agri‐food sector. Using two case studies—strawberry leaves (solid matrix) and wine (liquid matrix)—it details the procedures for sample preparation, data acquisition, and processing.
Andrea Fernández‐Veloso, Jaime Hiniesta‐Valero, Alejandra Guerra‐Castellano, Miguel A. De la Rosa, Irene Díaz‐Moreno   +4 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Metformin promotes mitochondrial integrity through AMPK‐signaling in Leber's hereditary optic neuropathy

FEBS Open Bio, EarlyView.
Metformin mediates mitochondrial quality control in Leber's hereditary optic neuropathy (LHON) fibroblasts carrying mtDNA mutations. At therapeutic levels, metformin activates AMPK signaling to restore mitochondrial dynamics by promoting fusion and restraining fission, while preserving mitochondrial mass, enhancing autophagy/mitophagy and biogenesis ...
Chatnapa Panusatid, Rapasviranda Soiyangsuk, Maneeluck Tanadjindarat, Chayanon Peerapittayamongkol   +3 more
wiley   +1 more source

Simulation on Line C of the China–Central Asia Gas Pipeline based on SPS software

You-qi chuyun, 2020
The Line C of the China–Central Asia Gas Pipeline is a typical gas pipeline characterized by high transmission pressure, large flow, long pipeline span, numerous gas stations and complicated transportation technology.
Yansong LI, Mei ZHANG, Baoying WANG, Xinxing JIANG, Jianfei SUN, Yongtu LIANG   +5 more
doaj   +1 more source

A Method for Assessing Risk Rating of Natural Gas Pipeline Based on Accident Statistics [PDF]

Journal of Risk Analysis and Crisis Response (JRACR), 2012
This article collected 199 cases of domestic natural gas pipeline leakage accidents, and used the mathematical methods and SPSS statistical analysis software to analysis these accident cases.
Xiaoyuan Xu, Xuanya Liu, Ge Guo, Chao Ji
doaj   +1 more source

MethylCoder: software pipeline for bisulfite-treated sequences [PDF]

Bioinformatics, 2011
Abstract Motivation: MethylCoder is a software program that generates per-base methylation data given a set of bisulfite-treated reads. It provides the option to use either of two existing short-read aligners, each with different strengths. It accounts for soft-masked alignments and overlapping paired-end reads.
Brent, Pedersen, Tzung-Fu, Hsieh, Christian, Ibarra, Robert L, Fischer   +3 more
openaire   +2 more sources

TreeViewer: Flexible, modular software to visualise and manipulate phylogenetic trees

Ecology and Evolution
Phylogenetic trees illustrate evolutionary relationships between taxa or genes. Tree figures are crucial when presenting results and data, and by creating clear and effective plots, researchers can describe many kinds of evolutionary patterns.
G. Bianchini, P. Sánchez-Baracaldo
semanticscholar   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

FEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold, Janik Riese, Lancelot Seillier, Mark Kühnel, Julia Pannhausen, Charlotte O. J. Fröhlich, Christian Martin, Peter Boor, Matthias Saar, Danny D. Jonigk, Nadine T. Gaisa, Michael Rose   +11 more
wiley   +1 more source