Results 121 to 130 of about 282,600 (283)

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Editorial: Plant specialized metabolism for plant protection: Genomics and biotechnology

Frontiers in Plant Science, 2022
Zhihua Liao, Zhihua Liao, Lei Zhang, Felipe Vázquez-Flota   +3 more
doaj   +1 more source

The use of maize haploidy inducers as a tool in agricultural plant biotechnology. [PDF]

Vavilovskii Zhurnal Genet Selektsii, 2022
Ulyanov AV, Karlov AV, Khatefov EB.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Iron homeostasis disruption and lipid peroxidation in skeletal muscle during short‐term immobilization

FEBS Open Bio, EarlyView.
14‐day casting‐induced immobilization reduced gastrocnemius muscle mass and increased non‐heme iron and ferritin heavy chain levels. Despite iron accumulation, transferrin receptor 1 and iron regulatory protein 2 were paradoxically upregulated. Lipid peroxidation was elevated without compensatory antioxidant responses.
Haruka Yokogawa, Kazuhiko Higashida, Naoya Nakai   +2 more
wiley   +1 more source

PUBLIC APPROVAL OF PLANT AND ANIMAL BIOTECHNOLOGY IN KOREA: AN ORDERED PROBIT ANALYSIS

This study analyzes predictors of Korean public acceptance of the use of biotechnology to create genetically modified food products. Results indicate that the consumers with above average knowledge of specific outcomes of genetic modification were more ...
Onyango, Benjamin M., Jang, Ho-Min, Puduri, Venkata S., Hallman, William K., Govindasamy, Ramu   +4 more
core   +1 more source

Optimization of CRISPR-Cas9 system in Eustoma grandiflorum

iScience
Summary: The optimization of the CRISPR-Cas9 system for enhancing editing efficiency holds significant value in scientific research. In this study, we optimized single guide RNA and Cas9 promoters of the CRISPR-Cas9 vector and established an efficient ...
Xueqi Li, Fanqi Bu, Lishan Wang, Cholmin Kim, Wanjie Xue, Man Zhang, Saneyuki Kawabata, Qingzhu Zhang, Yuhua Li, Yang Zhang   +9 more
doaj   +1 more source

Improved the Activity of Phosphite Dehydrogenase and its Application in Plant Biotechnology. [PDF]

Front Bioeng Biotechnol, 2021
Liu T, Yuan L, Deng S, Zhang X, Cai H, Ding G, Xu F, Shi L, Wu G, Wang C.   +9 more
europepmc   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Fostering innovation in a small open economy: The case of the New Zealand biotechnology sector

, 2000
The New Zealand Biotechnology sector is worthy of study for several reasons. While there is a large and growing international literature on economic aspects of biotechnology innovation these studies concentrate on the United States and Europe.
Marsh, Dan
core