Results 51 to 60 of about 29,509 (216)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

The Bamboo Flowering Cycle Sheds Light on Flowering Diversity

Frontiers in Plant Science, 2020
Bamboo is a perennial flowering plant with a distinctive life cycle: many bamboo species remain in the vegetative phase for decades, followed by mass synchronous flowering and subsequent death.
Xiao Zheng, Xiao Zheng, Xiao Zheng, Shuyan Lin, Shuyan Lin, Huajun Fu, Huajun Fu, Huajun Fu, Yawen Wan, Yawen Wan, Yawen Wan, Yulong Ding, Yulong Ding   +12 more
doaj   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Potential Pathway of Nitrous Oxide Formation in Plants

Frontiers in Plant Science, 2020
Plants can produce and emit nitrous oxide (N2O), a potent greenhouse gas, into the atmosphere, and several field-based studies have concluded that this gas is emitted at substantial amounts.
Arbindra Timilsina, Arbindra Timilsina, Chuang Zhang, Chuang Zhang, Bikram Pandey, Bikram Pandey, Fiston Bizimana, Fiston Bizimana, Wenxu Dong, Chunsheng Hu, Chunsheng Hu   +10 more
doaj   +1 more source

Acute caffeine treatment protects the developing retina from ischemia‐induced cell death

FEBS Open Bio, EarlyView.
Caffeine reduces cell death in the developing retina under ischemia (OGD). This effect does not involve BDNF upregulation or antioxidant pathways (NRF2/VEGF). Neuroprotection occurs mainly through adenosine A2A receptor antagonism, decreasing glutamate release and excitotoxicity, highlighting caffeine's potential as an acute neuroprotective agent in ...
Amanda Alves Nascimento, Danniel Pereira‐Figueiredo, Gabriella Grossi de Lima Dias, Gabriel Ferreira dos Santos, Mariana Rodrigues Pereira, Rafael Brito, Karin C Calaza   +6 more
wiley   +1 more source

Impacts of Sand Burial and Wind Erosion on Regeneration and Growth of a Desert Clonal Shrub

Frontiers in Plant Science, 2018
Sand burial and wind erosion caused by sand movement are common phenomena in desert environments, but the effects on clonal shrub have rarely been investigated.
Baoli Fan, Baoli Fan, Changming Zhao, Xiaowei Zhang, Kun Sun   +4 more
doaj   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Hyperactive ice‐binding proteins stabilize cell membranes and improve resistance to dehydration stress in Caenorhabditis elegans

FEBS Open Bio, EarlyView.
TisIBP8, a fungal‐derived hyperactive ice‐binding protein, helps Caenorhabditis elegans survive dehydration. It localizes near cell membranes, reduces cell damage, and helps maintain membrane structure during drying. These results suggest that ice‐binding proteins can protect cells from dehydration stress as well as freezing stress.
Daiki Shimose, Kotaro Ozaki, Ryohei Kuriyama, Yuka Ikemoto, Kazuhiro Mio, Yuji C. Sasaki, Tatsuya Arai, Sakae Tsuda, Yoichi Shinkai, Masahiro Kuramochi   +9 more
wiley   +1 more source

Short-term evaluation of oxygen transfer from rice (Oryza sativa) to mixed planted drought-adapted upland crops under hydroponic culture

Plant Production Science, 2017
Mixed cropping is a cultivation method widely practiced in tropical regions. The newly developed close mixed planting technique mitigates the flood stress of drought-adapted upland cereal species by co-growing rice (Oryza sativa) plants under field flood
Morio Iijima, Yoshihiro Hirooka, Yoshimasa Kawato, Yoshinori Watanabe, Kaede C. Wada, Nodoka Shinohara, Pamwenafye I. Nanhapo, Maliata A. Wanga, Koji Yamane   +8 more
doaj   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source