Characterisation of a mobilisable plasmid conferring florfenicol and chloramphenicol resistance in Actinobacillus pleuropneumoniae [PDF]
The complete nucleotide sequence of a 7.7 kb mobilisable plasmid (pM3446F), isolated from a florfenicol resistant isolate of Actinobacillus pleuropneumoniae, showed extended similarity to plasmids found in other members of the Pasteurellaceae containing ...
Bossé+24 more
core +3 more sources
Step by Step Design of Popular and Common Vectors in Genetic Manipulation Using CRISPR/Cas9 System
Background and Aim: Genome editing is an efficient and accurate method in biological and medical studies. Among the wide range of genome editing techniques, clustered regularly interspaced short palindromic repeats (CRISPR) is one of the simplest and yet
MINA KOLAHDOUZMOHAMMADI+4 more
doaj
IncHI2 ST3 plasmids are known carriers of multiple antimicrobial resistance genes. Complete plasmid sequences from multiple drug resistant Escherichia coli circulating in Australian swine is however limited.
Ethan R. Wyrsch+7 more
doaj +1 more source
Comparative genomic analysis of two novel plasmids from Acidithiobacillus ferrivorans strain PQ33
Acidithiobacillus ferrivorans is a psychrotolerant acidophile capable of growing and oxidizing ferrous and sulphide substrates at low temperatures. To date, six genomes of this organism have been characterized; however, evidence of a plasmid in this ...
Robert Ccorahua+6 more
doaj +1 more source
Supercoiling Enhances DNA Mobility by Reducing Threadings and Entanglements [PDF]
DNA is increasingly employed for bio and nanotechnology thanks to its exquisite versatility and designability. Most of its use is limited to linearised and torsionally relaxed DNA but non-trivial architectures and torsionally constrained -- or supercoiled -- DNA plasmids are largely neglected; this is partly due to the limited understanding of how ...
arxiv
Detection of Plasmid-Mediated AmpC β-Lactamase Genes in Clinical Isolates by Using Multiplex PCR
Therapeutic options for infections caused by gram-negative organisms expressing plasmid-mediated AmpC β-lactamases are limited because these organisms are usually resistant to all the β-lactam antibiotics, except for cefepime, cefpirome, and the ...
F. Pérez-Pérez, N. Hanson
semanticscholar +1 more source
Tumor microenvironment drives cancer formation and progression. We analyzed the role of human cancer‐associated adipocytes from patients with renal cell carcinoma (RCC) stratified as lean, overweight, or obese. RNA‐seq demonstrated that, among the most altered genes involved in the tumor–stroma crosstalk, are ADAM12 and CYP1B1, which were proven to be ...
Sepehr Torabinejad+13 more
wiley +1 more source
\u3cem\u3eRhizobium phaseoli\u3c/em\u3e Symbiotic Mutants with Transposon Tn5 Insertions [PDF]
Rhizobium phaseoli CFN42 DNA was mutated by random insertion of Tn5 from suicide plasmid pJB4JI to obtain independently arising strains that were defective in symbiosis with Phaseolus vulgaris but grew normally outside the plant.
Cevallos, Miguel A.+4 more
core +1 more source
Plasmid DNA damage induced by singlet molecular oxygen released from the naphthalene endoperoxide DHPNO2 and photoactivated methylene blue [PDF]
To investigate oxidative lesions and strand breaks induction by singlet molecular oxygen (1O2), supercoiled-DNA plasmid was treated with thermo-dissociated DHPNO2 and photoactivated-methylene blue.
Baptista, Maurício da Silva+5 more
core +1 more source
Fitness Advantage of mcr-1–Bearing IncI2 and IncX4 Plasmids in Vitro
The objective of this study was to assess the impact of diverse plasmids bearing colistin resistance gene mcr-1 on host fitness. Forty-seven commensal E.
Renjie Wu+8 more
doaj +1 more source