Results 121 to 130 of about 980,548 (309)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Plasmid R1 is present as clusters in the cells of Escherichia coli

, 2000
Fluorescence microscopy was used to determine the location(s) of the replication origin of plasmid R1 in exponentially growing cells of Escherichia coli.
Nordstrom, K, Dasgupta, S, Tao, WT,
core  

Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel

Journal of Infection in Developing Countries, 2007
Background: Antibiotic resistance is common among bacterial pathogens associated with both community acquired and nosocomial infections. In view of the present problem of drug resistance we investigated the prevalence of methicillin resistant ...
Asad U Khan,1 Ayesha Sultan,1 Anju Tyagi,2 Shazia Zahoor,3 Mohd Akram,1 Sukhminderjit Kaur,3 Mohd Shahid,2 Chetana V Vaishnavi1
doaj  

Decoding the enigma: unveiling the transmission characteristics of waterfowl-associated blaNDM-5-positive Escherichia coli in select regions of China

Frontiers in Microbiology
Escherichia coli (E. coli) serves as a critical indicator microorganism for assessing the prevalence and dissemination of antibiotic resistance, notably harboring various antibiotic-resistant genes (ARGs).
Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Yanxi Shu, Zhechen Yang, Zhijun Zhong, Mingshu Wang, Mingshu Wang, Mingshu Wang, Mingshu Wang, Renyong Jia, Renyong Jia, Renyong Jia, Renyong Jia, Shun Chen, Shun Chen, Shun Chen, Shun Chen, Mafeng Liu, Mafeng Liu, Mafeng Liu, Mafeng Liu, Dekang Zhu, Dekang Zhu, Dekang Zhu, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Ying Wu, Ying Wu, Ying Wu, Ying Wu, Qiao Yang, Qiao Yang, Qiao Yang, Qiao Yang, Juan Huang, Juan Huang, Juan Huang, Juan Huang, Xumin Ou, Xumin Ou, Xumin Ou, Xumin Ou, Di Sun, Di Sun, Di Sun, Di Sun, Bin Tian, Bin Tian, Bin Tian, Bin Tian, Zhen Wu, Zhen Wu, Zhen Wu, Zhen Wu, Yu He, Yu He, Yu He, Yu He, Anchun Cheng, Anchun Cheng, Anchun Cheng, Anchun Cheng   +65 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Complete Sequence of Plasmid pLH1 from Lactobacillus helveticus ATCC15009: Analysis Reveals the Presence of Regions Homologous to Other Native Plasmids from the Host Strain

, 1999
The complete sequence for plasmid pLH1 from Lactobacillus helveticus ATCC15009 has beendetermined. Analysis of the 19,360-bp primary sequence revealed a putative replication origin andinitiation protein, information that could provide the basis for the ...
McConville, K J, Thompson, J K, Nicholson, C, Collins, M A, Pridmore, R D, Foley, Sophie   +5 more
core   +1 more source

Real-time plasmid transmission detection pipeline

Microbiology Spectrum
The spread of antimicrobial resistance among bacteria by horizontal plasmid transmissions poses a major challenge for clinical microbiology. Here, we evaluate a new real-time plasmid transmission detection pipeline implemented in the SeqSphere+ (Ridom ...
Natalie Scherff, Jörg Rothgänger, Thomas Weniger, Alexander Mellmann, Dag Harmsen   +4 more
doaj   +1 more source

The C‐terminal region of KIF26B is indispensable for nephron progenitor condensation and kidney formation in mice

FEBS Open Bio, EarlyView.
KIF26B plays an important role in kidney development. We engineered mice lacking the C‐terminal region of KIF26B and found severe kidney defects, including bilateral renal agenesis, similar to full Kif26b knockout mice. The mutation disrupted nephron progenitor condensation and reduced Gdnf‐Wnt11 signaling, showing that the KIF26B C‐terminal region is ...
Yuta Yamamura, Kengo Furuichi, Hiromitsu Akamatsu, Naoki Yamamoto, Sako Keisuke, Akihiko Koshino, Taichiro Minami, Megumi Oshima, Akinori Hara, Norihiko Sakai, Miho Shimizu, Kenta Takahashi, Minako Yamamura, Shin‐ichi Horike, Takiko Daikoku, Kenichi Harada, Yohei Shinmyo, Hiroshi Kawasaki, Takashi Wada, Yasunori Iwata   +19 more
wiley   +1 more source

Dissemination of IncQ1 Plasmids Harboring NTE_KPC-IId in a Brazilian Hospital

Microorganisms
KPC is a clinically significant serine carbapenemase in most countries, and its rapid spread threatens global public health. blaKPC transmission is commonly mediated by Tn4401 transposons.
Camila Maria dos Santos Boralli, Julian Andres Paganini, Rodrigo Silva Meneses, Camila Pacheco Silveira Martins da Mata, Edna Marilea Meireles Leite, Anita C. Schürch, Fernanda L. Paganelli, Rob J. L. Willems, Ilana L. B. C. Camargo   +8 more
doaj   +1 more source