Results 131 to 140 of about 1,239,164 (310)

The cooperative regulation of miR‐221 by APE1 and AUF1 impacts p27Kip1 defining a miR signature relevant for cervical cancer

FEBS Open Bio, EarlyView.
A regulatory axis involving APE1, AUF1, and miR‐221 is proposed. Pri‐miR‐221 is processed by DROSHA and DICER to generate mature miR‐221, which targets p27Kip1 mRNA. APE1 and AUF1 compete for pre‐miR‐221 binding. Reduced APE1/AUF1 levels impair miR‐221 biogenesis, decrease p27Kip1 mRNA degradation, and promote cell cycle progression, chemoresistance ...
Matilde Clarissa Malfatti, Nicolò Gualandi, Gianluca Tell, Giulia Antoniali   +3 more
wiley   +1 more source

Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control

FEBS Open Bio, EarlyView.
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada, Mina Iwaki, Kyosuke Hagiri, Rei Izumi, Masahiko Harata, Chihiro Horigome   +5 more
wiley   +1 more source

Amplification of mecA gene in multi-drug resistant Staphylococcus aureus strains from hospital personnel

Journal of Infection in Developing Countries, 2007
Background: Antibiotic resistance is common among bacterial pathogens associated with both community acquired and nosocomial infections. In view of the present problem of drug resistance we investigated the prevalence of methicillin resistant ...
Asad U Khan,1 Ayesha Sultan,1 Anju Tyagi,2 Shazia Zahoor,3 Mohd Akram,1 Sukhminderjit Kaur,3 Mohd Shahid,2 Chetana V Vaishnavi1
doaj  

Decoding the enigma: unveiling the transmission characteristics of waterfowl-associated blaNDM-5-positive Escherichia coli in select regions of China

Frontiers in Microbiology
Escherichia coli (E. coli) serves as a critical indicator microorganism for assessing the prevalence and dissemination of antibiotic resistance, notably harboring various antibiotic-resistant genes (ARGs).
Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Shaqiu Zhang, Yanxi Shu, Zhechen Yang, Zhijun Zhong, Mingshu Wang, Mingshu Wang, Mingshu Wang, Mingshu Wang, Renyong Jia, Renyong Jia, Renyong Jia, Renyong Jia, Shun Chen, Shun Chen, Shun Chen, Shun Chen, Mafeng Liu, Mafeng Liu, Mafeng Liu, Mafeng Liu, Dekang Zhu, Dekang Zhu, Dekang Zhu, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Xinxin Zhao, Ying Wu, Ying Wu, Ying Wu, Ying Wu, Qiao Yang, Qiao Yang, Qiao Yang, Qiao Yang, Juan Huang, Juan Huang, Juan Huang, Juan Huang, Xumin Ou, Xumin Ou, Xumin Ou, Xumin Ou, Di Sun, Di Sun, Di Sun, Di Sun, Bin Tian, Bin Tian, Bin Tian, Bin Tian, Zhen Wu, Zhen Wu, Zhen Wu, Zhen Wu, Yu He, Yu He, Yu He, Yu He, Anchun Cheng, Anchun Cheng, Anchun Cheng, Anchun Cheng   +65 more
doaj   +1 more source

Promiscuous stimulation of HSP70 ATPase activity by parasite‐derived J‐domains

FEBS Open Bio, EarlyView.
The malaria parasite Plasmodium falciparum exports three highly homologous yet functionally divergent J‐domain proteins into human erythrocytes. Here, we show that J‐domains isolated from all three proteins effectively stimulate the ATPase activity of both endogenous host and exported parasite HSP70 chaperones.
Julian Barth, Moritz Koch, Le‐Han Rössner, Johanna Eichhorn, Denys Pogoryelov, Matthias P. Mayer, Jude M. Przyborski   +6 more
wiley   +1 more source

Real-time plasmid transmission detection pipeline

Microbiology Spectrum
The spread of antimicrobial resistance among bacteria by horizontal plasmid transmissions poses a major challenge for clinical microbiology. Here, we evaluate a new real-time plasmid transmission detection pipeline implemented in the SeqSphere+ (Ridom ...
Natalie Scherff, Jörg Rothgänger, Thomas Weniger, Alexander Mellmann, Dag Harmsen   +4 more
doaj   +1 more source

Construction of a novel fungal gus expression plasmid, and its evaluation in Aspergillus nidulans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Genetics at Massey University [PDF]

, 1996
A GUS expression plasmid, pFunGus, was constructed containing a multi-cloning site for the insertion of gene regulatory elements, to be used in fungal reporter gene studies.
McGowan, Tania Louise
core  

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dissemination of IncQ1 Plasmids Harboring NTE_KPC-IId in a Brazilian Hospital

Microorganisms
KPC is a clinically significant serine carbapenemase in most countries, and its rapid spread threatens global public health. blaKPC transmission is commonly mediated by Tn4401 transposons.
Camila Maria dos Santos Boralli, Julian Andres Paganini, Rodrigo Silva Meneses, Camila Pacheco Silveira Martins da Mata, Edna Marilea Meireles Leite, Anita C. Schürch, Fernanda L. Paganelli, Rob J. L. Willems, Ilana L. B. C. Camargo   +8 more
doaj   +1 more source

Exon 7 splicing of ERα predicts poor prognosis and increases phenotypic heterogeneity in luminal a subtype breast cancer

FEBS Open Bio, EarlyView.
ERα splice variant ERα∆7 lacks the C‐terminus, and its expression may change phenotypes of breast cancers. Our results showed that ERα∆7 is found in the luminal A subtype, and elevated ERα∆7 levels are linked to improved cell survival with lower proliferation and migration.
Long Wai Tsui, Taobo Hu, Thomson Christian Husein, Wai Hei Shek, Bingrong Chen, Depeng Wang, Shu Wang, Weichuan Yu, Xiaodan Fan, Mengping Long, Toyotaka Ishibashi   +10 more
wiley   +1 more source