Results 21 to 30 of about 1,044,031 (345)

Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli

Frontiers in Microbiology, 2022
Horizontal gene transfer plays an important role in the spread of antibiotic resistance, in which plasmid-mediated conjugation transfer is the most important mechanism.
Manlin Ding, Manlin Ding, Zi Ye, Lu Liu, Wei Wang, Qiao Chen, Feiyang Zhang, Ying Wang, Åsa Sjöling, Alberto J. Martín-Rodríguez, Renjing Hu, Wenbi Chen, Yingshun Zhou   +12 more
doaj   +1 more source

Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates

mSystems, 2019
Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples ...
Ankita Kothari, Drishti Soneja, Albert Tang, Hans K. Carlson, Adam M. Deutschbauer, Aindrila Mukhopadhyay   +5 more
doaj   +1 more source

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Frontiers in Microbiology, 2020
The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance
Yan Li, Qian Wang, Kai Peng, Yuan Liu, Yuan Liu, Yuan Liu, Ruichao Li, Ruichao Li, Ruichao Li, Zhiqiang Wang, Zhiqiang Wang   +10 more
doaj   +1 more source

High-efficiency transformation of mammalian cells by plasmid DNA.

Molecular and Cellular Biology, 1987
We describe a simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells. In this protocol, the calcium phosphate-DNA complex is formed gradually in the medium during incubation ...
Claudia A. Chen, H. Okayama
semanticscholar   +1 more source

Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to Invasive Chlamydia trachomatis Biovars

mBio, 2018
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease. C. trachomatis isolates are classified into 2 biovars—lymphogranuloma venereum (LGV) and trachoma—which are ...
Michael John Patton, Chih-Yu Chen, Chunfu Yang, Stuart McCorrister, Chris Grant, Garrett Westmacott, Xin-Yong Yuan, Estela Ochoa, Robert Fariss, William M. Whitmire, John H. Carlson, Harlan D. Caldwell, Grant McClarty   +12 more
doaj   +1 more source

Subinhibitory Concentration of Colistin Promotes the Conjugation Frequencies of Mcr-1- and blaNDM-5-Positive Plasmids

Microbiology Spectrum, 2022
Horizontal gene transfer (HGT) plays a significant role in the spread of antibiotic resistance genes (ARGs). Most reported compounds promote HGT by increasing the cell membrane permeability.
Xia Xiao, Fuxin Zeng, Ruichao Li, Yuan Liu, Zhiqiang Wang   +4 more
doaj   +1 more source

Comparative analysis of Listeria monocytogenes plasmid transcriptomes reveals common and plasmid‐specific gene expression patterns and high expression of noncoding RNAs

MicrobiologyOpen, 2022
Recent research demonstrated that some Listeria monocytogenes plasmids contribute to stress survival. However, only a few studies have analyzed gene expression patterns of L. monocytogenes plasmids.
Justin M. Anast, Andrea J. Etter, Stephan Schmitz‐Esser   +2 more
doaj   +1 more source

Iteron Plasmids

Microbiology Spectrum, 2014
ABSTRACT Iteron-containing plasmids are model systems for studying the metabolism of extrachromosomal genetic elements in bacterial cells. Here we describe the current knowledge and understanding of the structure of iteron-containing replicons, the structure of the iteron plasmid encoded replication initiation proteins, and the molecular ...
Igor, Konieczny, Katarzyna, Bury, Aleksandra, Wawrzycka, Katarzyna, Wegrzyn   +3 more
openaire   +2 more sources

Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans.

Proceedings of the National Academy of Sciences of the United States of America, 1979
pRK212.2, a derivative of the broad host range plasmid RK2, contains two EcoRI cleavage fragments, A and B, neither of which can replicate by itself in Escherichia coli.
D. Figurski, D. Helinski
semanticscholar   +1 more source

In silico characterization of blaNDM-harboring plasmids in Klebsiella pneumoniae

Frontiers in Microbiology, 2022
Klebsiella pneumoniae is a primary culprit of antibiotic-resistant nosocomial infections worldwide, and infections caused by NDM-producing strains are a major threat due to limited therapeutic options.
Zhu Zeng, Lei Lei, Linman Li, Shengni Hua, Wenting Li, Limei Zhang, Qiuping Lin, Zhixiong Zheng, Jing Yang, Xiaohui Dou, Luan Li, Xiaobin Li   +11 more
doaj   +1 more source