Results 21 to 30 of about 1,449,118 (412)

DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations

Molecular Therapy: Nucleic Acids, 2016
Adeno-associated viral (AAV) vectors are considered as one of the most promising delivery systems in human gene therapy. In addition, AAV vectors are frequently applied tools in preclinical and basic research. Despite this success, manufacturing pure AAV
Maria Schnödt, Marco Schmeer, Barbara Kracher, Christa Krüsemann, Laura Escalona Espinosa, Anja Grünert, Thomas Fuchsluger, Anja Rischmüller, Martin Schleef, Hildegard Büning   +9 more
doaj   +1 more source

Molecular Characterization of blaNDM-Carrying IncX3 Plasmids: blaNDM-16b Likely Emerged from a Mutation of blaNDM-5 on IncX3 Plasmid

Microbiology Spectrum, 2022
Dissemination of blaNDM, which is carried on the IncX3 plasmid, among Enterobacterales has been reported worldwide. In particular, blaNDM-5-carrying IncX3 plasmids can spread among several hosts, facilitating their dissemination.
Tsukasa Ariyoshi, Kotaro Aoki, Hiroaki Kubota, Kenji Sadamasu, Yoshikazu Ishii, Kazuhiro Tateda   +5 more
doaj   +1 more source

A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

Nucleic Acids Research, 1979
A procedure for extracting plasmid DNA from bacterial cells is described. The method is simple enough to permit the analysis by gel electrophoresis of 100 or more clones per day yet yields plasmid DNA which is pure enough to be digestible by restriction ...
H. Birnboim, J. Doly
semanticscholar   +1 more source

Remarkable stability of an instability-prone lentiviral vector plasmid in Escherichia coli Stbl3 [PDF]

, 2012
Large-scale production of plasmid DNA to prepare therapeutic gene vectors or DNA-based vaccines requires a suitable bacterial host, which can stably maintain the plasmid DNA during industrial cultivation.
Al-Allaf, FA, Mehmet, H, Tchetchelnitski, V, Tolmachov, OE, Zambetti, LP   +4 more
core   +1 more source

Subinhibitory antibiotic concentrations promote the horizontal transfer of plasmid-borne resistance genes from Klebsiellae pneumoniae to Escherichia coli

Frontiers in Microbiology, 2022
Horizontal gene transfer plays an important role in the spread of antibiotic resistance, in which plasmid-mediated conjugation transfer is the most important mechanism.
Manlin Ding, Manlin Ding, Zi Ye, Lu Liu, Wei Wang, Qiao Chen, Feiyang Zhang, Ying Wang, Åsa Sjöling, Alberto J. Martín-Rodríguez, Renjing Hu, Wenbi Chen, Yingshun Zhou   +12 more
doaj   +1 more source

Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin

Frontiers in Microbiology, 2020
The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance
Yan Li, Qian Wang, Kai Peng, Yuan Liu, Yuan Liu, Yuan Liu, Ruichao Li, Ruichao Li, Ruichao Li, Zhiqiang Wang, Zhiqiang Wang   +10 more
doaj   +1 more source

High-efficiency transformation of mammalian cells by plasmid DNA.

Molecular and Cellular Biology, 1987
We describe a simple calcium phosphate transfection protocol and neo marker vectors that achieve highly efficient transformation of mammalian cells. In this protocol, the calcium phosphate-DNA complex is formed gradually in the medium during incubation ...
Claudia A. Chen, H. Okayama
semanticscholar   +1 more source

Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to Invasive Chlamydia trachomatis Biovars

mBio, 2018
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease. C. trachomatis isolates are classified into 2 biovars—lymphogranuloma venereum (LGV) and trachoma—which are ...
Michael John Patton, Chih-Yu Chen, Chunfu Yang, Stuart McCorrister, Chris Grant, Garrett Westmacott, Xin-Yong Yuan, Estela Ochoa, Robert Fariss, William M. Whitmire, John H. Carlson, Harlan D. Caldwell, Grant McClarty   +12 more
doaj   +1 more source

Subinhibitory Concentration of Colistin Promotes the Conjugation Frequencies of Mcr-1- and blaNDM-5-Positive Plasmids

Microbiology Spectrum, 2022
Horizontal gene transfer (HGT) plays a significant role in the spread of antibiotic resistance genes (ARGs). Most reported compounds promote HGT by increasing the cell membrane permeability.
Xia Xiao, Fuxin Zeng, Ruichao Li, Yuan Liu, Zhiqiang Wang   +4 more
doaj   +1 more source

Plasmids of corynebacteria [PDF]

FEMS Microbiology Letters, 1999
Corynebacteria are pleomorphic, asporogenous, Gram-positive bacteria. Included in this group are nonpathogenic soil corynebacteria, which are widely used for the industrial production of amino acids and detergents, and in biotransformation of steroids. Other members of this group are plant and animal pathogens.
Niharika Nath, J. K. Deb
openaire   +3 more sources