Results 61 to 70 of about 1,390,499 (412)
Eigensolutions and spectral analysis of a model for vertical gene transfer of plasmids [PDF]
, 2018 Plasmids are autonomously replicating genetic elements in bacteria. At cell division plasmids are distributed among the two daughter cells. This gene transfer from one generation to the next is called vertical gene transfer. We study the dynamics of a bacterial population carrying plasmids and are in particular interested in the long-time distribution ...
arxiv +1 more source
Emergence of Carbapenem- and Tigecycline-Resistant Proteus cibarius of Animal Origin
Frontiers in Microbiology, 2020 The emergence of tet(X) and carbapenemase genes in Enterobacterales pose significant challenges to the treatment of infectious diseases. Convergence of these two categories of genes in an individual pathogen would deteriorate the antimicrobial resistance
Yan Li +10 more
doaj +1 more source
Emergence of plasmid stability under non-selective conditions maintains antibiotic resistance
Nature Communications, 2019 Plasmid acquisition is an important mechanism of rapid adaptation and niche expansion in prokaryotes. Positive selection for plasmid-coded functions is a major driver of plasmid evolution, while plasmids that do not confer a selective advantage are ...
Tanita Wein +3 more
semanticscholar +1 more source
Frontiers in Microbiology, 2022 Klebsiella pneumoniae exhibits extensive phenotypic and genetic diversity. Higher plasmid loads in the cell were supposed to play an key role in its genome diversity.
Mufeng Cai +23 more
doaj +1 more source
Asymmetrical inheritance of plasmids depends on dynamic cellular geometry and volume exclusion effects [PDF]
, 2015 The asymmetrical inheritance of plasmid DNA, as well as other cellular components, has been shown to be involved in replicative aging. In Saccharomyces cerevisiae, there is an ongoing debate regarding the mechanisms underlying this important asymmetry. Currently proposed models suggest it is established via diffusion, but differ on whether a diffusion ...
arxiv +1 more source
Plasmid detection and assembly in genomic and metagenomic data sets
Genome Research, 2019 Although plasmids are important for bacterial survival and adaptation, plasmid detection and assembly from genomic, let alone metagenomic, samples remain challenging.
D. Antipov +3 more
semanticscholar +1 more source
Frontiers in Microbiology, 2022 Microorganisms have developed many strategies in the process of long-term defense against external attacks, one of which is the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) bacterial immunological ...
Yuqiao Zhou +10 more
doaj +1 more source
Generating site saturation mutagenesis libraries and transferring them to broad host range plasmids using type IIS restriction enzymes [PDF]
arXiv, 2023 Protein engineering is an established method for tailoring enzymatic reactivity. A commonly used method is directed evolution, where the mutagenesis and natural selection process is mimicked and accelerated in the laboratory. Here, we describe a reliable method for generating saturation mutagenesis libraries by golden gate cloning in a broad host range
arxiv
Persistence and reversal of plasmid-mediated antibiotic resistance
Nature Communications, 2017 In the absence of antibiotic-mediated selection, sensitive bacteria are expected to displace their resistant counterparts if resistance genes are costly. However, many resistance genes persist for long periods in the absence of antibiotics.
A. Lopatkin +5 more
semanticscholar +1 more source
FEBS Letters, EarlyView.The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley +2 more
wiley +1 more source