Retraction Notice: "MUC1 expressing tumor growth was retarded after human mucin 1 (MUC1) plasmid DNA immunization". [PDF]
Int J Immunopathol Pharmacoleuropepmc +1 more source
Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation. [PDF]
Bio ProtocSiamalube B +4 more
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Intravitreal Delivery of PEGylated-ECO Plasmid DNA Nanoparticles for Gene Therapy of Stargardt Disease. [PDF]
Pharm ResSun D +6 more
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Pulsed Electric Fields Induce STING Palmitoylation and Polymerization Independently of Plasmid DNA Electrotransfer. [PDF]
PharmaceuticsSales Conniff A +3 more
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RecA-dependent or independent recombination of plasmid DNA generates a conflict with the host EcoKI immunity by launching restriction alleviation. [PDF]
Nucleic Acids ResSkutel M +6 more
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Methods to improve the immunogenicity of plasmid DNA vaccines
Drug Discovery Today, 2021DNA vaccines have emerged as innovative approaches that have great potential to overcome the limitations of current conventional vaccines. Plasmid DNA vaccines are often safer than other vaccines {AuQ: Edit OK?} because they carry only antigen genetic ...
Angela Sousa +2 more
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Genes that code for the production of protein antigens have been cloned and recombined with plasmids. Gene-plasmid constructs have been amplified in a bacterial host, purified and administered to a mammalian host. The gene is expressed in the host and the antigen that is produced induces an immune response.
G, Mor, M, Eliza
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Dephosphorylation of Plasmid DNA
Cold Spring Harbor Protocols, 2020This protocol presents a method to remove 5′-phosphate residues from protruding or blunt termini of linearized plasmids. This suppresses the recircularization of plasmid DNA during subsequent ligation procedures.
Michael R, Green, Joseph, Sambrook
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The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vectors.A prerequisite for the success of plasmid-based therapies is the development of cost-effective and ...
Joachim, Stadler +2 more
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Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations.
M M, Diogo +2 more
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