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Pathologic Tissue Injury and Inflammation in Mice Immunized with Plasmid DNA-Encapsulated DOTAP-Based Lipid Nanoparticles. [PDF]

open access: yesBioconjug Chem
Peng S   +11 more
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Dephosphorylation of Plasmid DNA

Cold Spring Harbor Protocols, 2020
This protocol presents a method to remove 5′-phosphate residues from protruding or blunt termini of linearized plasmids. This suppresses the recircularization of plasmid DNA during subsequent ligation procedures.
Michael R, Green, Joseph, Sambrook
openaire   +2 more sources

Plasmid DNA purification

The Journal of Gene Medicine, 2004
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vectors.A prerequisite for the success of plasmid-based therapies is the development of cost-effective and ...
Joachim, Stadler   +2 more
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Chromatography of plasmid DNA

Journal of Chromatography A, 2005
Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations.
M M, Diogo   +2 more
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Quick Transformation with Plasmid DNA

Cold Spring Harbor Protocols, 2022
Genomic engineering of Escherichia coli and Salmonella often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches of competent cells for storage purposes unnecessary.
Nara Figueroa-Bossi   +2 more
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The Ribosomal DNA Plasmids of Entamoeba

Parasitology Today, 1998
In most organisms, the nuclear ribosomal RNA (rRNA) genes are highly repetitive and arranged as tandem repeats on one or more chromosomes. In Entamoeba, however, these genes are located almost exclusively on extrachromosomal circular DNA molecules with no clear evidence so far of a chromosomal copy.
S, Bhattacharya, I, Som, A, Bhattacharya
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In vivo plasmid DNA electrotransfer

Current Opinion in Biotechnology, 2002
In vivo electrotransfer is a physical technique for gene delivery in various mammalian tissues, which involves the injection of plasmid DNA into a target tissue and administration of an electric field. Its ease of performance, as well as recent understanding of its mechanism and applications to different mammalian tissues such as skeletal muscle, liver,
Pascal, Bigey   +2 more
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