Results 241 to 250 of about 2,326,635 (307)

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing

Stratis N, Morbidelli M, Kiparissides A.
europepmc   +1 more source

Plasmid DNA Delivery to Cancer Cells with Poly(L-lysine)-Based Copolymers Bearing Thermally Sensitive Segments: Balancing Polyplex Tightness, Transfection Efficiency, and Biocompatibility. [PDF]

Pharmaceutics
Kotmakci M, Toncheva-Moncheva N, Tarkavannezhad S, Debelec Butuner B, Dimitrov I, Rangelov S.   +5 more
europepmc   +1 more source

Retraction for Ruby and Novick, Plasmid interactions in <i>Staphylococcus aureus</i>: Nonadditivity of compatible plasmid DNA pools. [PDF]

Proc Natl Acad Sci U S A
europepmc   +1 more source

Extracellular vesicle mimetics as delivery vehicles for oligonucleotide-based therapeutics and plasmid DNA. [PDF]

Front Bioeng Biotechnol
Oshchepkova A, Chernikov I, Miroshnichenko S, Patutina O, Markov O, Savin I, Staroseletz Y, Meschaninova M, Puchkov P, Zhukov S, Kupryushkin M, Maslov M, Sen'kova A, Vlassov V, Chernolovskaya E, Zenkova M.   +15 more
europepmc   +1 more source

Rapid quantitative analysis of double-stranded plasmid DNA with capillary gel electrophoresis for applications in quality control and radiation research. [PDF]

Sci Rep
Hahn MB.
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Retraction Notice: "MUC1 expressing tumor growth was retarded after human mucin 1 (MUC1) plasmid DNA immunization". [PDF]

Int J Immunopathol Pharmacol
europepmc   +1 more source

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Dephosphorylation of Plasmid DNA

Cold Spring Harbor Protocols, 2020
This protocol presents a method to remove 5′-phosphate residues from protruding or blunt termini of linearized plasmids. This suppresses the recircularization of plasmid DNA during subsequent ligation procedures.
Michael R, Green, Joseph, Sambrook
openaire   +2 more sources

Plasmid DNA purification

The Journal of Gene Medicine, 2004
The demand for efficient production methods of plasmid DNA (pDNA) has increased vastly in response to rapid advances in the use of pDNA in gene therapy and in vaccines since the advantageous safety concerns associated with non-viral over viral vectors.A prerequisite for the success of plasmid-based therapies is the development of cost-effective and ...
Joachim, Stadler, Raf, Lemmens, Tomas, Nyhammar   +2 more
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Chromatography of plasmid DNA

Journal of Chromatography A, 2005
Liquid chromatography plays a central role in process-scale manufacturing of therapeutic plasmid DNA (pDNA) for gene therapy and DNA vaccination. Apart from its use as a preparative purification step, it is also very useful as an analytical tool to monitor and control pDNA quality during processing and in final formulations.
M M, Diogo, J A, Queiroz, D M F, Prazeres   +2 more
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Quick Transformation with Plasmid DNA

Cold Spring Harbor Protocols, 2022
Genomic engineering of Escherichia coli and Salmonella often requires introducing plasmids into strains obtained during the intermediate stages of the process. Such strains are typically transformed only once, making the preparation of large batches of competent cells for storage purposes unnecessary.
Nara Figueroa-Bossi, Roberto Balbontín, Lionello Bossi   +2 more
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