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Preparation of Bacterial Plasmid DNA

Current Protocols in Neuroscience, 1998
AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
openaire   +3 more sources

Intramuscular injection of plasmid DNA

1995
Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
openaire   +2 more sources

Production of Plasmid DNA as Pharmaceutical

2009
Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information--preferably at the right place, time, and level and, on the other hand, is safe and productive under good manufacturing practices (GMP).
Martin, Schleef, Markus, Blaesen
openaire   +2 more sources

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

Nature, 2010
Josiane E. Garneau   +9 more
semanticscholar   +1 more source

Production of single-stranded plasmid DNA.

Methods in Enzymology, 1987
J. Vieira, J. Messing
semanticscholar   +1 more source

Dephosphorylation of Plasmid DNA

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Plasmid DNA Delivery: Nanotopography Matters.

Journal of the American Chemical Society, 2017
Hao Song   +7 more
semanticscholar   +1 more source

Co-delivery of drugs and plasmid DNA for cancer therapy.

Advanced Drug Delivery Reviews, 2016
P. Teo   +3 more
semanticscholar   +1 more source

Purification of Plasmid DNA by Chromatography

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

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