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Preparation of Bacterial Plasmid DNA
Current Protocols in Neuroscience, 1998AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
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Intramuscular injection of plasmid DNA
1995Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
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Production of Plasmid DNA as Pharmaceutical
2009Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information--preferably at the right place, time, and level and, on the other hand, is safe and productive under good manufacturing practices (GMP).
Martin, Schleef, Markus, Blaesen
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The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA
Nature, 2010Josiane E. Garneau +9 more
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Production of single-stranded plasmid DNA.
Methods in Enzymology, 1987J. Vieira, J. Messing
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Dephosphorylation of Plasmid DNA
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Supercoil Sequencing: A Fast and Simple Method for Sequencing Plasmid DNA
DNA, 1985E. Chen, P. Seeburg
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Plasmid DNA Delivery: Nanotopography Matters.
Journal of the American Chemical Society, 2017Hao Song +7 more
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Co-delivery of drugs and plasmid DNA for cancer therapy.
Advanced Drug Delivery Reviews, 2016P. Teo +3 more
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Purification of Plasmid DNA by Chromatography
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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