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Phytoplasma Plasmid DNA Extraction

2012
Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T, Andersen, Lia W, Liefting
openaire   +2 more sources

Activation of spleen lymphocytes by plasmid DNA

Vaccine, 1999
It was shown that plasmid pUC19 DNA stimulates in vitro proliferation of CBA mouse splenocytes in a dose-dependent manner. Stimulation effect of the plasmid DNA is additive with COn A or LPS, synergistic with PMA and is inhibited by nonimmunogenic phosphodiester oligonucleotides and Fab fragments of antimouse Ig antibodies.
E Y, Rykova   +2 more
openaire   +3 more sources

Production of Plasmid DNA as Pharmaceutical

2009
Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information--preferably at the right place, time, and level and, on the other hand, is safe and productive under good manufacturing practices (GMP).
Martin, Schleef, Markus, Blaesen
openaire   +2 more sources

Intramuscular injection of plasmid DNA

1995
Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
openaire   +2 more sources

Dephosphorylation of Plasmid DNA

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions

Ca-A Cancer Journal for Clinicians, 2021
Heather Parsons   +2 more
exaly  

Purification of Plasmid DNA by Chromatography

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Plasmid DNA Supercoiling by DNA Gyrase

2003
P J, Sayer   +3 more
openaire   +2 more sources

Methods to improve the immunogenicity of plasmid DNA vaccines

Drug Discovery Today, 2021
Ângela Sousa   +2 more
exaly  

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