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Phytoplasma Plasmid DNA Extraction
2012Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T, Andersen, Lia W, Liefting
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Activation of spleen lymphocytes by plasmid DNA
Vaccine, 1999It was shown that plasmid pUC19 DNA stimulates in vitro proliferation of CBA mouse splenocytes in a dose-dependent manner. Stimulation effect of the plasmid DNA is additive with COn A or LPS, synergistic with PMA and is inhibited by nonimmunogenic phosphodiester oligonucleotides and Fab fragments of antimouse Ig antibodies.
E Y, Rykova +2 more
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Production of Plasmid DNA as Pharmaceutical
2009Developments in gene therapy, cell therapy, and DNA vaccination require a pharmaceutical gene vector that, on one hand, fulfils the properties to express the encoded information--preferably at the right place, time, and level and, on the other hand, is safe and productive under good manufacturing practices (GMP).
Martin, Schleef, Markus, Blaesen
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Intramuscular injection of plasmid DNA
1995Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
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Dephosphorylation of Plasmid DNA
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions
Ca-A Cancer Journal for Clinicians, 2021Heather Parsons +2 more
exaly
Purification of Plasmid DNA by Chromatography
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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Methods to improve the immunogenicity of plasmid DNA vaccines
Drug Discovery Today, 2021Ângela Sousa +2 more
exaly
Purification of Supercoiled Plasmid DNA
2003K L, Jordan, D L, Evans, D J, Hall
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