Results 261 to 270 of about 2,233,350 (305)

Preparation of Plasmid DNA

Current Protocols in Protein Science, 1998
AbstractThis appendix presents basic procedures for alkaline lysis minipreps, both in tubes and in microtiter plates, and also provides an for boiling lysis. A describes storage of plasmid DNA.
J, Engebrecht, R, Brent
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Production of Plasmid DNA as Pharmaceutical

2015
Pharmaceutical applications of plasmid DNA require certain quality standards, depending on the intended use of the plasmids. That is, for direct gene transfer into human, GMP Grade is mandatory, however, for GMP production of for example viral vectors (AAV or mRNA etc.), the plasmid DNA used has not to be produced under GMP necessarily.
Marco, Schmeer, Martin, Schleef
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Triplex DNA in plasmids and chromosomes

Gene, 1989
Circular plasmids containing pyrimidine purine tracts can form both inter-and intramolecular triplexes. Addition of poly(dTC) to plasmid pTC45, which contains a (TC)45.(GA)45 insert, results in intermolecular triplex formation. Agarose-gel electrophoresis gives rise to many well-resolved bands, which correspond to 1, 2, 3, 4...
J S, Lee, L J, Latimer, B L, Haug, D E, Pulleyblank, D M, Skinner, G D, Burkholder   +5 more
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Pharmacokinetics of Plasmid DNA in the Rat

Pharmaceutical Research, 2001
The pharmacokinetics of plasmid DNA after IV bolus administration in the rat by following supercoiled (SC), open circular (OC), and linear (L) pDNA forms of the plasmid.SC, OC, and L pDNA were injected at 2,500, 500, 333, and 250 microg doses.
B E, Houk, R, Martin, G, Hochhaus, J A, Hughes   +3 more
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Affinity Adsorption of Plasmid DNA

Biotechnology Progress, 2004
The development of a protein-mediated dual functional affinity adsorption of plasmid DNA is described in this work. The affinity ligand for the plasmid DNA comprises a fusion protein with glutathione-S-transferase (GST) as the fusion partner with a zinc finger protein.
Ghose, Siddhartha, Forde, Gareth M., Slater, Nigel K. H.   +2 more
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Radioprotection of plasmid DNA by oligolysines

International Journal of Radiation Biology, 2004
To define ionic conditions under which oligolysines condense DNA as assayed by radioprotection of a plasmid substrate. And to compare these conditions with those required by the well-characterized ligands spermidine and hexammine cobalt (III). This will enable a reversible compaction model for plasmid DNA to be devised that models more closely ...
G L, Newton, A, Ly, N Q, Tran, J F, Ward, J R, Milligan   +4 more
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Introduction of Plasmid DNA into Cells

Current Protocols in Protein Science, 1998
AbstractThis appendix presents a procedure for transformation using calcium chloride, and also an alternate procedure for one‐step preparation and transformation of competent cells.
C E, Seidman, K, Struhl
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Preparation of Bacterial Plasmid DNA

Current Protocols in Neuroscience, 1998
AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
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Intramuscular injection of plasmid DNA

1995
Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
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Phytoplasma Plasmid DNA Extraction

2012
Phytoplasma plasmids have generally been detected from DNA extracted from plants and insects using methods designed for the purification of total phytoplasma DNA. Methods include extraction from tissues that are high in phytoplasma titre, such as the phloem of plants, with the use of CsCl-bisbenzimide gradients that exploit the low G+C content of ...
Mark T, Andersen, Lia W, Liefting
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