Results 271 to 280 of about 982,599 (302)

Plasmid DNA Delivery: Nanotopography Matters

Journal of the American Chemical Society, 2017
Plasmid DNA molecules with unique loop structures have widespread bioapplications, in many cases relying heavily on delivery vehicles to introduce them into cells and achieve their functions. Herein, we demonstrate that control over delicate nanotopography of silica nanoparticles as plasmid DNA vectors has significant impact on the transfection ...
Hao Song, Meihua Yu, Yao Lu, Zhengying Gu, Yannan Yang, Min Zhang, Jianye Fu, Chengzhong Yu   +7 more
openaire   +3 more sources

Preparation of Bacterial Plasmid DNA

Current Protocols in Neuroscience, 1998
AbstractThe protocols in this unit describe methods for preparing bacterial plasmid DNA free from chromosomal DNA. The first is an alkaline lysis miniprep suitable for screening a moderate number of bacterial colonies by restriction endonuclease cleavage and agarose gel electrophoresis.
J, Engebrecht, J S, Heilig, R, Brent
openaire   +3 more sources

Small-Scale Plasmid DNA Preparation

2003
For the initial characterization of a recombinant plasmid, it is necessary to determine the size of the plasmid or, preferably, the size and characteristics of the insert itself. A method is therefore required for the simultaneous preparation, from a number of isolates, of plasmid DNA in a state sufficiently pure for restriction enzyme digestion.
J W, Dale, P J, Greenaway
openaire   +2 more sources

Plasmid DNA replication.

Federation proceedings, 1976
Recent studies have provided some insight to the overall characteristics of plasmid replication in bacteria. The ColE 1 and R6K plasmids replicate via a covalently-closed circular intermediate. Replication is initiated at a fixed origin and is unidirectional in the case of ColE 1 and bidirectional for R6K. In the case of the plasmid R6K.
openaire   +1 more source

Intramuscular injection of plasmid DNA

1995
Striated muscle has recently been shown to be an excellent target tissue for gene transfer in vivo. Both skeletal and cardiac muscle are post-mitotic highly stable structures; consequently gene transfer into these tissues produces long-lasting gene expression. A number of gene transfer systems work effectively with skeletal muscle, including retroviral
openaire   +2 more sources

Dephosphorylation of Plasmid DNA

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Circulating tumor DNA in advanced solid tumors: Clinical relevance and future directions

Ca-A Cancer Journal for Clinicians, 2021
Michael L Cheng, Glenn J Hanna, Heather A Parsons   +2 more
exaly  

Plasmid DNA Supercoiling by DNA Gyrase

2003
P J, Sayer, M L, Goble, M, Oram, L M, Fisher   +3 more
openaire   +2 more sources

Cancer epigenetics in clinical practice

Ca-A Cancer Journal for Clinicians, 2023
Veronica Davalos, Manel Esteller
exaly  

DNA plasmids

International Journal of Biochemistry, 1988
openaire   +1 more source