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Imaging plasmodesmata

Protoplasma, 2010
At only 50 nm in diameter, plasmodesmata (PD) are below the limit of resolution of conventional light microscopy. Consequently, much of our current interpretation of the substructure of PD is derived from transmission electron microscopy. However, PD can be imaged with alternative techniques, including field emission scanning electron microscopy and ...
Karen, Bell, Karl, Oparka
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Plasmodesmata 2010: plasmodesmata down under

Protoplasma, 2010
More than 60 attendees from more than a dozen countries attended the International Plasmodesmata Meeting (Plasmodesmata 2010) held in Sydney, Australia. The structure of plasmodesmata continued to attract interest, with particular focus on how technological progress is advancing our ability to identify and characterise proteins associated with ...
Jessica, Fitzgibbon, Anne, Vatén
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Plasmodesmata and plant cytoskeleton

Trends in Plant Science, 2001
Plant cell-to-cell communication is achieved by membranous conduits called plasmodesmata, which bridge the cytoplasm of neighboring cells. A growing body of immunolocalization data shows an association of the cytoskeleton machinery with plasmodesmata.
Aaziz, R., Dinant, Sylvie, Epel, B.L.
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Plasmodesmata at a glance

Journal of Cell Science, 2018
ABSTRACT Plasmodesmata are cytoplasmic communication channels that are vital for the physiology and development of all plants. They facilitate the intercellular movement of various cargos – ranging from small molecules, such as sugars, ions and other essential nutrients and chemicals, to large complex molecules, such as proteins and ...
Ross E. Sager, Jung-Youn Lee
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Isolation of Plasmodesmata

2016
Plasmodesmata (PD) are plasma membrane lined pores that cross the plant cell wall and connect adjacent cells. Plasmodesmata are composed of elements of the endoplasmic reticulum, plasma membrane, cytosol, and cell wall and thus, as multicomposite structures that are embedded in the cell wall, they are notoriously difficult to isolate from whole plant ...
Christine, Faulkner   +1 more
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Plasmodesmata form and function

Current Opinion in Cell Biology, 2004
Intercellular transport via plasmodesmata controls cell fate decisions in plants, and is of fundamental importance in viral movement, disease resistance, and the spread of RNAi signals. Although plasmodesmata appear to be unique to plant cells, they may have structural and functional similarities to the newly discovered tunneling nanotubes that connect
Michelle Lynn, Cilia, David, Jackson
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Evaluating molecular movement through plasmodesmata

2020
Plasmodesmata are membrane-lined cytoplasmic passageways that facilitate the movement of nutrients and various types of molecules between cells in the plant. They are highly dynamic channels, opening or closing in response to physiological and developmental stimuli or environmental challenges such as biotic and abiotic stresses.
Xu, Wang, Ross, Sager, Jung-Youn, Lee
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Ultrastructural analysis of leaf trichome plasmodesmata reveals major differences from mesophyll plasmodesmata

Planta, 1997
Functional studies on molecular transport through plasmodesmata in leaf mesophyll and trichome cells revealed significant differences in their basal size-exclusion limits and their response to microinjected tobacco mosaic virus movement protein (E. Waigmann et al., 1994, Proc. Natl. Acad. Sci. USA 91: 1433-1437; E. Waigmann and P.
E, Waigmann   +4 more
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Plasmodesmata - Virus Interaction

1990
The initial entry and replication of a virus in a host plant occurs in only a small number of cells. Expression of viral symptoms in the plant is a result of the spreading of competent viral genetic material (either as particles or as unencapsidated RNA/DNA) through the host plant. In nonhost plants, the virus either fails to replicate or replicates in
W. J. Lucas   +4 more
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Plasmodesmata – membrane tunnels with attitude

Current Opinion in Plant Biology, 2011
Plasmodesmata are doors in the rigid cell wall. In multicellular tissues, they allow the passage of molecules needed to create physiological gradients and, by closure, symplastic boundaries, which are necessary for the fundamental processes of plant growth, development and defence.
Andrew J, Maule   +2 more
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