Results 61 to 70 of about 5,412,420 (313)

MicroRNAs play a role in human embryonic stem cell differentiation into endothelial cells [PDF]

, 2011
PhDThe past recent years have seen a surge of evidence demonstrating the regulation of microRNAs (miRNAs) in a myriad of vascular biology events such as cardiogenesis.
Luo, Zhenling
core   +2 more sources

RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu, Xin Duan, Fatemeh Peymani, Jia Wang, Chengjia Bao, Chaolong Xu, Ying Zou, Zixuan Zhang, Yunxi Zhang, Tongyue Li, Martin Pavlov, Junling Wang, Minhan Song, Tianyu Song, Xiaodi Han, Mingxi Sun, Danmin Shen, Ruoyu Duan, Huafang Jiang, Manting Xu, Holger Prokisch, Fang Fang   +21 more
wiley   +1 more source

Generation of a human embryonic stem cell line (WAe009-A-3B) carrying homozygous TNNT2 gene knockout by CRISPR/Cas9 editing

Stem Cell Research
The TNNT2 gene encodes cardiac troponin T (cTnT), a critical protein in cardiac muscle contraction. Mutations in TNNT2 are associated with various cardiomyopathies, including hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM), which ...
Didaer Talanaite, Hongyue Wang, Man Qi, Feng Lan   +3 more
doaj   +1 more source

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Cardiac Differentiation of Pluripotent Stem Cells [PDF]

Stem Cells International, 2011
The ability of human pluripotent stem cells to differentiate towards the cardiac lineage has attracted significant interest, initially with a strong focus on regenerative medicine. The ultimate goal to repair the heart by cardiomyocyte replacement has, however, proven challenging. Human cardiac differentiation has been difficult to control, but methods
Rajala, Kristiina, Pekkanen-Mattila, Mari, Aalto-Setälä, Katriina   +2 more
openaire   +4 more sources

Guanosine‐Quadruplex Hydrogels as Dynamic, Mechanically Tunable and Ion Conductive 3D Scaffolds for Cell Culture Applications

Advanced Functional Materials, EarlyView.
Versatile guanosine‐quadruplex (GQ) hydrogels are developed as dynamic biomatrices for cell culture. These scaffolds are fabricated under mild aqueous conditions, present improved stability, tunable mechanical properties within physiological ranges; fibrillar, porous microstructure, ion conductivity, and cytocompatibility.
Neha Thakur, Minye Jin, Pavel Milkin, Deepa Negi, Carla Cofiño Fabres, Hubert Gojzewski, Leonid Ionov, Alla Synytska, Andries van der Meer, Robert Passier, Julieta I. Paez   +10 more
wiley   +1 more source

The effect of in vitro culture on the stability, expansion and neuronal differentiation of human pluripotent cell lines [PDF]

, 2010
Pluripotent cells are defined by their ability to both self-renew and to differentiation into any cell type within the human body. As such, pluripotent cell lines are of great interest as starting material for drug screening and cell therapies for ...
Gillett, M L, Gillett, M.L.
core  

NuRD Suppresses Pluripotency Gene Expression to Promote Transcriptional Heterogeneity and Lineage Commitment [PDF]

, 2012
Transcriptional heterogeneity within embryonic stem cell (ESC) populations has been suggested as a mechanism by which a seemingly homogeneous cell population can initiate differentiation into an array of different cell types.
O'Shaughnessy, Aoife, Bertone, Paul, Leaford, Donna, Brennecke, Philip, Kalkan, Tuezer, Costello, Ita, Nakagawa, Kentaro, Latos, Paulina, Hynes-Allen, Antony, Signolet, Jason, Reynolds, Nicola, Strouboulis, John, Behrens, Axel, Hendrich, Brian, Loos, Remco, Humphreys, Peter, Mansfield, William, Mosaku, Olukunbi   +17 more
core   +2 more sources

History and current status of clinical studies using human pluripotent stem cells

, 2023
15921598The Human Pluripotent Stem Cell Registry established a database of clinical studies using human pluripotent stem cells (PSCs) as starting material for cell therapies.
Glyn Stacey, Sabine C. Mueller, Nancy Mah, Kobold, Sabine, Mah, Nancy Lynne, Nils Bultjer, Bultjer, Nils, Stacey, Glyn Nigel, Mueller, Sabine C., Kurtz, Andreas, Sabine Kobold, Andreas Kurtz   +11 more
core   +1 more source

CRISPR activation enables high-fidelity reprogramming into human pluripotent stem cells [PDF]

, 2022
Conventional reprogramming methods rely on the ectopic expression of transcription factors to reprogram somatic cells into induced pluripotent stem cells (iPSCs).
Yoshihara, Masahito, Sokka, Joonas, Otonkoski, Timo, Balboa, Diego, Weltner, Jere, Warren, Andrew, Trokovic, Ras, Katayama, Shintaro, Sokka, Juho Joonas, Kilpinen, Helena, Kvist, Jouni, Kyttälä, Aija, Stadelmann, Christian, Laiho, Laura, Jouhilahti, Eeva-Mari, Kere, Juha, Sokka, J, Stadelmann, C, Laiho, L, Balboa, D, Katayama, S,, Kvist, J, Kilpinen, H, Jouhilahti, E-M, Yoshihara, M,, Warren, A, Helena Kilpinen, Aija Kyttälä, Christian Stadelmann, Juha Kere, Ras Trokovic, Shintaro Katayama, Andrew Warren, Masahito Yoshihara, Eeva-Mari Jouhilahti, Timo Otonkoski, Diego Balboa, Laura Laiho, Jouni Kvist, Joonas Sokka, Jere Weltner   +40 more
core   +1 more source