Results 31 to 40 of about 437 (143)

Detection of RNA viruses in potato seed-tubers from northern Antioquia (Colombia)

open access: yesRevista Ceres, 2023
The most important viruses infecting potato in Colombia are PVY, PLRV, PVS, PYVV, PVV, PVX, PMTV, and PVB. Unfortunately, recent investigations on the health status of uncertified and certified seed-tubers of Solanum tuberosum cv.
Andrea García   +5 more
doaj   +1 more source

Assessment of the PMTV RPA oligo combination TGB1-P1F1R3 to sequences in the NCBI GenBank database to determine in silico feasibility of detection.

open access: yes, 2022
The length of each oligo is present below the identifier with the corresponding number of matching nucleotides described for each PMTV isolate under each oligonucleotide. (DOCX)
Keith Schuetz (13184926)   +3 more
core   +1 more source

Detection limits of the RPA assay for potato mop-top virus (PMTV).

open access: yes, 2022
(A) Serial dilution of PMTV RNA in water exhibited uniform amplification and detection from 1 ng/μL to 1 fg/μL. (B) Logarithmic comparison of the onset time to the concentration of RNA template generated a linear prediction curve, allowing for a ...
Keith Schuetz (13184926)   +3 more
core   +1 more source

Transformation of Nicotiana benthamiana with the Potato Mop-Top Virus Coat Protein Gene Produces a Novel Resistance Phenotype Mediated by the Coat Protein

open access: yesMolecular Plant-Microbe Interactions, 1998
Transformed lines of Nicotiana benthamiana expressing a translatable transgene (CPtrans) encoding the coat protein (CP) gene of potato mop-top virus (PMTV) differed considerably in steady-state levels of transgene RNA transcript and CP that were ...
Hugh Barker   +3 more
doaj   +1 more source

Importin-α-mediated nucleolar localization of potato mop-top virus TRIPLE GENE BLOCK1 (TGB1) protein facilitates virus systemic movement, whereas TGB1 self-interaction is required for cell-to-cell movement in Nicotiana benthamiana

open access: yes, 2015
This work was supported by the Swedish Research Council Formas (to E.I.S.), the Carl Tryggers Foundation (to E.I.S. and N.I.L.), the Swedish Institute (to N.I.L.), and the Rural and Environmental Science and Analytical Services Division strategic ...
Savenkov, Eugene I.   +5 more
core   +1 more source

Comparative Transcriptome Profiling of Nicotiana benthamiana Plants Infected with Potato Mop-Top Virus and Its Mutant Lacking a Gene for the 8K Protein Underlines the Role of Chloroplasts During Infection

open access: yesMolecular Plant-Microbe Interactions
Potato mop-top virus (PMTV) is a significant pathogen causing potato “spraing” disease worldwide. The PMTV 8K protein functions as a weak viral suppressor of RNA silencing (VSR), has viroporin activity, and plays a role in pathogenicity by promoting ...
Shweta Roy   +5 more
doaj   +1 more source

RPA XRT screening of oligos for potato mop-top virus (PMTV) detection using RNA from a tuber.

open access: yes, 2022
Sample onset time and intensity are provided as averages for two replicates of each combination. Sample onset time was determined by evaluating change in fluorescence intensity over time, set arbitrarily at 60 RFU/min.
Keith Schuetz (13184926)   +3 more
core   +1 more source

Replication and Movement of In Vitro-Generated Infectious RNAs of Potato Mop-Top Virus RNAs in Two Mechanically Inoculated Potato Cultivars

open access: yesPhytoFrontiers
Potato mop-top virus (PMTV), vectored by soilborne protist Spongospora subterranea f. sp. subterranea is increasingly becoming a threat to potato production in the United States.
Muhammad Azmat Ullah Khan   +3 more
doaj   +1 more source

Potato mop-top virus RNA Can Move Long Distance in the Absence of Coat Protein: Evidence from Resistant, Transgenic Plants

open access: yesMolecular Plant-Microbe Interactions, 2000
Transgenic expression of a translatable version of the Potato mop-top virus (PMTV) coat protein (CP) gene (encoded by RNA 3) in Nicotiana benthamiana prevented production of symptoms and infective virus particles.
Kara D. McGeachy, Hugh Barker
doaj   +1 more source

Trace Element Status (Iron, Zinc, Copper, Chromium, Cobalt, and Nickel) in Iron‐Deficiency Anaemia of Children under 3 Years

open access: yesAnemia, Volume 2014, Issue 1, 2014., 2014
Aim. To determine trace element status and aetiologic factors for development of trace elements deficiencies in children with iron‐deficiency anaemia (IDA) aged 0 to 3 years. Contingent and Methods. 30 patients of the University Hospital, Pleven, Bulgaria—I group; 48 patients of the Sumy Regional Child’s Clinical Hospital, Sumy, Ukraine—II group; 25 ...
Maria Georgieva Angelova   +6 more
wiley   +1 more source

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