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A Prunus necrotic ringspot virus (PNRSV)-Based Viral Vector for Characterization of Gene Functions in Prunus Fruit Trees

2020
Virus-induced gene silencing (VIGS) is a gene silencing mechanism by which an invading virus targets and silences the endogenous genes that have significant sequence similarity with the virus. It opens the door for us to develop viruses as powerful viral vectors and modify them for molecular characterization of gene functions in plants. In the past two
Hongguang, Cui, Yinzi, Li, Aiming, Wang
openaire   +2 more sources

Complete genome sequences of five prunus necrotic ringspot virus isolates from Inner Mongolia of China and comparison to other PNRSV isolates around the world

Journal of Plant Pathology, 2019
Prunus necrotic ringspot virus (PNRSV) is an important virus of stone fruit trees (Prunus sp.). In this study, complete genomic sequences of five PNRSV isolates from P. persica (Pea1 and Pea4), P. avium (Che1 and Che2) and P. cerasifera (P.cerasifera1) in Inner Mongolia of China were determined and analyzed together with other previously published ...
Zheng-Nan Li
exaly   +2 more sources

THE INCIDENCE OF PRUNUS NECROTIC RINGSPOT (PNRSV) AND APPLE CHLOROTIC LEAFSPOT (ACLSV) VIRUSES ON STONE FRUITS GROWN IN EAST ANATOLIA REGION OF TURKEY

Acta Horticulturae, 2006
A number of surveys were carried out in the main stone fruit growing areas of Malatya, Elazig, Igdir, Erzincan and Erzurum provinces of East Anatolia (Turkey) for the detection of Prunus necrotic ringspot (PNRSV) and Apple chlorotic leaf spot (ACLSV) viruses. A total number of 1451 samples were tested by ELISA (1319 apricot, 97 cherry, 17 peach, 9 plum
Sipahioglu H.M., Baloglu S.
exaly   +3 more sources

Molecular characterization of ilarvirus Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) in Spanish autochthonous almond cultivars

Acta Horticulturae, 2018
Traditional almond cultivars are an interesting source of different traits for breeders. These old cultivars have been multiplied by grafting for a long time and usually have been accumulating different viruses. In this work a molecular characterization of the ilarviruses Prunus necrotic ring spot virus (PNRSV) and Prune dwarf virus (PDV) was performed
M. Rubio   +4 more
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Ανάπτυξη δοκιμών real-time PCR για την ανίχνευση των παθογόνων PDV, PNRSV, PPV, PLMVd και ESFY:

2014
Ο ιός του νανισμού των πυρηνοκάρπων (Prune dwarf virus, PDV), ο ιός της νεκρωτικής δακτυλιοειδούς κηλίδωσης των πυρηνοκάρπων (Prunus necrotic ringspot virus,PNRSV), ο ιός της ευλογιάς των πυρηνοκάρπων (Plum pox virus, PPV), το ιοειδές του λανθάνοντος μωσαϊκού της ροδακινιάς (Peach latent mosaic viroid, PLMVd) και το φυτόπλασμα του Ευρωπαϊκού ίκτερου ...
openaire   +1 more source

Doğu Akdeniz bölgesinde mevcut prunus necrotic ringspot virüsünün (PNRSV) saptanması ve tanılanması

2020
Prunus Necrotic Ringspot Virus (PNRSV), kültüre alınmış sert çekirdekli türlerin çoğunu değişen derecelerde infekte ederek ciddi hastalıklara neden olmaktadır. Virüsün aşı, polen ve tohumla taşındığı bilinmektedir. Etmen, fidanlıklarda ciddi hastalıklara neden olabilirken ağaçların düşük standartlarda ve yavaş gelişmesine de neden olmaktadır.
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Detection of Prunus necrotic ringspot virus (PNRSV) in almond and effect on pollen germination

Acta Horticulturae, 2018
A. Nikbakht Dehkordi   +4 more
openaire   +1 more source

Reverz transkriptaz polimeraz zincir reaksiyonu tekniği ile prunus necrotic ringspot virus (PNRSV) izolatının duyarlı tanısının yapılması ve duyarlığının ELISA (enzyme linked immunosorbent assay) testi ile karşılaştırılması

2021
İki farklı teşhis yönteminin (DAS-ELISA ve RT-PCR) odunsu konukçu Prunus mahalefe transfer edilen Malatya izolatı Prunus necrotic ringspot virüsünü (PNRSV) teşhisindeki başansının karşılaştırmalı analizi gerçekleştirilmiştir. RT- PCR testi için total RNA ekstraksiyonu, silika temelli metoda göre taze yaprak, kök, kabuk ve yeşil sürgün kabuğu dokuları ...
openaire   +1 more source

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