Results 101 to 110 of about 12,112,488 (299)

Functional comparison of EncB and EncC cargo proteins in iron storage within the Myxococcus xanthus encapsulin

FEBS Letters, EarlyView.
Encapsulins are protein nanocompartments that play an important role in iron storage. In the Myxococcus xanthus encapsulin system, two cargo proteins called EncB and EncC contribute to iron mineralization. Here, we show that EncB and EncC generate iron‐containing minerals with distinct chemical compositions, suggesting that the composition of stored ...
Harry B. McDowell, Thomas Walther, Egbert Hoiczyk   +2 more
wiley   +1 more source

RESEARCH ON ACCELERATED LOAD SPECTRUM FOR FATIGUE TEST OF PHEV SUBFRAME WELDS

Jixie qiangdu
Aiming at the problem of the weld fatigue test of the new energy vehicle subframe, a program load spectrum compilation method based on the failure dominant load was proposed. Firstly, the finite element model of the rear subframe was established, and the
ZHAO Lihui, WEI Xuguo, LIANG Shan, WENG Shuo, FENG Jinzhi, ZHANG Dongdong   +5 more
doaj  

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Mechanical Behaviors of a New Polymer-Based Restorative Material for Immediate Loading: An In Vitro Comparative Study

Applied Sciences
The aim of the present in vitro comparative study is to validate a novel composite polymer, named “ONLY”, developed to overcome the mechanical drawbacks of conventional, metal-reinforced poly(methyl methacrylate) (PMMA) interim restoration.
Milena Pisano, Łukasz Zadrożny, Anna Di Marzio, Ignazio Kurti, Silvio Mario Meloni, Aurea Immacolata Lumbau, Francesco Mollica, Mario Cesare Pozzan, Santo Catapano, Rafał Maksymilian Molak, Gabriele Cervino, Marco Tallarico   +11 more
doaj   +1 more source

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Functionally-informed fine-mapping identifies genetic variants linking increased CHD1L expression and HIV restriction in monocytes

Scientific Reports
Human Immunodeficiency Virus Type 1 (HIV) set-point viral load is a strong predictor of disease progression and transmission risk. A recent genome-wide association study in individuals of African ancestries identified a region on chromosome 1 ...
Riley H. Tough, Paul J. McLaren, The International Collaboration for the Genomics of HIV   +2 more
doaj   +1 more source

Analysis and Comparison of Three Bending Tests on Phosphogypsum-Based Material According to Peridynamic Theory

Buildings
Phosphogypsum-based materials have gained much attention in the field of road infrastructure from the economic and sustainable perspectives. The Three-point bending test, the Four-point bending test and the Semi-circular bending test are three typical ...
Haoyu Ma, Kai Zhang, Sheng Liang, Jiatian Dong, Xiangyang Fan, Xuemei Zhang   +5 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source

Study on the Time-Varying Stiffness Characteristics of Four-Point Contact Ball Bearings

Lubricants
This paper takes a four-point contact ball bearing of a wind turbine as the research object, analyzes the force and deformation relationship under the combined action of axial load and radial load, obtains the load distribution of rolling elements, and ...
Runlin Chen, Jiakai Li, Yimo Wang, Rushen Deng, Weihao Chen, Wenhui Li   +5 more
doaj   +1 more source