Results 71 to 80 of about 4,674,421 (280)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Design Parameters of Vortex Pumps: A Meta-Analysis of Experimental Studies
Energies, 2017 Vortex pumps can impel solid-containing fluids and are therefore widely applied, from wastewater transport to the food industry. Despite constant efforts to improve vortex pumps, however, they have remained relatively inefficient compared to conventional
Angela Gerlach +3 more
doaj +1 more source
FEBS Open Bio, EarlyView.Time‐restricted feeding (TRF) in mice increased liver fatty acid oxidation and decreased fatty acid biosynthesis. These alterations persisted when TRF was discontinued and the host was infected with Mycobacterium tuberculosis. Pre‐exposure to TRF did not alter tissue (lung and spleen) mycobacterial burden but significantly reduced CD3+ T cells in lungs
Ashish Gupta +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
, 2005 Optical vortices generically arise when optical beams are combined. Recently, we reported how several laser beams containing optical vortices could be combined to form optical vortex loops, links and knots embedded in a light beam (Leach et al 2004 ...
Dennis, MR +13 more
core +1 more source
SciPost PhysicsWe characterize the dynamical instability responsible for the breakdown of regular rows and necklaces of quantized vortices that appear at the interface between two superfluids in relative motion.
Matteo Caldara, Andrea Richaud, Massimo Capone, Pietro Massignan
doaj +1 more source
A transformation of the point vortex equations [PDF]
Physics of Fluids, 2002 A transformation of the point vortex equations is introduced and studied. The transformation substitutes two point vortices for each vortex used in the calculation of induced velocity at a point, in particular in the calculation of the velocity of another vortex in the system.
openaire +2 more sources
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Fast numerical evaluation of flow fields with vortex cells [PDF]
, 2009 A vortex cell (in this paper) is an aerodynamically shaped cavity in the surface of a body, for example a wing, designed specially to trap the separated vortex within it, thus preventing large-scale unsteady vortex shedding from the wing.
Chernyshenko, S. +3 more
core +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source