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Production of pokeweed antiviral proteins nontoxic to cells by Mutagenesis of PAP-cDNA
Journal of Plant Biology, 1998Pokeweed antiviral protein (PAP), one of ribosome inactivating proteins (RIPs), has very strong toxicity both to prokaryotic and eukaryotic cells. To produce mutant PAPs nontoxic to cells, the PAP-cDNA was inserted into a yeast-E.coli shuttle vector under the control of galactose promoter, mutagenized using hydroxylamine, and transformed into yeast ...
Yoonkang Hur +2 more
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Expression characteristics of pokeweed antiviral proteins (PAPs): Two distinct types of proteins
Journal of Plant Biology, 1997Pokeweed antiviral proteins (PAPs) become novel therapeutic agents in relation to application in human viral diseases and cancer, as well as potent tools in plant system for defending viral infection. We have studied the expression characteristics of PAPs in pokeweed plants by western blot analysis.
Hur Yoonkang +2 more
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Pokeweed Antiviral Protein as an Universal Virus Neutralizer
2002Abstract : The objective of this project is to develop strategies which will allow the use of recombinant mutant pokeweed antiviral protein (PAP) as a potential countermeasure against biological warfare attempts employing pathogenic viruses. In order to increase the therapeutic index of the naturally occurring PAP, recombinant PAP mutants with superior
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Effect of N-terminal deletions on the activity of pokeweed antiviral protein expressed in E. coli
Biochimie, 1998Pokeweed antiviral protein (PAP) from Phytolacca americana is a highly specific N-glycosidase removing adenine residues (A4324 in 28S rRNA and A2660 in 23S rRNA) from intact ribosomes of both eukaryotes and prokaryotes. Due to the ribosome impairing activity the gene coding for mature PAP has not been expressed so far in bacteria whereas the full ...
J, Xu +4 more
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Crystal structure of pokeweed antiviral protein from seeds ofPhytolacca americana at 0.25 nm
Science in China Series C: Life Sciences, 1998Crystals of pokeweed antiviral protein (PAP) from seeds ofPhytolacca americana with high diffraction ability were grown from high protein concentration (100 mg/mL) solution at high temperature (33 degrees C). The crystal structure was solved by use of molecular replacement method and refied by use of molecular dynamic method at 0.25 nm to anR factor of
Z, Zeng, L, Jin, H, Li, Z, Hu, D, Wang
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Deguanylation of Human Immunodeficiency Virus (HIV-1) RNA by Recombinant Pokeweed Antiviral Protein
Biochemical and Biophysical Research Communications, 1999Modeling studies, combined with the molecular docking of the trinucleotide GGG into the active site of the deadenylating RNA N-glycosidase pokeweed antiviral protein (PAP), indicated that a guanine base can fit into the active site pocket of PAP without disturbing its unique geometry and is sandwiched between residues Tyr(72) and Tyr(123) very much ...
F, Rajamohan +3 more
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