Results 141 to 150 of about 1,905 (165)

A small RNA targets pokeweed antiviral protein transcript

Physiologia Plantarum, 2015
AbstractRibosome‐inactivating proteins (RIPs) are a class of plant defense proteins with N‐glycosidase activity (EC 3.2.2.22). Pokeweed antiviral protein (PAP) is a Type I RIP isolated from the pokeweed plant, Phytolacca americana, thought to confer broad‐spectrum virus resistance in this plant.
Alexander, Klenov, Kira C M, Neller, Lydia A, Burns, Gabriela, Krivdova, Katalin A, Hudak   +4 more
openaire   +2 more sources

Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds

Acta Crystallographica Section D Biological Crystallography, 1998
Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine.
H M, Li, Z H, Zeng, Z, Hu, D C, Wang
openaire   +2 more sources

Cytotoxicity of pokeweed antiviral protein.

Cytobios, 1989
Pokeweed antiviral protein, a plant protein which inactivates eukaryotic ribosomes, was found to be cytotoxic to both HeLa and Vero cells. Cellular protein synthesis was inhibited by exposure of the cells to microM concentrations of the antiviral protein for 24 h periods or longer.
G M, Aron, J D, Irvin
openaire   +1 more source

Effect of pretreatment of semen with pokeweed antiviral protein on pregnancy outcome in the rabbit model

Fertility and Sterility, 2001
To determine whether artificial insemination of semen pretreated with pokeweed antiviral protein, a 29-kD antihuman immunodeficiency virus (HIV) protein purified from the leaves of Phytolacca americana, has any adverse effects on pregnancy outcome in the rabbit model.Prospective, controlled study.Center for Advanced Preclinical Sciences at the Parker ...
Osmond J D'Cruz
exaly   +3 more sources

Mucosal Toxicity Studies of a Gel Formulation of Native Pokeweed Antiviral Protein

Toxicologic Pathology, 2004
Pokeweed antiviral protein (PAP), a 29-kDa plant-derived protein isolated from Phytolacca americana, is a promising nonspermicidal broad-spectrum antiviral microbicide. This study evaluated the mucosal toxicity potential of native PAP in the in vivo rabbit vaginal irritation model as well as the in vitro reconstituted human vaginal epithelial tissue ...
Osmond J, D'Cruz, Barbara, Waurzyniak, Fatih M, Uckun   +2 more
openaire   +2 more sources

The immunomodulatory activity of the plant proteins Momordica Charantia inhibitor and Pokeweed antiviral protein

International Journal of Immunopharmacology, 1983
The immunological activity of Momordica Charantia inhibitor (MCI) and of Pokeweed antiviral protein (PAP-S), 30,000 daltons plant proteins possessing close similarity to Ricin A chain as inhibitor of protein synthesis, was investigated in mice. In vivo, single nontoxic injections of microgram amount of these substances delayed H2-incompatible skin ...
F, Spreafico, C, Malfiore, M L, Moras, L, Marmonti, S, Filippeschi, L, Barbieri, P, Perocco, F, Stirpe   +7 more
openaire   +2 more sources

A 13-week subchronic intravaginal toxicity study of Pokeweed antiviral protein in mice

Phytomedicine, 2004
Pokeweed antiviral protein (PAP), a 29-kDa plant-derived protein isolated from Phytolacca americana, is a broad-spectrum antiviral agent. PAP shows unique clinical potential to become the active ingredient of a non-spermicidal microbicide because of its potent in vivo anti-HIV activity, non-interference with in vivo sperm functions, and lack of ...
O J, D'Cruz, B, Waurzyniakt, F M, Uckun
openaire   +2 more sources

Plant resistance to fungal infection induced by nontoxic pokeweed antiviral protein mutants

Nature Biotechnology, 1997
Pokeweed antiviral protein (PAP), a 29-kD protein isolated from Phytolacca americana inhibits translation by catalytically removing a specific adenine residue from the large rRNA of the 60S subunit of eukaryotic ribosomes. Transgenic plants expressing PAP are resistant to a broad spectrum of plant viruses.
O, Zoubenko, F, Uckun, Y, Hur, I, Chet, N, Tumer   +4 more
openaire   +2 more sources

High-Level Expression and Purification of Biologically Active Recombinant Pokeweed Antiviral Protein

Protein Expression and Purification, 1999
Pokeweed antiviral protein (PAP) from the leaves of the pokeweed plant, Phytolacca americana, is a naturally occurring single-chain ribosome-inactivating protein, which catalytically inactivates both prokaryotic and eukaryotic ribosomes. The therapeutic potential of PAP has gained considerable interest in recent years due to the clinical use of native ...
F, Rajamohan, C R, Engstrom, T J, Denton, L A, Engen, I, Kourinov, F M, Uckun   +5 more
openaire   +2 more sources

Expression of pokeweed (Phytolacca americana) antiviral protein cDNA in Escherichia coli and its antiviral activity

Physiological and Molecular Plant Pathology, 1993
Abstract Two DNA constructs encoding the pokeweed antiviral protein (PAP), one encoding PAP and its signal peptide and the other encoding PAP, were made using polymerase chain reactions (PCR), inserted into the expression vector pKK233-2 and transformed into Escherichia coli JM109 cells.
Zhao-chun Chen, John F. Antoniw, Quan Lin, Raymond F. White   +3 more
openaire   +1 more source