Results 121 to 130 of about 3,449,279 (150)

Effect of pretreatment of semen with pokeweed antiviral protein on pregnancy outcome in the rabbit model

Fertility and Sterility, 2001
To determine whether artificial insemination of semen pretreated with pokeweed antiviral protein, a 29-kD antihuman immunodeficiency virus (HIV) protein purified from the leaves of Phytolacca americana, has any adverse effects on pregnancy outcome in the rabbit model.Prospective, controlled study.Center for Advanced Preclinical Sciences at the Parker ...
Osmond J D'Cruz, O J D'Cruz
exaly   +3 more sources

Pokeweed antiviral protein inactivates pokeweed ribosomes; implications for the antiviral mechanism

The Plant Journal, 1994
Pokeweed antiviral protein (PAP) and other ribosome‐inactivating proteins (RIPs) had previously been thought to be incapable of attacking conspecific ribosomes, thus having no effect on endogenous processes. This assertion conflicts with a model for PAP’s in vivo antiviral mechanism in which PAP (a cell wall protein) selectively enters virus‐infected ...
M S, Bonness, M P, Ready, J D, Irvin, T J, Mabry   +3 more
openaire   +2 more sources

Expression of pokeweed antiviral proteins in creeping bentgrass

Plant Cell Reports, 2003
Fungal diseases of creeping bentgrass, an important amenity grass used extensively on golf courses, are a serious problem in golf course management. Transgenic approaches to improving disease resistance to fungal diseases are being explored in many species, and in some cases ribosome-inactivating proteins have been found to be effective.
W D, Dai, S, Bonos, Z, Guo, W A, Meyer, P R, Day, F C, Belanger   +5 more
openaire   +2 more sources

The 2·5 Å Structure of Pokeweed Antiviral Protein

Journal of Molecular Biology, 1993
The pokeweed antiviral protein (PAP), isolated from the leaves of Phytolacca americana, is one of a family of plant and bacterial ribosome-inhibiting proteins (RIPs) which act as specific N-glycosidases on rRNA. Here we report the three-dimensional structure of PAP determined to 2.5 A resolution by X-ray crystallography.
A F, Monzingo, E J, Collins, S R, Ernst, J D, Irvin, J D, Robertus   +4 more
openaire   +2 more sources

Pokeweed Antiviral Protein and Its Applications

2000
The genus Phytolacca produces a number of proteins that have antiviral properties. These antiviral proteins are ribosome-inactivating proteins (RIPs) which remove a single adenine from a highly conserved, surface-exposed, stem-loop structure in the large rRNA of eukaryotic and prokaryotic ribosomes.
N E, Tumer, K, Hudak, R, Di, C, Coetzer, P, Wang, O, Zoubenko   +5 more
openaire   +2 more sources

Pokeweed antiviral protein: Ribosome inactivation and therapeutic applications

Pharmacology & Therapeutics, 1992
Pokeweed antiviral protein (PAP) is a ribosome-inactivating protein (RIP) that inactivates ribosomes by the removal of a single adenine from ribosomal RNA. The studies summarized in our review concern the nature and application of this novel therapeutic agent. We describe how researchers continue to elucidate the structure and biologic activity of RIPs.
J D, Irvin, F M, Uckun
openaire   +2 more sources

A small RNA targets pokeweed antiviral protein transcript

Physiologia Plantarum, 2015
AbstractRibosome‐inactivating proteins (RIPs) are a class of plant defense proteins with N‐glycosidase activity (EC 3.2.2.22). Pokeweed antiviral protein (PAP) is a Type I RIP isolated from the pokeweed plant, Phytolacca americana, thought to confer broad‐spectrum virus resistance in this plant.
Alexander, Klenov, Kira C M, Neller, Lydia A, Burns, Gabriela, Krivdova, Katalin A, Hudak   +4 more
openaire   +2 more sources

Isolation and analysis of a genomic clone encoding a pokeweed antiviral protein

Plant Molecular Biology, 1992
Partial cDNAs encoding a pokeweed antiviral protein were obtained by polymerase chain reaction from the poly(A)+ RNA of seeds, leaves, and roots using two specific primers based on the amino acid sequence of a pokeweed antiviral protein from the seeds (PAP-S).
J, Kataoka, N, Habuka, C, Masuta, M, Miyano, A, Koiwai   +4 more
openaire   +2 more sources

Cytotoxicity of pokeweed antiviral protein.

Cytobios, 1989
Pokeweed antiviral protein, a plant protein which inactivates eukaryotic ribosomes, was found to be cytotoxic to both HeLa and Vero cells. Cellular protein synthesis was inhibited by exposure of the cells to microM concentrations of the antiviral protein for 24 h periods or longer.
G M, Aron, J D, Irvin
openaire   +1 more source

Crystallization and preliminary crystallographic analyses of pokeweed antiviral protein from seeds

Acta Crystallographica Section D Biological Crystallography, 1998
Pokeweed antiviral protein from seeds (PAP-S) is a ribosome inactivating protein which has lowest toxicity and highest inhibition activity as opposed to other pokeweed antiviral proteins and its three potential glycosylation sites (10, 44, 255) were shown to bind to N-acetylglucosamine.
H M, Li, Z H, Zeng, Z, Hu, D C, Wang
openaire   +2 more sources