Results 131 to 140 of about 6,321,382 (341)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

Growth and divergence of the polish subregions over 1995–2006: a search for determinants and spatial patterns [PDF]

open access: yes
This paper investigates the presence of sigma and beta convergence between the Polish subregions over 1995–2006. We verify for the absolute convergence, as well as for the convergence conditioned on the stock of physical capital, human capital and the ...
Herbst, Mikolaj, Wójcik, Piotr
core   +1 more source

Universities of the Third Age in Poland. Emerging Model for 21st Century [PDF]

open access: yes, 2013
Main objective of this paper is to describe emergence of a Polish Universities of the Third Age model. These are a multidisciplinary non-formal education centers, which allow formation of positive responses to the challenges of an ageing population ...
Klimczuk, Andrzej
core   +4 more sources

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

open access: yesFEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn   +4 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

Czynniki związane z występowaniem depresji u osób dorosłych. Przegląd systematyczny literatury polskiej w latach 2009-2014 = Factors related to occurrence of depressive disorders in adults. A systematic review of Polish literature in years 2009–2014

open access: yesJournal of Education, Health and Sport, 2016
Jasik Karolina, Jaślikowska Urszula, Zbrojkiewicz Marta, Ślusarska Barbara, Jasińska Magdalena, Grzegorczyk Magdalena, Nowicki Grzegorz Józef. Czynniki związane z występowaniem depresji u osób dorosłych.
Karolina Jasik   +6 more
doaj  

Chameleon sequences reveal structural effects in proteins representing micelle‐like distribution of hydrophobicity

open access: yesFEBS Open Bio, EarlyView.
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman   +4 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Culture and Society [PDF]

open access: yes, 2015
The present book “Poland – History, Culture and Society. Selected Readings” is the third edition of a collection of academic texts written with the intention to accompany the module by providing incoming students with teaching materials that will assist ...
Matuchniak-Krasuska, Anna
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

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