RNA‐Guided Engineering of the Chloroplast Genome Enabled by Plastid‐Expressed Guide RNAs
ABSTRACT Our goal is to develop RNA‐guided engineering of the chloroplast genome using the CRISPR/Cas9 system. We designed chloroplast minigenes to obtain properly sized single guide RNAs (sgRNAs) in tobacco chloroplasts. The sgRNA 5′ end is defined by transcription from an rRNA operon promoter, and its 3′ end by processing a downstream tRNA (trnG) or ...
Malihe Mirzaee +3 more
wiley +1 more source
Optimized Protocol for In Vitro Pollen Germination in Yam (Dioscorea spp.). [PDF]
Mondo JM +4 more
europepmc +1 more source
ABSTRACT Sucrose transport and starch accumulation are crucial carbohydrate metabolic processes in rice, playing essential roles during late pollen development to ensure pollen maturation, fertility and high grain production. However, the molecular mechanisms regulating sucrose transport and starch accumulation during rice pollen development remain ...
Qiuping Li +10 more
wiley +1 more source
The Balance between Different ROS on Tobacco Stigma during Flowering and Its Role in Pollen Germination. [PDF]
Breygina M +3 more
europepmc +1 more source
ABSTRACT Fruit cuticles regulate the diffusion of water, gases and solutes, functioning as essential protective interfaces against environmental and biotic stresses. Engineering cuticle structure and mechanics therefore represents a promising strategy for improving fruit quality and resilience.
Gulab Chand Arya +5 more
wiley +1 more source
Effects of drought and heat stresses during reproductive stage on pollen germination, yield, and leaf reflectance properties in maize (Zea mays L.). [PDF]
Bheemanahalli R +5 more
europepmc +1 more source
ABSTRACT Global food demand is predicted to rise anywhere from 59% to 98% by 2050 because of increasing population. However, the continued depletion of natural resources and increasing biotic and abiotic stresses will continue to pose significant threats to global food security in coming years.
Memoona Khalid +5 more
wiley +1 more source
In Vitro Oxidative Stress Threatening Maize Pollen Germination and Cytosolic Ca2+ Can Be Mitigated by Extracts of Emmer Wheatgrass Biofortified with Selenium. [PDF]
Del Pino AM +5 more
europepmc +1 more source
ABSTRACT Triticale (x Triticosecale Wittmack) is an interspecific hybrid of wheat and rye. It is a promising grain and forage crop that thrives in poorer soils and low‐input farming systems. It can serve as a viable alternative to traditional small grains such as wheat and barley, bringing diversity to crop rotations and enhancing biodiversity in ...
Riccardo Zustovi +17 more
wiley +1 more source
MAP3Kε1/2 Interact with MOB1A/1B and Play Important Roles in Control of Pollen Germination through Crosstalk with JA Signaling in Arabidopsis. [PDF]
Mei J +6 more
europepmc +1 more source

