Results 231 to 240 of about 86,323 (286)

Poly (ADP-ribose) polymerase 1

2022
Poly (ADP-ribose) polymerase 1 is a key player in DNA repair and transcription regulation. The role of PARP1 in DNA damage recognition and protein signaling in response make it integral to genome stability. Beyond this, it regulates gene expression through chromatin remodeling and transcription factor binding.
Fletcher E Bain, Maria Spies, Marc Wold, Adrian Elcock, Anna Malkova, Miles Pufall, Lori Wallrath   +6 more
openaire   +1 more source

Poly(ADP‐ribose) polymerase: Molecular biological aspects

BioEssays, 1991
AbstractA number of roles have been ascribed to poly(ADP‐ribose) polymerase* including involvement in DNA repair, cell proliferation, differentiation and transformation. Cloning of the gene has allowed the development of molecular biological approaches to elucidate the structure and the function(s) of this highly conserved enzyme.
G, de Murcia, J, Ménissier-de Murcia, V, Schreiber   +2 more
openaire   +2 more sources

Purification of Recombinant Poly(ADP-Ribose) Polymerases

2011
The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude extract are separated on a Heparine Sepharose™ column. The PARP-containing fractions are then affinity purified on a 3-
Amé, Jean-Christophe, Kalisch, Thomas, Dantzer, Françoise, Schreiber, Valérie   +3 more
openaire   +2 more sources

Novel poly(ADP-ribose) polymerase-1 inhibitors

Bioorganic & Medicinal Chemistry Letters, 2007
Synthesis and activity of a series of 3-aroyl-derived analogs of novel pyrrolocarbazole 1 as poly(ADP-ribose) polymerase-1 (PARP-1) inhibitors are disclosed.
Derek, Dunn, Jean, Husten, Mark A, Ator, Sankar, Chatterjee   +3 more
openaire   +3 more sources

Poly(ADP-ribose) polymerase: Structure-function relationship

Biochimie, 1995
Dissection of the human poly(ADP-ribose) polymerase (PARP) molecule in terms of its structure-function relationship has proved to be an essential step towards understanding the biological role of poly(ADP-ribosylation) as a cellular response to DNA damage in eukaryotes.
M. Masson, V. Rolli, F. Dantzer, C. Trucco, V. Schreiber, S. Fribourg, M. Molinete, A. Ruf, E. Alves Miranda, C. Niedergang, D. Hunting, B. Gowans, G.E. Schulz, J.Ménissier de Murcia, G. de Murcia   +14 more
openaire   +2 more sources

Poly(ADP-ribose)polymerase Inhibition - Where Now?

Current Medicinal Chemistry, 2005
The poly(ADP-ribose)polymerases (PARPs) catalyse the transfer of ADP-ribose units from the substrate NAD+ to acceptor proteins, biosynthesising polyanionic poly(ADP-ribose) polymers. A major isoform, PARP-1, has been the target for design of inhibitors for over twenty-five years.
Esther C Y, Woon, Michael D, Threadgill
openaire   +2 more sources

Regulatory mechanisms of poly(ADP-ribose) polymerase

Molecular and Cellular Biochemistry, 1999
Here, we describe the latest developments on the mechanistic characterization of poly(ADP-ribose) polymerase (PARP) [EC 2.4.2.30], a DNA-dependent enzyme that catalyzes the synthesis of protein-bound ADP-ribose polymers in eucaryotic chromatin. A detailed kinetic analysis of the automodification reaction of PARP in the presence of nicked dsDNA ...
R, Alvarez-Gonzalez, T A, Watkins, P K, Gill, J L, Reed, H, Mendoza-Alvarez   +4 more
openaire   +2 more sources

Poly(ADP-Ribose) Polymerase Inhibitor Combination Therapy

The Cancer Journal, 2021
Abstract The introduction of poly(ADP-ribose) polymerase (PARP) inhibitors has led to significant improvements in outcome for several cancer types, most notably high-grade serous ovarian cancer. However, in general, benefit is restricted to tumors characterized by either BRCA1/2 mutation or homologous recombination deficiency.
openaire   +2 more sources

Cytological detection of poly (ADP-ribose) polymerase

Experimental Cell Research, 1976
Abstract An attempt was made to demonstrate poly (ADP-ribose) polymerase cytologically. In vitro incorporation from the nucleotide, [ 3 H]NAD was detected in frozen sections of onion embryo and meristematic tissue by autoradiography. In meristematic tissue, there was a correlation between the number of cells displaying intensein vitro incorporation ...
J F, Payne, A K, Bal
openaire   +2 more sources

Poly(ADP-ribose) polymerases: managing genome stability

The International Journal of Biochemistry & Cell Biology, 2005
The importance of poly(ADP-ribose) metabolism in the maintenance of genomic integrity following genotoxic stress has long been firmly established. Poly(ADP-ribose) polymerase-1 (PARP-1) and its catabolic counterpart, poly(ADP-ribose) glycohydrolase (PARG) play major roles in the modulation of cell responses to genotoxic stress.
Mirella L, Meyer-Ficca, Ralph G, Meyer, Elaine L, Jacobson, Myron K, Jacobson   +3 more
openaire   +2 more sources