Results 81 to 90 of about 737,750 (360)

Construction of hyperthermostable d‐allulose 3‐epimerase from Arthrobacter globiformis M30 using the sequence information from Arthrobacter psychrolactophilus

FEBS Open Bio, EarlyView.
d‐Allulose can be produced from d‐fructose by d‐allulose 3‐epimerase. Based on sequence homology information, we successfully engineered thermostable mutants with the protein engineering method. By integrating positive mutations, we constructed an enzyme that exhibits hyperthermostability without a loss in the activity.
Kensaku Shimada, Kouhei Ohtani, Pushpa Kiran Gullapalli, Kazuhiko Ishikawa   +3 more
wiley   +1 more source

β‐Catenin/c‐Myc Axis Modulates Autophagy Response to Different Ammonia Concentrations

Advanced Biology, Volume 9, Issue 3, March 2025.
Ammonia, detoxified by the liver into urea and glutamine, impacts autophagy differently at varying levels. Low ammonia activates autophagy via c‐Myc and β‐catenin, while high levels suppress it. Using Huh7 cells and Spf‐ash mice, c‐Myc's role in cytoprotective autophagy is revealed, offering insights into hyperammonemia and potential therapeutic ...
S. Sergio, B. Spedicato, G. Corallo, A. Inguscio, M. Greco, D. Musarò, D. Vergara, A. F. Muro, G. De Sabbata, L. R. Soria, N. Brunetti Pierri, M. Maffia   +11 more
wiley   +1 more source

Electric-field-enhanced transport in polyacrylamide hydrogel nano-composites [PDF]

arXiv, 2007
Electroosmotic pumping through uncharged hydrogels can be achieved by embedding the polymer network with charged colloidal inclusions. Matos and co-workers (2006) recently used the concept to enhance the diffusion-limited flux of uncharged molecules across polyacrylamide hydrogel membraness for the purpose of improving the performance of biosensors ...
arxiv  

Biologic activity in a fragment of recombinant human interferon α [PDF]

, 1984
To attempt to locate functionally important regions of the interferon (IFN) molecule, recombinant human IFN-α2 was subjected to proteolytic digestion. The bacterial proteinase thermolysin produced two major complementary fragments, HuIFN-α2-(1-110) and ...
Ackerman, Samuel K., Heintzelman, Mark, Hunkapiller, Michael, Zoon, Kathryn, Zur Nedden, Dorothy   +4 more
core  

GEN1 from a thermophilic fungus is functionally closely similar to non-eukaryotic junction-resolving enzymes [PDF]

, 2014
Processing of Holliday junctions is essential in recombination. We have identified the gene for the junction-resolving enzyme GEN1 from the thermophilic fungus Chaetomium thermophilum and expressed the N-terminal 487-amino-acid section.
Agostinho, Alasdair D.J. Freeman, Andersen, Anne-Cécile Déclais, Anton Gartner, Aravind, Bailly, Bennett, Bloom, Boddy, Castor, Cejka, Chaganti, Chen, Clamp, David M.J. Lilley, Duckett, Duckett, Déclais, Ellis, Fekairi, Finger, Fogg, Fogg, Fogg, Fogg, Gaillard, Garner, Giraud-Panis, Giraud-Panis, Greaves, Holliday, Hosfield, Huson, Ip, Komori, Kvaratskhelia, Lee, Lilley, Lorenz, Mak, Matos, Munoz, Orans, Parkinson, Parkinson, Pöhler, Rass, Svendsen, Tsutakawa, Waldo, Wang, White, White, White, White, Wu, Wyatt, Yijin Liu   +58 more
core   +2 more sources

Using a Supramolecular Approach to Engineer Modular Hydrogel Platforms for Culturing Protoplasts – from General Tissue Engineering to Cellular Agriculture

Advanced Biology, EarlyView.
Using supramolecular monomers, various hydrogel culture systems were formulated to culture protoplasts; including 2D, 2.5D, and 3D hydrogels. Depending on the culture platform, bioactive functionalization led to protoplast enlargement (2D and 2.5D) or plasmolysis (3D). This work shows the potential to modularly engineer synthetic platforms for cellular
Maritza M. Rovers, Erik J. Slootweg, Ferdinand C. O. Los, Patricia Y. W. Dankers   +3 more
wiley   +1 more source

Design of novel temperature-resistant and salt-tolerant acrylamide-based copolymers by aqueous dispersion polymerization

Designed Monomers and Polymers, 2022
Development of polymer-based flooding technology to improve oil recovery efficiency, water dispersion copolymerization of acrylamide, cationic monomer methacryloxyethyltrimethyl ammonium chloride (METAC), and anionic monomer acrylic acid (AA) were ...
Xiang Li, Ye Ju, Yongkang Jia, Fenggang Liu, Guangpu Liu, Shuo Wang, Haoyi Wang, Shihua Mao, Jintao Yang, Guangyan Du   +9 more
doaj   +1 more source

Electroblotting onto activated glass. High efficiency preparation of proteins from analytical sodium dodecyl sulfate-polyacrylamide gels for direct sequence analysis [PDF]

, 1986
We have developed a new method for the isolation of proteins for microsequencing. It consists of electrophoretic transfer (electroblotting) of proteins or their cleavage fragments onto activated glass filter paper sheets immediately after separation by ...
Aebersold, Ruedi H., Hood, Leroy E., Kent, Stephen B. H., Teplow, David B.   +3 more
core  

Modulation of apoprotein E secretion in response to receptor-mediated endocytosis in resident and inflammatory macrophages. [PDF]

, 1984
We have determined the effect of various endocytic ligands on the secretion of ApoE by macrophages. ApoE was a major secreted protein of resident macrophages, but BCG-activated macrophages secreted little ApoE and periodate-elicited macrophages secreted ...
Takemura, R, Werb, Z
core   +2 more sources

Key Trends and Insights in Smart Polymeric Skin Wearable Patches

Advanced Engineering Materials, EarlyView.
Intelligent polymers, which respond to various physical and biological stimuli, are explored for the development of skin wearable patches in biomedical applications. Smart polymers, also known as intelligent or stimuli‐responsive polymers, play a crucial role in the development of advanced wearable patches due to their versatility and softness.
Sergio J. Peñas‐Núñez, Rajat Rai, Miryam Criado‐Gonzalez   +2 more
wiley   +1 more source