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Discontinuous polyacrylamide gel electrophoresis of RNA
Analytical Biochemistry, 1972Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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‘Laterally aggregated’ polyacrylamide gels for electrophoresis
ELECTROPHORESIS, 1992AbstractA new method is described for producing highly porous polyacrylamide matrices: polymerization in presence of a preformed hydrophilic polymer. If a standard mixture of monomers (e.g., 5%T, 4%C) is polymerized in presence of, e.g., polyethylene glycol (PEG) 10 kDa, lateral chain aggregation occurs, with formation of large pore sizes.
Righetti P. G. +3 more
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SDS-Polyacrylamide Gel Electrophoresis of Proteins
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide.
Joseph, Sambrook, David W, Russell
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SDS Polyacrylamide Gel Electrophoresis of Proteins
2003Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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Gradient SDS Polyacrylamide Gel Electrophoresis
2003The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate
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Polyacrylamide Temperature Gradient Gel Electrophoresis
2013Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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Preparative electrophoresis on polyacrylamide gel
Analytical Biochemistry, 1964D, RACUSEN, N, CALVANICO
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SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*
Annals of the New York Academy of Sciences, 1964SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
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Neutral Polyacrylamide Gel Electrophoresis
Cold Spring Harbor Protocols, 2006Joseph, Sambrook, David W, Russell
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