Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices. [PDF]
Pan Q, Yamauchi KA, Herr AE.
europepmc +1 more source
NuSAP Safeguards Centriole Integrity to Mediate CEP57‐CEP152 Torus Recruitment for Proper Engagement
This study reveals a novel role for the microtubule stabilizer NuSAP at centrioles. NuSAP depletion destabilizes the centriole's tubulin structure, causing premature disengagement, PCM defects, and mis‐localization of the CEP57‐CEP63‐CEP152 complex. By reinforcing centriole architecture, NuSAP enables early CEP57 loading and initiates a newly proposed ...
Shiyu Zhang +8 more
wiley +1 more source
Validation of blue- and clear-native polyacrylamide gel electrophoresis protocols to characterize mitochondrial oxidative phosphorylation complexes. [PDF]
Aref J +5 more
europepmc +1 more source
Two-Dimensional Polyacrylamide Gel Electrophoresis for Metalloprotein Analysis Based on Differential Chemical Structure Recognition by CBB Dye. [PDF]
Ishikawa J +9 more
europepmc +1 more source
Protein restriction (PR) slows Alzheimer's disease (AD) in mice, and other benefits of PR are due to decreased branched‐chain amino acids (BCAAs). We show that restricting any BCAA has benefits, with sex‐ and BCAA‐specific impacts on pathology, molecular signaling, and cognition.
Reji Babygirija +22 more
wiley +1 more source
A Fluorescence-Based Histidine-Imidazole Polyacrylamide Gel Electrophoresis (HI-PAGE) Method for Rapid and Practical Lipoprotein Profiling and LDL-C Quantification in Clinical Samples. [PDF]
Takenaka Y +3 more
europepmc +1 more source
Detection of Catalase Activity by Polyacrylamide Gel Electrophoresis (PAGE)in Cell Extracts from Pseudomonas aeruginosa. [PDF]
Pezzoni M, Pizarro RA, Costa CS.
europepmc +1 more source
3D Bioprinted Fat‐Myocardium Model Unravels the Role of Adipocyte Hypertrophy in Atrial Dysfunction
A human‐derived 3D bioprinted fat–myocardium model is developed to investigate how adipocyte hypertrophy drives atrial dysfunction in obesity. Palmitate‐induced adipocyte hypertrophy promotes adipose dysfunction that impairs atrial cardiomyocyte metabolism and electrophysiology through both paracrine and direct interactions. This platform recapitulates
Lara Ece Celebi, Pinar Zorlutuna
wiley +1 more source
An Activity-Based Proteomics with Two-Dimensional Polyacrylamide Gel Electrophoresis (2D-PAGE) for Identifying Target Proteases in <i>Arabidopsis</i> Apoplastic Fluid. [PDF]
Matsui S, Matsubayashi Y.
europepmc +1 more source

