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Identification of Structural Proteins of Rhizobium meliloti Temperate Phage 16-3 [PDF]
, 1982 The structural proteins of Rhizobium meliloti temperate phage 16-3 have been analysed by means of polyacrylamide gel electrophoresis, isoelectric focusing and agarose gel electrophoresis.
Duda, Ernő +3 more
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Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling [PDF]
Archives of Biological Sciences, 2011 An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange ...
Asaduzzaman A.K.M. +2 more
doaj +1 more source
Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]
, 2008 QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
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Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]
, 2010 A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
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Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
BioTechniques, 1998 Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj +1 more source
SDS Agarose Gels for Analysis of Proteins
BioTechniques, 1998 A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
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Polyacrylamide gel electrophoresis of soybean seed proteins
Acta Societatis Botanicorum Poloniae, 2015 Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
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Journal of Pharmacopuncture, 2006 Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
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Genomic and proteomic profiling for cancer diagnosis in dogs [PDF]
, 2016 Global gene expression, whereby tumours are classified according to similar gene expression patterns or ‘signatures’ regardless of cell morphology or tissue characteristics, is being increasingly used in both the human and veterinary fields to assist in ...
Morris, Joanna S.
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Silver Staining of 2D Electrophoresis Gels [PDF]
Methods in Molecular Biology -Clifton then Totowa- 893 (2012)
61-73, 2012 Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It -combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. For its use in proteomics, two important additional features must be considered, compatibility with mass spectrometry and ...
arxiv +1 more source