Results 21 to 30 of about 525,236 (336)
Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
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Polyacrylamide gel electrophoresis of soybean seed proteins
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
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Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
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SDS Agarose Gels for Analysis of Proteins
A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
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Power and limitations of electrophoretic separations in proteomics strategies [PDF]
Proteomics can be defined as the large-scale analysis of proteins. Due to the complexity of biological systems, it is required to concatenate various separation techniques prior to mass spectrometry.
Aebersold +225 more
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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay
Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
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Conducting polymer electrodes for gel electrophoresis.
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson +2 more
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Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]
Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
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Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel [PDF]
Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of ...
N. O. Manko +3 more
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