Results 21 to 30 of about 529,768 (342)
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj +1 more source
The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin
Evans, RW, Williams, J
core +2 more sources
Conducting polymer electrodes for gel electrophoresis.
PLoS ONE, 2014 In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson +2 more
doaj +1 more source
Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]
, 1971 Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
core +4 more sources
Harčova Nauka ì Tehnologìâ, 2018 The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo +3 more
doaj +1 more source
Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel [PDF]
The Ukrainian Biochemical Journal, 2022 Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of ...
N. O. Manko +3 more
doaj +1 more source
Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
, 2009 Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi +103 more
core +5 more sources
Preparative electrophoresis of human apolipoprotein E: an improved method.
Journal of Lipid Research, 1990 Apolipoprotein E was isolated from human very low density lipoproteins by a two-step electrophoretic procedure derived from that of Méndez (1982. Anal. Biochem. 126: 403-408).
S Meunier +3 more
doaj +1 more source
Türk Biyokimya Dergisi, 2022 In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat +3 more
doaj +1 more source
Silver Staining of Proteins in 2DE Gels [PDF]
, 2009 Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille +3 more
core +5 more sources