Results 41 to 50 of about 920,072 (304)
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
FEBS Letters, EarlyView.Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source
The serological grouping system for Serpulina (Treponema) hyodysenteriae [PDF]
, 1992 Lipopolysaceharide from serostrains of Serpulina (Treponema) hyodysenteriae for serogroups A to I was characterized using sodium dodecylsulphate polyacrylamide gel electrophoresis and silver staining.
Lau, T.T.A., Hampson, D.J.
core
FEBS Letters, EarlyView.Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav +2 more
wiley +1 more source
Assembly for electrophoresis in polyacrylamide gel
, 2021 For the separation of proteins by electrophoresis in polyacrylamide gel, once the gel is already prepared, the system where to perform the electrophoresis will be assembled, as explained in this video.https://media.upv.es/player/?id=65122530-8273-11eb ...
Yenush, Lynne, Andrés-Colás, Nuria
core
Localization of the genes for tumor necrosis factor and lymphotoxin between the HLA classI and III regions by field inversion gel electrophoresis [PDF]
, 1988 To clarify the position of the TNFA and TNFB genes on the HLA map, we have assigned TNFA to large DNA restriction fragments separated by field inversion gel electrophoresis, which hybridize with either class III- or class I-specific probes as well. These
Bloemer, K +7 more
core +1 more source
Detection of rotavirus genome by new silver staining method
مجله دانشگاه علوم پزشکی گرگان, 2014 Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj
Blue-native PAGE in plants: a tool in analysis of protein-protein interactions
Plant Methods, 2005 Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey +2 more
doaj +1 more source
The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation
FEBS Letters, EarlyView.Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge +6 more
wiley +1 more source
Acta Societatis Botanicorum Poloniae, 2015 SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and ...
Aleksander F. Sikorski
doaj +1 more source
BMC Research Notes, 2021 Objective The advancement of molecular techniques in an era in which high-throughput sequencing has revolutionized biology renders old-fashioned alternatives to high-throughput methods obsolete.
Julissa J. Sánchez-Velásquez +3 more
doaj +1 more source