Results 21 to 30 of about 357,026 (264)
Türk Biyokimya Dergisi, 2022 In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat +3 more
doaj +1 more source
DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS [PDF]
Poljoprivreda, 2011 Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated.
Ivica Strelec +2 more
doaj
An Improved Method for PAGE-based Detection of Phosphorylated Protein in Yeast
Bio-Protocol, 2012 One dimensional polyacrylamide gel electrophoresis has been successfully used to detect protein phosphorylation. This method is very simple and highly reproducible.
Yuehua Wei
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A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1. [PDF]
PLoS ONE, 2017 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research.
Rahul Mahadev Shelake +4 more
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Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.
Journal of Lipid Research, 1988 Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus ...
C Gabelli +3 more
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Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
Apolipophorin III in locusts: purification and characterization.
Journal of Lipid Research, 1988 Three molecular species of apolipophorin III were purified from adult locust hemolymph by gel filtration and ion-exchange chromatography, and named apo-III-a, apo-III-b, and apo-III-c, respectively.
H Chino, M Yazawa
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FEBS Letters, EarlyView.This study reveals that the small GTPase Rab14 is necessary for human papillomavirus (HPV) infection and plays an essential role in the transport of virions to the trans‐Golgi network (TGN). HPV in the early endosome (EE), which harbors GTP‐bound Rab14, is transported to the TGN through the switch of Rab14 from its GTP‐bound to GDP‐bound form.
Yoshiyuki Ishii, Iwao Kukimoto
wiley +1 more source
Modulation of Homer1 EVH1 domain internal dynamics by putative autism‐associated mutations
FEBS Letters, EarlyView.The putative autism‐associated M65I and S97L variants of the EVH1 domain of the postsynaptic scaffold protein Homer1 do not exhibit substantial changes in their overall structure or partner binding. Both of them, but especially the M65I variant, show altered internal dynamics relative to the wild‐type domain on the μs‐ms timescale, indicated by the ...
Fanni Farkas +6 more
wiley +1 more source
The electrophoresis of transferrins in urea/polyacrylamide gels [PDF]
Biochemical Journal, 1980 The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss of iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to ...
Evans, RW, Williams, J
openaire +3 more sources