Results 21 to 30 of about 920,072 (304)

A versatile polyacrylamide gel electrophoresis based sulfotransferase assay

open access: yesBMC Biotechnology, 2010
Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany   +4 more
doaj   +1 more source

A Novel Gaussian Extrapolation Approach for 2D Gel Electrophoresis Saturated Protein Spots [PDF]

open access: yes, 2012
Analysis of images obtained from two-dimensional gel electrophoresis (2D-GE) is a topic of utmost importance in bioinformatics research, since commercial and academic software available currently has proven to be neither completely effective nor fully ...
Caiazzo, A.   +7 more
core   +1 more source

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives

open access: yesBioTechniques, 2008
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
doaj   +1 more source

Analysis of the effects of BrdU on DLKP human lung cancer cells by two-dimensional difference gel electrophoresis and mass spectrometry [PDF]

open access: yes, 2007
Bromodeoxyuridine (BrdU) is a thymidiie analogue that incorporates into DNA of dividing cells during the S-phase of the cell cycle. Previous work in laboratories reported that treatment with lOyM BrdU in the human lung carcinoma cell line (DLKP) resulted
Henry, Michael
core   +2 more sources

Conducting polymer electrodes for gel electrophoresis.

open access: yesPLoS ONE, 2014
In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson   +2 more
doaj   +1 more source

ELECTROPHORETIC SYSTEMS FOR PREPARATIVE FRACTIONATION OF PROTEIN PRECURSORS OF BIOACTIVE PEPTIDES FROM COW’S MILK

open access: yesHarčova Nauka ì Tehnologìâ, 2018
The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo   +3 more
doaj   +1 more source

SDS-polyacrylamide gel electrophoresis of SpGrx3 WT and mutants.

open access: yes, 2023
SDS-polyacrylamide gel electrophoresis of SpGrx3 WT and mutants.
Sei-Heon Jang (2828522)   +3 more
core   +1 more source

Preparative electrophoresis of human apolipoprotein E: an improved method.

open access: yesJournal of Lipid Research, 1990
Apolipoprotein E was isolated from human very low density lipoproteins by a two-step electrophoretic procedure derived from that of Méndez (1982. Anal. Biochem. 126: 403-408).
S Meunier   +3 more
doaj   +1 more source

Comparison of the modified polyacrylamide gradient gel electrophoresis and high-performance liquid chromatography methods in determining LDL size

open access: yesTürk Biyokimya Dergisi, 2022
In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat   +3 more
doaj   +1 more source

DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS [PDF]

open access: yesPoljoprivreda, 2011
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated.
Ivica Strelec   +2 more
doaj  

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