A versatile polyacrylamide gel electrophoresis based sulfotransferase assay
BMC Biotechnology, 2010 Background Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known
Prather Brittany +4 more
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Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives
BioTechniques, 2008 The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms.
Haleem J. Issaq, Timothy D. Veenstra
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Silver Staining of Proteins in 2DE Gels [PDF]
, 2009 Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille +3 more
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Sweet silver: A formaldehyde-free silver staining using aldoses as developing agents, with enhanced compatibility with mass spectrometry [PDF]
, 2008 Protein detection methods after electrophoresis have to be sensitive, homogeneous, and not to impair downstream analysis of proteins by MS. Speed, low cost, and user friendliness are also favored features. Silver staining combines many of these features,
Berggren +26 more
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Conducting polymer electrodes for gel electrophoresis.
PLoS ONE, 2014 In nearly all cases, electrophoresis in gels is driven via the electrolysis of water at the electrodes, where the process consumes water and produces electrochemical by-products. We have previously demonstrated that π-conjugated polymers such as poly(3,4-
Katarina Bengtsson +2 more
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Membrane proteins and proteomics: Love is possible, but so difficult [PDF]
, 2009 Despite decades of extensive research, the large-scale analysis of membrane proteins remains a difficult task. This is due to the fact that membrane proteins require a carefully balanced hydrophilic and lipophilic environment, which optimum varies with ...
Adessi +103 more
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Molecular Weight Distribution of Proteins Synthesized in Single, Identified Neurons of Aplysia [PDF]
, 1971 Parietovisceral ganglia from Aplysia californica were incubated in medium containing leucine-3H. Single, identified nerve cell somas were isolated from the ganglia, and their proteins extracted and separated by electrophoresis on 5% SDS-polyacrylamide ...
Wilson, David L.
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Harčova Nauka ì Tehnologìâ, 2018 The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo +3 more
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Fractionation and characterization of euchromatin isolated from mouse ascites sarcoma cells [PDF]
, 1978 Euchromatin specimen prepared by the usual method formed large clumps and had various shapes under electron microscopy. A method of separation of the euchromatin specimen into chromatin fractions having relatively homogeneous form was examined and ...
Inaba, Kozo +2 more
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Preparative electrophoresis of human apolipoprotein E: an improved method.
Journal of Lipid Research, 1990 Apolipoprotein E was isolated from human very low density lipoproteins by a two-step electrophoretic procedure derived from that of Méndez (1982. Anal. Biochem. 126: 403-408).
S Meunier +3 more
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