Results 311 to 320 of about 523,208 (360)

Discontinuous polyacrylamide gel electrophoresis of RNA

Analytical Biochemistry, 1972
Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
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The fractionation of histones by electrophoresis in polyacrylamide gel

Analytical Biochemistry, 1965
Abstract Increased interest in histone fractionation is evidenced by the many published electrophoretic techniques using starch (1–3) and polyacrylamide (4–6) gels. Twenty-two components of calf thymus histone have been identified by Neelin and Neelin (7) with starch gel electrophoresis. Driedger et al.
G F, Vande Woude, F F, Davis
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SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
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SDS-Polyacrylamide Gel Electrophoresis of Proteins

Cold Spring Harbor Protocols, 2006
INTRODUCTIONThis protocol describes the separation of proteins by SDS-polyacrylamide gel electrophoresis. SDS is used with a reducing agent and heat to dissociate the proteins. SDS-polypeptide complexes form and migrate through the gels according to the size of the polypeptide.
Joseph, Sambrook, David W, Russell
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Separation of Colicins by Polyacrylamide Gel Electrophoresis

Journal of Applied Bacteriology, 1972
S ummary : Colicins can be separated by polyacrylamide gel electrophoresis, so allowing multiple colicinogeny to be detected with a universal indicator strain.
K G, Hardy, H C, Neimark
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SIMPLIFIED “DISC” (POLYACRYLAMIDE GEL) ELECTROPHORESIS*

Annals of the New York Academy of Sciences, 1964
SummaryCross‐linked homogeneous 5 per cent polyacrylamide gel in rod form is a convenient medium for sharp analytical electrophoretic separation in microgram quantities of most serum proteins and of many biological ampholytes.
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Polyacrylamide Temperature Gradient Gel Electrophoresis

2013
Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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Electrophoresis of DNA in Nondenaturing Polyacrylamide Gels

2003
For the majority of purposes, such as restriction mapping and sizing of cloned fragments, electrophoresis of DNA in horizontal agarose gels is perfectly adequate. As the size of the fragments of interest decreases to below 1000 base pairs (bp), however, the resolution deteriorates significantly, and an alternative is required.
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Preparative electrophoresis on polyacrylamide gel

Analytical Biochemistry, 1964
D, RACUSEN, N, CALVANICO
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Neutral Polyacrylamide Gel Electrophoresis

Cold Spring Harbor Protocols, 2006
Joseph, Sambrook, David W, Russell
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