Results 331 to 340 of about 722,396 (378)

Polyacrylamide Gel Electrophoresis

Springer Protocols Handbooks, 2022
Kamal Gandhi, Neelima Sharma, P. Gautam, Rajan Sharma, B. Mann, V. Pandey   +5 more
semanticscholar   +1 more source

Fractionation of oligodeoxynucleotides by polyacrylamide gel electrophoresis

Analytical Biochemistry, 1969
Abstract Polyacrylamide gel electrophoresis has been used to fractionate mixtures of oligomers produced by enzymic digestion of dAT copolymer with pancreatic DNase I. Fractions with degrees of polymerization from about 6 to beyond 40 were obtained with high resolution.
Elliot L. Elson, Thomas M. Jovin
openaire   +3 more sources

Discontinuous polyacrylamide gel electrophoresis of RNA

Analytical Biochemistry, 1972
Abstract A discontinuous polyacrylamide gel system is described which allows the simultaneous resolution of 38S, 28S, 18S, 5S, and 4S RNA molecules from total RNA extracts on a single gel. The gels can be constructed easily and reproducibly without special apparatus. Also reported is the use of an octadecene solution to facilitate overlayering of low
openaire   +3 more sources

SDS – polyacrylamide gel electrophoresis of lipopolysaccharides

Canadian Journal of Microbiology, 1975
Lipopolysaccharides (LPS) prepared from four different species of Neisseria have been separated by SDS – polyacrylamide gel electrophoresis. Each LPS possessed a characteristic mobility on gels. Examination of the effect of acrylamide concentration on migration illustrated that the basis of the separation was molecular size, and not intrinsic charge.
K. G. Johnson, R. R. B. Russell
openaire   +3 more sources

A miniaturized system for electrophoresis on polyacrylamide gels

Analytical Biochemistry, 1979
The design, construction, and use of a simple economical system for gel electrophoresis are described, along with effective procedures and the required materials. Examples of successful use of the system are presented.
Clement L. Markert, Zen-ichi Ogita
openaire   +3 more sources

Electrophoresis of polysaccharides in a polyacrylamide gel

Bulletin of the Academy of Sciences of the USSR Division of Chemical Science, 1969
A metod for the electrophoresis of acid and neutral polysaccharides in polyacrylamide gel is described.
A. F. Pavlenko, Yu. S. Ovodov
openaire   +2 more sources

Disc Electrophoresis in Polyacrylamide Gel

1979
Earlier electrophoretic procedures, such as paper electrophoresis described in Exercise No. 9 of this Manual, were rapidly replaced by the much better resolving electrophoresis procedures in gel. While regular paper or cellulose acetate paper electrophoresis detected a maximum of 6 to 7 components in a human serum sample, electrophoresis in ...
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Polyacrylamide Temperature Gradient Gel Electrophoresis

2013
Temperature Gradient Gel Electrophoresis (TGGE) is a form of electrophoresis in which temperature gradient is used to denature molecules as they move through either acrylamide or agarose gel. TGGE can be applied to analyze DNA, RNA, protein-DNA complexes, and, less commonly, proteins.
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Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa.

Analytical Biochemistry, 1987
Gger, And, Gebhard, von Jagow
semanticscholar   +1 more source

Gradient SDS Polyacrylamide Gel Electrophoresis

2003
The preparation of fixed-concentration polyacrylamide gels has been described in Chapter 6. However, the use of polyacrylamide gels that have a gradient of increasing acrylamide concentration (and hence decreasing pore size) can sometimes have advantages over fixed-concentration acrylamide gels. During electrophoresis in gradient gels, proteins migrate
openaire   +2 more sources