Results 41 to 50 of about 509,057 (336)
Overexpression of CHRDL2 in colon cancer cells makes them more stem‐like and resistant to chemo‐ and radiotherapy. CHRDL2‐high cells have upregulation of the WNT pathway, genes involved in the DNA damage response (DDR) pathway and epithelial‐to‐mesenchymal transition (EMT). This leads to quicker repair of damaged DNA and more cell migration.
Eloise Clarkson, Annabelle Lewis
wiley +1 more source
Electrophoretic separation of proteins [PDF]
There are several structural and storage proteins in the tissues of Crustacea. Some of them are metallo-proteins, some are enzymic in nature. Several electrophoretic procedures were used for the separation of these proteins.
Ravindranath, M H, Subhashini, M H
core
Two-dimensional gel electrophoresis in proteomics: A tutorial [PDF]
Two-dimensional electrophoresis of proteins has preceded, and accompanied, the birth of proteomics. Although it is no longer the only experimental scheme used in modern proteomics, it still has distinct features and advantages.
##Righetti+108 more
core +3 more sources
SDS-polyacrylamide electrophoretic patterns of Chlamydomonas flagellum membrane proteins displayad 6 fractions, 3 PAS-positive among them. The surface radiolabeling of the flagellum membrane suggested an outer surface exposure of fraction '5', and ...
Aleksander F. Sikorski
doaj +1 more source
Advanced glycation end products promote the release of endothelial cell‐derived mitocytosis
Under diabetic conditions, AGEs induce mitochondrial damage in HUVECs, activating migrasome‐mediated mitocytosis. Migrasomes encapsulate damaged mitochondria and are released into the extracellular space, facilitating intercellular mitochondrial transfer.
Rong Liu+6 more
wiley +1 more source
Silver Staining of Proteins in 2DE Gels [PDF]
Silver staining detects proteins after electrophoretic separation on polyacrylamide gels. Its main positive features are its excellent sensitivity (in the low nanogram range) and the use of very simple and cheap equipment and chemicals.
Chevallet, Mireille+3 more
core +4 more sources
Detection of rotavirus genome by new silver staining method
Background and Objective: Rotaviruses are the members of the Reoviridae family containing double-stranded RNA (dsRNA) genome which are the main cause of gastroinentritis particularly in children less than three years.
Ahmadi E, Soleimanjahi H, Teimoori A
doaj
Blue-native PAGE in plants: a tool in analysis of protein-protein interactions
Intact protein complexes can be separated by apparent molecular mass using a standard polyacrylamide gel electrophoresis system combining mild detergents and the dye Coomassie Blue.
Millar A Harvey+2 more
doaj +1 more source
A simple method of drying polyacrylamide slab gels that eliminates cracking
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
doaj +1 more source
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna+4 more
wiley +1 more source