Results 41 to 50 of about 693,927 (396)
Purification and characterization of putrescine synthase from cucumber seedlings. A multifunctional enzyme involved in putrescine biosynthesis [PDF]
, 1986 The multifunctional enzyme, putrescine synthase has been purified fromCucumis sativus and characterized. This enzyme harbours agmatine iminohydrolase, ornithine transcarbamylase, putrescine transcarbamylase and carbamate kinase activities, whose ...
Adiga, P. R., Prasad, G. L.
core +1 more source
Polyacrylamide Gel Electrophoresis of Plasteins
Agricultural and Biological Chemistry, 1974 A peptic hydrolysate of soybean protein was filtered with Sephadex G–25 and was separated approximately into four fractions (I, II, II, and IV in the order of mol. wt.). Fraction II (av. mol. wt: 1043) and III (av. mol. Wt.: 685) were more plastein-productive than others.
Soichi Arai +3 more
openaire +3 more sources
Development of Pipetting Devices to Separate Protein Complexes [PDF]
arXiv, 2022 The objective of this project is to develop an automated device used to spot protein samples on a hydrophobic membrane to be used for the patented electrophoresis method developed by Chang and Yonan in 2008 [1]. This novel method performs electrophoresis directly on hydrophobic blot membranes as opposed to the previous popular methods such as the 2-D ...
arxiv
An Analysis System for DNA Gel Electrophoresis Images Based on Automatic Thresholding an Enhancement [PDF]
IEEE Electro/Information Technology, 2007, 2016 Gel electrophoresis, a widely used technique to separate DNA according to their size and weight, generates images that can be analyzed automatically. Manual or semiautomatic image processing presents a bottleneck for further development and leads to reproducibility issues.
arxiv +1 more source
Harčova Nauka ì Tehnologìâ, 2018 The article considers the possibility of obtaining purified fractions-precursors of bioactive peptides from milk proteins by the method of preparative electrophoresis.
V. Yukalo +3 more
doaj +1 more source
Analysis of chitosan molecular weight profile by electrophoresis in a porosity step gradient polyacrylamide gel [PDF]
The Ukrainian Biochemical Journal, 2022 Chitosan is biocompatible and biodegradable natural biopolymer widely applied in various fields of biology, medicine, and pharmacy, however, its effects significantly depend on the degree of polymerization (DP) and the degree of deacetylation (DDA) of ...
N. O. Manko +3 more
doaj +1 more source
Proceedings of the National Academy of Sciences of the United States of America, 1989 We developed mobility shift analysis of single-stranded DNAs on neutral polyacrylamide gel electrophoresis to detect DNA polymorphisms. This method follows digestion of genomic DNA with restriction endonucleases, denaturation in alkaline solution, and ...
Masato Orita +4 more
semanticscholar +1 more source
Static Virus Spread Algorithm for DNA Sequence Design [PDF]
arXiv, 2023 DNA is not only the genetic material of life, but also a favorable material for a new computing model. Various research works based on DNA computing have been carried out in recent years. DNA sequence design is the foundation of such research. The sequence quality directly affects the universality, robustness, and stability of DNA computing.
arxiv
Silver staining of proteins in polyacrylamide gels [PDF]
Nat Protoc 1, 4 (2006) 1852-8, 2007 Silver staining is used to detect proteins after electrophoretic separation on polyacrylamide gels. It combines excellent sensitivity (in the low nanogram range) with the use of very simple and cheap equipment and chemicals. It is compatible with downstream processing, such as mass spectrometry analysis after protein digestion. The sequential phases of
arxiv +1 more source
Türk Biyokimya Dergisi, 2022 In this study, we aimed to compare modified Krauss polyacrylamide gradient gel electrophoresis (PAGGE) and high-performance liquid chromatography (HPLC) methods in classification, quantification, and separation of lipoproteins and determining low-density
Cihan Murat +3 more
doaj +1 more source