Results 21 to 30 of about 529,599 (321)

Synthesis and Characterization of Polyethylene Glycol-Grafted Photoreactive Polyethylene Glycols for Antibiofouling Applications

open access: yesPolymers, 2022
Notably, antibiofouling is an important and predominant technique adopted to improve the surfaces of biomaterials. In this study, polyethylene glycol-grafted polyethylene glycols bearing azidophenyl groups were synthesized and immobilized on polystyrene surfaces via photoirradiation.
Mahmoud H. Othman   +2 more
openaire   +2 more sources

Revealing protein structures: crystallization of protein‐ligand complexes – co‐crystallization and crystal soaking

open access: yesFEBS Open Bio, Volume 15, Issue 4, Page 542-550, April 2025.
Co‐crystallization crystallizes a protein with its ligand, resulting in protein–ligand complex crystals. In contrast, soaking introduces a ligand into preformed protein crystals, allowing it to bind. Both methods produce crystals for X‐ray diffraction, which generates diffraction patterns that are analyzed to determine the three‐dimensional structure ...
Barbora Kaščáková   +4 more
wiley   +1 more source

Atomic force microscopy (AFM) study of thick lamellar stacks of phospholipid bilayers [PDF]

open access: yesPhys. Rev. E 77, 021905 (2008) (8 pages)., 2007
We report an Atomic Force Microscopy (AFM) study on thick multi lamellar stacks of approx. 10 mum thickness (about 1500 stacked membranes) of DMPC (1,2-dimyristoyl-sn-glycero-3-phoshatidylcholine) deposited on silicon wafers. These thick stacks could be stabilized for measurements under excess water or solution.
arxiv   +1 more source

Protein crystallization and structure determination at room temperature in the CrystalChip

open access: yesFEBS Open Bio, Volume 15, Issue 4, Page 532-541, April 2025.
This protocol describes the implementation of a simple and user‐friendly microfluidic device called CrystalChip that allows the production of crystals by the counter‐diffusion method and their in situ analysis by serial crystallography. As an illustration, it is applied to determine the 3D structure of three proteins from data collected at room ...
Petr Pachl   +9 more
wiley   +1 more source

Microscale adhesion patterns for the precise localization of amoeba [PDF]

open access: yes, 2009
In order to get a better understanding of amoeba-substrate interactions in the processes of cellular adhesion and directional movement, we engineered glass surfaces with defined local adhesion characteristics at a micrometric scale. Amoeba (Dictyostelium dicoideum) is capable to adhere to various surfaces independently of the presence of extracellular ...
arxiv   +1 more source

Artificial Receptor in Synthetic Cells Performs Transmembrane Activation of Proteolysis

open access: yesAdvanced Biology, Volume 9, Issue 5, May 2025.
Transmembrane signaling is the hallmark of living cells and is among the highest challenges for the design of synthetic cells. Herein, an artificial receptor based on the chemistry of self‐immolative linkers is used to communicate information across the lipid bilayer, for transmembrane activation of enzymatic activity. Abstract The design of artificial,
Ane Bretschneider Søgaard   +7 more
wiley   +1 more source

Mucus Physically Restricts Influenza A Viral Particle Access to the Epithelium

open access: yesAdvanced Biology, Volume 9, Issue 4, April 2025.
The infectious potential of respiratory viruses depends on their ability to navigate through a layer of mucus that serves as a protective barrier in the lung. It is demonstrated that influenza A virus penetration through mucus is significantly limited by the gel's internal microstructure and, to a lesser extent, by adhesive binding to mucin glycans ...
Logan Kaler   +8 more
wiley   +1 more source

Distinct Network Morphologies from In Situ Polymerization of Microtubules in Giant Polymer‐Lipid Hybrid Vesicles

open access: yesAdvanced Biology, Volume 9, Issue 5, May 2025.
The successful in situ polymerization of microtubules in giant polymer‐lipid hybrid vesicles is illustrated. The microtubules formed in the giant hybrid vesicles exhibit different morphologies including lumenal network formation and membrane association. Abstract Creating artificial cells with a dynamic cytoskeleton, akin to those in living cells, is a
Paula De Dios Andres   +4 more
wiley   +1 more source

Universality in coalescence of polymeric fluid drops [PDF]

open access: yes, 2019
Coalescence of liquid droplets involves an interplay between capillary forces, viscous forces and inertial forces. Here, we unveil a universal temporal evolution of the neck radius during the coalescence of two polymeric drops. Through high speed imaging we demonstrate that drops of polyacrylamide (PAM), poly-vinyl alcohol (PVA), polyethylene oxide ...
arxiv   +1 more source

Drug delivery with carbon nanotubes for in vivo cancer treatment [PDF]

open access: yesCancer Res. 68 (2008) 6652-6660, 2008
Chemically functionalized single-walled carbon nanotubes (SWNTs) have shown promise in tumor targeted accumulation in mice and exhibit biocompatibility, excretion and little toxicity. Here, we demonstrate in-vivo SWNT drug delivery for tumor suppression in mice.
arxiv   +1 more source

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