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Suitability of polyethylene glycol for precipitation of monoclonal antibodies— a radiometric study
International Journal of Radiation Applications and Instrumentation. Part A. Applied Radiation and Isotopes, 1991125I labelled monoclonal antibody (MoAb) to thyroid stimulating hormone (TSH) was used as a tracer for assessing the suitability of polyethylene glycol (PEG) for precipitation of MoAb and MoAb-antigen (AG) complexes. It was found that PEG can be advantageously used under certain conditions favouring insolubility and could thus be a non-specific ...
A, Rathinaswamy +4 more
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Fibronectin in polyethylene glycol precipitates: evidence for a role in immune complexes
Rheumatology International, 1986Fibronectin is involved in the opsonic clearance of particulate material. It is present in plasma and synovial fluid and thus might be expected to have a role in the clearance of immune complexes. We have investigated this in a study of polyethylene glycol (PEG) precipitable material from the serum of patients with rheumatoid arthritis, systemic lupus ...
J S, Coppock +4 more
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Purification and Aggregation of Influenza Virus by Precipitation with Polyethylene Glycol
Preparative Biochemistry, 1974ABSTRACT Influenza virus may be purified and rendered free of extraneous proteins by precipitation and aggregation with polyethylene glycol at polymer concentrations of 1 to 4%. The precipitated virus is superior antigenically to the virus in monomeric and in the ether dissociated forms.
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Purification of IgG by Precipitation with Polyethylene Glycol (PEG)
1996PEG precipitation works well for IgM, but is less efficient for IgG; salt precipitation methods are usually recommended for IgG. PEG precipitation may be preferred in multistep purifications that use ion-exchange columns, because the ionic strength of the Ig is not altered.
Mark Page, Robin Thorpe
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Radioimmunoassay for triiodothyronine: use of polyethylene glycol to precipitate immune complexes
Clinical Biochemistry, 19751. A rapid radioimmunoassay (RIA) for determining serum triiodothyronine (T3) concentration has been developed. The reaction of T3 with its antibody was performed at 37 degrees C for 3 hours; precipitation of antibody bound T3 by 15% polyethylene glycol (PEG) was done at ice-bath temperatures. 2.
S L, Twomey, R, Sweat
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Limitations of Polyethylene Glycol Precipitation Assays for Circulating Immune Complexes
American Journal of Clinical Pathology, 1981Polyethylene glycol precipitation assays (PEG-A) have been promoted as technically simple, inexpensive methods for detecting and quantitating circulating immune complexes; however, their specificity and sensitivity have not been clearly defined. Data are presented showing that the percent recovery of IgG in 3.5% PEG increases as the concentration of ...
R B, Traycoff, D, Muegge, W, Rogers
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Precipitation of proteins with polyethylene glycol.
Methods in enzymology, 1990Polyethylene glycol is a nondenaturing water-soluble polymer whose ability to precipitate protein from aqueous solution can be qualitatively understood in terms of an excluded volume mechanism. The increment in PEG concentration required to effect a given reduction in solubility is unique for a given protein-polymer pair, being insensitive to solution ...
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Protein precipitation with polyethylene glycol.
Methods in enzymology, 1984Polyethylene glycol is a nondenaturing water-soluble polymer whose ability to precipitate protein from aqueous solution can be qualitatively understood in terms of an excluded volume mechanism. The increment in PEG concentration required to effect a given reduction in solubility is unique for a given protein-polymer pair, being insensitive to solution ...
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A Case of Screening Macroprolactinemia Using Polyethylene Glycol Precipitation Method
Clinical LaboratoryHyperprolactinemia is an endocrine disorder characterized by abnormally elevated levels of prolactin (PRL) in the serum. Macroprolactinemia refers to the condition where more than 60% of the prolactin circulating in the peripheral blood is composed of high molecular weight macroprolactins.
Gangfeng, Li, Ningping, Shan, Qian, Wang
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Prevalence of macroamylasaemia using polyethylene glycol precipitation as a screening method
Annals of Clinical Biochemistry: International Journal of Laboratory Medicine, 2001Four hundred and fifty-four subjects with hyperamylasaemia were screened for the presence of macroamylase using polyethylene glycol precipitation (PEG) and the Beckman automated amylase assay based on the hydrolysis of maltotetraose. Twenty-five subjects (5·5%) exhibited PEG precipitation values suggestive of macroamylasaemia (MA) (> 52% loss of ...
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