ABSTRACT The facultative chemolithoautotroph Cupriavidus necator is capable of heterotrophic growth on diverse carbon sources, or of autotrophic growth using CO2 fixation with H2 as an energy source. Under stress conditions, it produces biodegradable polyesters (polyhydroxyalkanoates, PHAs) as a storage material occupying a high proportion of the total
Kate McKeever +9 more
wiley +1 more source
Development of an immunoFET biosensor for the detection of biotinylated PCR product
ImmunoFET (IMFET) biosensor is a simple platform for the detection of biotinylated products of polymerase chain reaction (PCR). Construction of the IMFET biosensor started with adsorption of 1.5 mg/mL of protein A (PA) onto the insulated gate surface of ...
Wannaporn Muangsuwan +8 more
doaj +1 more source
This review covers the mechanical forces experienced by immune cells through cell–cell and cell–extracellular matrix interactions and how these forces influence their receptors and functions. These relationships between forces and cellular functions can be exploited using engineering techniques to modify the physical properties of materials for novel ...
Yu‐Chang Chen +2 more
wiley +1 more source
Improved Properties of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Produced by Comamonas sp. EB172 Utilizing Volatile Fatty Acids by Regulating the Nitrogen Source [PDF]
This study presents the effect of carbon to nitrogen ratio (C/N) (mol/mol) on the cell growth and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) accumulation by Comamonas sp.
Abd-Aziz Suraini +4 more
core +2 more sources
Poly(3-hydroxyalkanoate) polymerase synthesis and in vitro activity in recombinant Escherichia coli and Pseudomonas putida [PDF]
We tested the synthesis and in vitro activity of the poly(3-hydroxyalkanoate) (PHA) polymerase 1 from Pseudomonas putida GPo1 in both P. putida GPp104 and Escherichia coli JMU193. The polymerase encoding gene phaC1 was expressed using the inducible PalkB
de Roo, Guy +5 more
core
Fatty acid synthesis in Escherichia coli and its applications towards the production of fatty acid based biofuels [PDF]
The idea of renewable and regenerative resources has inspired research for more than a hundred years. Ideally, the only spent energy will replenish itself, like plant material, sunlight, thermal energy or wind.
Janßen, H. (Helge) +1 more
core +1 more source
Polyhydroxyalkanoate (PHA) Biosynthesis From Local Mixed Cultures And Burkholderia [PDF]
Polyhydroxyalkanoate (PHA) production was conducted by mixed cultures obtained from various niches (rubber plantation soil, oil palm plantation soil, industrial aeration tank, industrial sedimentation tank, oil polluted wastewater and lake water) in ...
Chee, Jiun Yee
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Investigations on the microbial catabolism of the organic sulfur compounds TDP and DTDP in H16 employing DNA microarrays [PDF]
In this study, we have investigated the transcriptome of Ralstonia eutropha H16 during cultivation with gluconate in presence of 3,3'-thiodipropionic acid (TDP) or 3,3'-dithiodipropionic acid (DTDP) during biosynthesis of poly(3-hydroxybutyrate-co-3 ...
Alexander Steinbüchel +4 more
core +2 more sources
Aeromonas hydrophila CGMCC 0911 possessing type I polyhydroxyalkanoate (PHA) synthase (PhaC) produced only PHBHHx from lauric acid but not from glucose. Medium-chain-length (mcl) PHA was produced from lauric acid or glucose only when PhaC of A. hydrophila was inactivated, indicating the existence of another PHA synthase in the wild type.
Fengqing, Hu, Song, You
openaire +2 more sources
Novel Polyhydroxyalkanoate blends: their characterisation and possible applications [PDF]
Polyhydroxyalkanoates (PHAs) are polyesters consisting of 3-hydroxyalkanoic acids synthesised by numerous bacteria as storage compounds, in the presence of excess carbon, under nutrient limiting conditions.
Kanjirathumutil, C.T. +1 more
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