Results 11 to 20 of about 2,158,859 (241)
Are There Mutator Polymerases? [PDF]
The Scientific World JOURNAL, 2003 DNA polymerases are involved in different cellular events, including genome replication and DNA repair. In the last few years, a large number of novel DNA polymerases have been discovered, and the biochemical analysis of their properties has revealed a long list of intriguing features.
García Díaz, Miguel +4 more
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Bst polymerase — a humble relative of Taq polymerase
Computational and Structural Biotechnology Journal, 2023 DNA polymerases are a superfamily of enzymes synthesizing DNA using DNA as a template. They are essential for nucleic acid metabolism and for DNA replication and repair. Modern biotechnology and molecular diagnostics rely heavily on DNA polymerases in analyzing nucleic acids.
Igor Oscorbin, Maxim Filipenko
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International Journal of Molecular Sciences, 2019 Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair. Interestingly, all eukaryotes possess a large repertoire of polymerases.
Pedroza-Garcia, Jose-Antonio +2 more
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DNA polymerases in Biotechnology [PDF]
Frontiers in Microbiology, 2014 DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology.
Gardner, Andrew F., Kelman, Zvi
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RNA Polymerases of Maize: Nuclear RNA Polymerases [PDF]
Proceedings of the National Academy of Sciences, 1971 Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays . The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH 4 ) 2 SO 4 , respectively.
Kathleen P. Mullinix +2 more
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DNA Polymerase e - More Than a Polymerase [PDF]
The Scientific World JOURNAL, 2003 This paper presents a comprehensive review of the structure and function of DNA polymerase e. Together with DNA polymerases a and d, this enzyme replicates the nuclear DNA in the eukaryotic cell. During this process, DNA polymerase a lays down RNA-DNA primers that are utilized by DNA polymerases d and e for the bulk DNA synthesis.
Helmut Pospiech, Juhani E. Syväoja
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Characteristics of Pan-Cancer Patients With Ultrahigh Tumor Mutation Burden
Frontiers in Oncology, 2021 BackgroundTumor mutation burden has been proven to be a good predictor for the efficacy of immunotherapy, especially in patients with hypermutation. However, most research focused on the analysis of hypermutation in individual tumors, and there is a lack
Hong Yuan +13 more
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Beyond the Lesion: Back to High Fidelity DNA Synthesis
Frontiers in Molecular Biosciences, 2022 High fidelity (HiFi) DNA polymerases (Pols) perform the bulk of DNA synthesis required to duplicate genomes in all forms of life. Their structural features, enzymatic mechanisms, and inherent properties are well-described over several decades of research.
Joseph D. Kaszubowski +1 more
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Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]
, 2017 In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M +9 more
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Reconstitution of recombination-associated DNA synthesis with human proteins. [PDF]
, 2013 The repair of DNA breaks by homologous recombination is a high-fidelity process, necessary for the maintenance of genome integrity. Thus, DNA synthesis associated with recombinational repair must be largely error-free.
Grossi, Sara M +4 more
core +1 more source