Results 21 to 30 of about 1,891,612 (343)

Structure of replicating SARS-CoV-2 polymerase

Nature, 2020
The new coronavirus severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) uses an RNA-dependent RNA polymerase (RdRp) for the replication of its genome and the transcription of its genes1–3.
H. S. Hillen, G. Kokic, L. Farnung, C. Dienemann, D. Tegunov, P. Cramer   +5 more
semanticscholar   +1 more source

Chicken eEF1α is a Critical Factor for the Polymerase Complex Activity of Very Virulent Infectious Bursal Disease Virus

Viruses, 2020
Infectious bursal disease (IBD) is an immunosuppressive, highly contagious, and lethal disease of young chickens caused by IBD virus (IBDV). It results in huge economic loss to the poultry industry worldwide.
Bo Yang, Nana Yan, Aijing Liu, Yue Li, Zehua Chen, Li Gao, Xiaole Qi, Yulong Gao, Changjun Liu, Yanping Zhang, Hongyu Cui, Kai Li, Qing Pan, Yongqiang Wang, Xiaomei Wang   +14 more
doaj   +1 more source

Recombinant GII.Pe-GII.4 Norovirus, Thailand, 2017–2018

Emerging Infectious Diseases, 2019
During June 2017–December 2018, norovirus was responsible for 10.9% of acute gastroenteritis cases in Thailand. Genogroup I (GI) was found in 14% of samples, of which 12 were co-infected with genogroup II (GII).
Watchaporn Chuchaona, Jira Chansaenroj, Nasamon Wanlapakorn, Sompong Vongpunsawad, Yong Poovorawan   +4 more
doaj   +1 more source

Viral Polymerases [PDF]

, 2011
Viral polymerases play a central role in viral genome replication and transcription. Based on the genome type and the specific needs of particular virus, RNA-dependent RNA polymerase, RNA-dependent DNA polymerase, DNA-dependent RNA polymerase, and DNA-dependent RNA polymerases are found in various viruses.
openaire   +2 more sources

Transitioning to DNA genomes in an RNA world

eLife, 2017
The unexpected ability of an RNA polymerase ribozyme to copy RNA into DNA has ramifications for understanding how DNA genomes evolved.
Razvan Cojocaru, Peter J Unrau
doaj   +1 more source

The Functional Implications of Broad Spectrum Bioactive Compounds Targeting RNA-Dependent RNA Polymerase (RdRp) in the Context of the COVID-19 Pandemic

Viruses, 2023
Background: As long as COVID-19 endures, viral surface proteins will keep changing and new viral strains will emerge, rendering prior vaccines and treatments decreasingly effective.
Brittany A. Comunale, Robin J. Larson, Erin Jackson-Ward, Aditi Singh, Frances L. Koback, Lilly D. Engineer   +5 more
doaj   +1 more source

The R251K Substitution in Viral Protein PB2 Increases Viral Replication and Pathogenicity of Eurasian Avian-like H1N1 Swine Influenza Viruses

Viruses, 2020
The Eurasian avian-like swine (EA) H1N1 virus has affected the Chinese swine industry, and human infection cases have been reported occasionally. However, little is known about the pathogenic mechanism of EA H1N1 virus.
Mengkai Cai, Ruting Zhong, Chenxiao Qin, Zhiqing Yu, Xiaoyan Wen, Junsi Xian, Yongjie Chen, Yu Cai, Heyou Yi, Lang Gong, Guihong Zhang   +10 more
doaj   +1 more source

Polymerase Chain Reaction [PDF]

Journal of Investigative Dermatology, 2013
The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project.
Garibyan, Lilit, Avashia, Nidhi
openaire   +2 more sources

Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors

Frontiers in Bioengineering and Biotechnology
We present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev, Zhian Zhang, Wayne M. Barnes, Wayne M. Barnes   +3 more
doaj   +1 more source

Biochemistry of the Respiratory Syncytial Virus L Protein Embedding RNA Polymerase and Capping Activities

Viruses, 2023
The human respiratory syncytial virus (RSV) is a negative-sense, single-stranded RNA virus. It is the major cause of severe acute lower respiratory tract infection in infants, the elderly population, and immunocompromised individuals.
Priscila Sutto-Ortiz, Jean-François Eléouët, François Ferron, Etienne Decroly   +3 more
doaj   +1 more source