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International Journal of Molecular Sciences, 2019 Maintenance of genome integrity is a key process in all organisms. DNA polymerases (Pols) are central players in this process as they are in charge of the faithful reproduction of the genetic information, as well as of DNA repair. Interestingly, all eukaryotes possess a large repertoire of polymerases.
Pedroza-Garcia, Jose-Antonio +2 more
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Mobile genetic element-encoded putative DNA primases composed of A-family polymerase—SSB pairs
Frontiers in Molecular Biosciences, 2023 Mobile genetic elements can encode a wide variety of genes that support their own stability and mobility as well as genes that provide accessory functions to their hosts.
Phoebe A. Rice
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DNA polymerases in Biotechnology [PDF]
Frontiers in Microbiology, 2014 DNA polymerases are core tools for molecular biology including PCR, whole genome amplification, DNA sequencing and genotyping. Research has focused on discovery of novel DNA polymerases, characterization of DNA polymerase biochemistry and development of new replication assays. These studies have accelerated DNA polymerase engineering for biotechnology.
Gardner, Andrew F., Kelman, Zvi
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Журнал микробиологии, эпидемиологии и иммунобиологии, 2023 Introduction. The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) possesses 5'-3' DNA polymerase activity, 5'-3' displacement activity and high processivity.
Maria I. Pika +9 more
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Open Biology, 2023 The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the Enterobacteriaceae. ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase ...
Miriam Weckener +8 more
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RNA Polymerases of Maize: Nuclear RNA Polymerases [PDF]
Proceedings of the National Academy of Sciences, 1971 Two DNA-dependent RNA polymerases of nuclear origin have been purified from leaves of Zea mays . The two enzymes can be separated on DEAE-cellulose columns. Enzymes I and II are eluted with 0.08 and 0.20 M (NH 4 ) 2 SO 4 , respectively.
Kathleen P. Mullinix +2 more
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DNA Polymerase e - More Than a Polymerase [PDF]
The Scientific World JOURNAL, 2003 This paper presents a comprehensive review of the structure and function of DNA polymerase e. Together with DNA polymerases a and d, this enzyme replicates the nuclear DNA in the eukaryotic cell. During this process, DNA polymerase a lays down RNA-DNA primers that are utilized by DNA polymerases d and e for the bulk DNA synthesis.
Helmut Pospiech, Juhani E. Syväoja
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Transcription-translation coupling: direct interactions of RNA polymerase with ribosomes and ribosomal subunits. [PDF]
, 2017 In prokaryotes, RNA polymerase and ribosomes can bind concurrently to the same RNA transcript, leading to the functional coupling of transcription and translation.
Blaha, Gregor M +9 more
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DNA polymerase of mitochondria is a gamma-polymerase.
Journal of Biological Chemistry, 1977 Mitochondria isolated from rat liver cells or mycoplasma-free HeLa cells contain a single DNA polymerase activity which is closely related to, or identical to, the DNA polymerase gamma activity found in the homologous cell. In rat liver cells, about 16% of the total cytoplasmic gamma-polymerase activity is found associated with mitochondria and in HeLa
G P Noy, A Bolden, Arthur Weissbach
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Microfluidic polymerase chain reaction [PDF]
, 2008 We implement microfluidic technology to miniaturize a thermal cycling system for amplifying DNA fragments. By using a microfluidic thermal heat exchanger to cool a Peltier junction, we have demonstrated rapid heating and cooling of small volumes of ...
Gomez, Alvaro +4 more
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