Results 291 to 300 of about 1,891,612 (343)
Some of the next articles are maybe not open access.
Current Protocols in Molecular Biology, 2008
AbstractThis unit describes DNA‐dependent, RNA‐dependent, and template‐independent RNA polymerases. DNA‐dependent RNA polymerases include the related bacteriophage T7, T3, and SP6 polymerases, the most commonly used RNA polymerases for in vitro transcription reactions. Reaction conditions to produce preparative quantities of transcribed RNA and labeled
Beth M, Paschal +3 more
openaire +2 more sources
AbstractThis unit describes DNA‐dependent, RNA‐dependent, and template‐independent RNA polymerases. DNA‐dependent RNA polymerases include the related bacteriophage T7, T3, and SP6 polymerases, the most commonly used RNA polymerases for in vitro transcription reactions. Reaction conditions to produce preparative quantities of transcribed RNA and labeled
Beth M, Paschal +3 more
openaire +2 more sources
Annual Review of Biochemistry, 2002
▪ Abstract Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during ...
Hubscher U, Maga G, Spadari S
openaire +4 more sources
▪ Abstract Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during ...
Hubscher U, Maga G, Spadari S
openaire +4 more sources
Nature, 1970
A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
openaire +3 more sources
A membrane associated DNA polymerizing enzyme has been solubilized, partially isolated and characterized.
openaire +3 more sources
Nature, 1974
DNA polymerase has been isolated from the cytoplasm of several mammalian tissues as well as several cell lines in culture1–8. This has been puzzling because DNA replication and repair are processes generally believed to be restricted to the nucleus, where chromatin is located.
J J, Byrnes +3 more
openaire +2 more sources
DNA polymerase has been isolated from the cytoplasm of several mammalian tissues as well as several cell lines in culture1–8. This has been puzzling because DNA replication and repair are processes generally believed to be restricted to the nucleus, where chromatin is located.
J J, Byrnes +3 more
openaire +2 more sources
Current Opinion in Structural Biology, 2018
The eukaryotic DNA replication machinery is conserved from yeast to humans and requires the actions of multiple DNA polymerases. In addition to replicative DNA polymerases for duplication of the leading and lagging DNA strands, another group of specialized polymerases is required for DNA repair and/or translesion DNA synthesis (TLS).
Rinku, Jain +2 more
openaire +2 more sources
The eukaryotic DNA replication machinery is conserved from yeast to humans and requires the actions of multiple DNA polymerases. In addition to replicative DNA polymerases for duplication of the leading and lagging DNA strands, another group of specialized polymerases is required for DNA repair and/or translesion DNA synthesis (TLS).
Rinku, Jain +2 more
openaire +2 more sources
Critical Reviews in Biochemistry, 1988
Recent progress in molecular biological techniques revealed that genomes of animal viruses are complex in structure, for example, with respect to the chemical nature (DNA or RNA), strandedness (double or single), genetic sense (positive or negative), circularity (circle or linear), and so on.
A, Ishihama, K, Nagata
openaire +2 more sources
Recent progress in molecular biological techniques revealed that genomes of animal viruses are complex in structure, for example, with respect to the chemical nature (DNA or RNA), strandedness (double or single), genetic sense (positive or negative), circularity (circle or linear), and so on.
A, Ishihama, K, Nagata
openaire +2 more sources
2012
DNA polymerase ε (Pol ε) is one of three replicative DNA polymerases in eukaryotic cells. Pol ε is a multi-subunit DNA polymerase with many functions. For example, recent studies in yeast have suggested that Pol ε is essential during the initiation of DNA replication and also participates during leading strand synthesis.
Matthew, Hogg, Erik, Johansson
openaire +2 more sources
DNA polymerase ε (Pol ε) is one of three replicative DNA polymerases in eukaryotic cells. Pol ε is a multi-subunit DNA polymerase with many functions. For example, recent studies in yeast have suggested that Pol ε is essential during the initiation of DNA replication and also participates during leading strand synthesis.
Matthew, Hogg, Erik, Johansson
openaire +2 more sources
Molecular Biology, 2005
Plastids have a very interesting transcription apparatus that gives us an opportunity to investigate mono- and multisubunut RNA polymerase interaction under conditions of complex biogenesis of the organelles and the necessity to coordinate the expression of genes located in different cell compartments.
E A, Lysenko, V V, Kuznetsov
openaire +2 more sources
Plastids have a very interesting transcription apparatus that gives us an opportunity to investigate mono- and multisubunut RNA polymerase interaction under conditions of complex biogenesis of the organelles and the necessity to coordinate the expression of genes located in different cell compartments.
E A, Lysenko, V V, Kuznetsov
openaire +2 more sources
Annual Review of Biochemistry, 1977
PERSPECTIVES A ND SU MMARY ......... 25 IN TR ODUCTION ........ ... 27 CE LLU LAR DNA POL YMERASES ....... . 28 DNA Polymerase a ......... 28 DNA Polymerase /3 31 DNA Polymerase 'Y •.....• • 33 Mitochondrial DNA Polymerase .... ......... 34 VIR US-INDUCED DNA POL YMERASES ......... ....
openaire +2 more sources
PERSPECTIVES A ND SU MMARY ......... 25 IN TR ODUCTION ........ ... 27 CE LLU LAR DNA POL YMERASES ....... . 28 DNA Polymerase a ......... 28 DNA Polymerase /3 31 DNA Polymerase 'Y •.....• • 33 Mitochondrial DNA Polymerase .... ......... 34 VIR US-INDUCED DNA POL YMERASES ......... ....
openaire +2 more sources
Current Opinion in Structural Biology, 2001
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
openaire +2 more sources
The recently determined crystal structure of a bacterial core RNA polymerase (RNAP) provides the first glimpse of this family of evolutionarily conserved cellular RNAPs. Using the structure as a framework, a consistent picture of protein-nucleic acid interactions in transcription complexes has been accumulated from cross-linking experiments.
openaire +2 more sources