Results 301 to 310 of about 1,891,612 (343)
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Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.
Science, 1988R. Saiki +7 more
semanticscholar +1 more source
2003
Abstract Bacteriophage T7 RNA polymerase (RNAP) is the best characterized member of a widespread family of RNAPs that includes most bacteriophage-encoded RNAPs as well as the mitochondrial RNAPs. The robust activity and strict promoter specificity of the phage RNAPs have made them extremely useful as reagents for both in vitro preparation of ...
Rui, Sousa, Srabani, Mukherjee
openaire +2 more sources
Abstract Bacteriophage T7 RNA polymerase (RNAP) is the best characterized member of a widespread family of RNAPs that includes most bacteriophage-encoded RNAPs as well as the mitochondrial RNAPs. The robust activity and strict promoter specificity of the phage RNAPs have made them extremely useful as reagents for both in vitro preparation of ...
Rui, Sousa, Srabani, Mukherjee
openaire +2 more sources
1973
Publisher Summary This chapter presents the status of RNA polymerases involved in RNA synthesis in mammalian cells, with emphasis on some recent developments in the field of nuclear RNA polymerase. The chapter discusses that the RNA polymerases of eukaryotic nuclei exist in multiple forms. These enzymes are compartmentalized within the nucleus.
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Publisher Summary This chapter presents the status of RNA polymerases involved in RNA synthesis in mammalian cells, with emphasis on some recent developments in the field of nuclear RNA polymerase. The chapter discusses that the RNA polymerases of eukaryotic nuclei exist in multiple forms. These enzymes are compartmentalized within the nucleus.
openaire +2 more sources
Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
Gene, 1989Steffan N. Hoa +4 more
semanticscholar +1 more source
Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction.
Science, 1992Peng Liang, A. Pardee
semanticscholar +1 more source
2004
At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
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At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
openaire +1 more source
Biochemical and Biophysical Research Communications, 1965
Abstract An experiment will be described which shows that DNA polymerase helps select the base in DNA replication. After elucidating the structure of DNA (1953a), Watson and Crick proposed a template mechanism for the replication of DNA (1953b).
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Abstract An experiment will be described which shows that DNA polymerase helps select the base in DNA replication. After elucidating the structure of DNA (1953a), Watson and Crick proposed a template mechanism for the replication of DNA (1953b).
openaire +2 more sources
Use of T7 RNA polymerase to direct expression of cloned genes.
Methods in Enzymology, 1990F. Studier +3 more
semanticscholar +1 more source