Results 101 to 110 of about 1,452,270 (349)

Genetic diversity in sacaca (Croton cajucara Benth.) accessed by RAPD markers. [PDF]

open access: yes, 2019
Collection and maintenance. DNA extraction, purification and quantification. PCR (polymerase chain reaction) conditions and RAPD (random amplified polymorphic DNA) band patterns documentation and ...
ANGELO, P. C. da S.   +5 more
core  

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

open access: yesFEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito   +3 more
wiley   +1 more source

A simple, rapid and economic method for detecting multidrug-resistant tuberculosis

open access: yesBrazilian Journal of Infectious Diseases, 2013
Objective: To evaluate multiplex allele specific polymerase chain reaction as a rapid molecular tool for detecting multidrug-resistant tuberculosis. Methods: Based on drug susceptibility testing, 103 isolates were multidrug-resistant tuberculosis and 45 ...
Xia Wang   +5 more
doaj   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

open access: yesFEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang   +7 more
wiley   +1 more source

DIAGNOSIS OF MALARIA BY REAL-TIME POLYMERASE CHAIN REACTION IN CASES OF NEGATIVE MALARIAL PARASITE ON MICROSCOPY

open access: yesPakistan Armed Forces Medical Journal, 2020
Objective: To determine the frequency of malaria by polymerase chain reaction in malarial parasite negative samples on microscopy. Study Design: Cross-sectional study.
Hina Mushtaq   +5 more
doaj  

Polymerase chain reaction-based gene removal from plasmids

open access: yesData in Brief, 2015
This data article contains supplementary figures and methods to the research article entitled, “Multiplex gene removal by two-step polymerase chain reactions” (Krishnamurthy et al., Anal.
Vishnu Vardhan Krishnamurthy   +4 more
doaj   +1 more source

Presence of Legionellaceae in warm water supplies and typing of strains by polymerase chain reaction [PDF]

open access: yes, 2001
Outbreaks of Legionnaire's disease present a public health challenge especially because fatal outcomes still remain frequent. The aim of this study was to describe the abundance and epidemiology of Legionellaceae in the human-made environment.
Brengelmann, F.   +3 more
core   +1 more source

FGFR Like1 drives esophageal cancer progression via EMT, PI3K/Akt, and notch signalling: insights from clinical data and next‐generation sequencing analysis

open access: yesFEBS Open Bio, EarlyView.
Clinical analysis reveals significant dysregulation of FGFRL1 in esophageal cancer (EC) patients. RNAi‐coupled next‐generation sequencing (NGS) and in vitro study reveal FGFRL1‐mediated EC progression via EMT, PI3K/Akt, and Notch pathways. Functional assays confirm its role in tumor growth, migration, and invasion.
Aprajita Srivastava   +3 more
wiley   +1 more source

Design of an Improved Polymerase Chain Reaction (PCR) Assay for Molecular Detection of Streptococcus pneumonia

open access: yesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2013
Background and Objectives: Streptococcus pneumoniae accounts for bacterial meningitis and is an important cause of morbidity among children and elderly. Control of this disease depends on rapid detection of the causative bacteria.
Fahimeh Aghamiri   +3 more
doaj   +2 more sources

Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples [PDF]

open access: yes, 2007
The determination of the presence of genetically modified plant material by the detection of expressed genetically engineered proteins using lateral flow protein strip tests has been evaluated in different matrices. The presence of five major genetically
CASI, AL   +7 more
core   +1 more source

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