Results 101 to 110 of about 1,398,764 (327)

OVERVIEW OF METHODS AND POLYMERASE-CHAIN REACTION MODELS

open access: yesMedična Informatika ta Inženerìâ, 2015
In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques ...
A. S. Sverstyuk   +2 more
doaj   +1 more source

Detection of genetically modified plant products by protein strip testing: an evaluation of real-life samples [PDF]

open access: yes, 2007
The determination of the presence of genetically modified plant material by the detection of expressed genetically engineered proteins using lateral flow protein strip tests has been evaluated in different matrices. The presence of five major genetically
CASI, AL   +7 more
core   +1 more source

Polydatin ameliorates ovalbumin‐induced asthma in a rat model through NCOA4‐mediated ferroautophagy and ferroptosis pathway

open access: yesFEBS Open Bio, EarlyView.
In this research, a rat model of asthma was created using OVA, and polydatin served as an intervention. By inhibiting ferroautophagy mediated by NCOA4 and averting ferroptosis, polydatin has been demonstrated to reduce asthma. This work presents new ideas for investigating the mechanism of polydatin's ability to alleviate asthma, in addition to ...
Wei Li   +5 more
wiley   +1 more source

Polymerase chain reaction-based gene removal from plasmids

open access: yesData in Brief, 2015
This data article contains supplementary figures and methods to the research article entitled, “Multiplex gene removal by two-step polymerase chain reactions” (Krishnamurthy et al., Anal.
Vishnu Vardhan Krishnamurthy   +4 more
doaj   +1 more source

PCR-RFLP Using BseDI Enzyme for Pork Authentication in Sausage and Nugget Products [PDF]

open access: yes, 2011
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using BseDI restriction enzyme had been applied for identifying the presence of pork in processed meat (beef sausage and chicken nugget) including before and after frying ...
Abidin, M. Z. (M)   +3 more
core  

An optimized protocol to detect high‐throughput DNA methylation from custom targeted sequences on 96 samples simultaneously

open access: yesFEBS Open Bio, EarlyView.
Workflow of a high‐throughput technology for epigenotyping of differentially methylated CpGs in specific regions of the genome. The protocol works with small amounts of DNA extracted from blood or semen. The protocol consists of both enzymatic conversion of unmethylated cytosines and capture by hybridization with a custom panel.
Nathalie Iannuccelli   +4 more
wiley   +1 more source

Polymerase Chain Reaction

open access: yesInternational Journal of Pharmacological Research, 2012
The polymerase chain reaction (PCR) is a technique in molecular biology to amplify a single or a few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of ...
Patricia Hernández-Rodríguez   +1 more
openaire   +5 more sources

Detecting beef meatball contamination with polymerase chain reaction [PDF]

open access: yesBiotika, 2017
The objective of the study was to describe how much rat and swine primer developed from cytochrome b could detect rat and pork in processed beef products sold in North Maluku.
Hoda A., Sapsuha Y., Syafie Y.
doaj  

Presence of Legionellaceae in warm water supplies and typing of strains by polymerase chain reaction [PDF]

open access: yes, 2001
Outbreaks of Legionnaire's disease present a public health challenge especially because fatal outcomes still remain frequent. The aim of this study was to describe the abundance and epidemiology of Legionellaceae in the human-made environment.
Brengelmann, F.   +3 more
core   +1 more source

Downregulation of sST2, a decoy receptor for interleukin‐33, enhances subcutaneous tumor growth in murine pancreatic cancer cells

open access: yesFEBS Open Bio, EarlyView.
Subcutaneous implantation of murine Panc02 pancreatic cancer cells depleted of sST2, a soluble decoy receptor for the proinflammatory interleukin‐33 (IL‐33), leads to a decreased number of GLUT4‐positive cancer‐associated adipocytes, reduced levels of the anti‐inflammatory molecule adiponectin, increased phosphorylation of IκBα, elevated Cxcl3 ...
Miho Akimoto   +5 more
wiley   +1 more source

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