Results 81 to 90 of about 218,617 (259)

Direct amplification and differentiation of pathogenic and nonpathogenic Entamoeba histolytica DNA from stool specimens [PDF]

, 1994
Discrimination of pathogenic and nonpathogenic Entamoeba histolytica is of great clinical importance. A simple and rapid DNA extraction method that can be used directly with stool specimens was developed without the need for prior cultivation of the ...
Katzwinkel-Wladarsch, S., Thomas Loscher, Sylvia Katzwinkel-Wladarsch, Heinz Rinder, Rinder, H., Löscher, Thomas   +5 more
core   +1 more source

Identification of Xiphinema vuittenezi by polymerase chain reaction

Plant Protection Science, 2004
So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies.
Shesh Kumari, Jiban K. Kundu, Jaroslav Polák   +2 more
doaj   +1 more source

Polymerase chain reaction in liposomes

Chemistry & Biology, 1995
Compartmentalization of biochemical reactions within a spherically closed bilayer is an important step in the molecular evolution of cells. Liposomes are the most suitable structures to model this kind of chemistry. We have used the polymerase chain reaction (PCR) to demonstrate that complex biochemical reactions such as DNA replication can be carried ...
T. OBERHOLZER, P.L. LUISI, ALBRIZIO, MARIA   +2 more
openaire   +3 more sources

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

Numerical and experimental study of a droplet-based PCR chip

, 2007
A two-temperature continuous-flow polymerase chain reaction (PCR) polymer chip has been constructed that takes advantage of droplet technology to avoid sample contamination and adsorption at the surface.
MacAskill, Alexandra, Goddard, N.J., Emerson, David, Macaskill A, Emerson DR, Day, P. J R, Fielden, P. R., Emerson, D. R., Zhang, Y. H., Mohr S, Day, Philip; id_orcid, Day PJR, Barber RW, Fielden, P.R., Barber, R. W., Zhang YH, Zhang, Yonghao, Goddard NJ, Goddard, N. J., Barber, Robert W., Macaskill, A., Mohr, S., Fielden PR, Day, P.J.R.   +23 more
core   +1 more source

Feasibility of rapid polymerase chain reaction for detection of methicillin-resistant Staphylococcus aureus colonization among emergency department patients with abscesses

Open Access Emergency Medicine, 2013
Michael S Pulia,1 Mary Calderone,2 Brad Hansen,2 Christine E Stake,3 Mark Cichon,3 Zhanhai Li,4 Nasia Safdar51Division of Emergency Medicine, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA; 2Loyola University Stritch ...
Pulia MS, Calderone M, Hansen B, Stake CE, Cichon M, Li Z, Safdar N   +6 more
doaj  

Clinical performance of the urine‐based TERT promoter AbsoluteQ Digital PCR for non‐invasive detection of bladder cancer

Molecular Oncology, EarlyView.
A urine‐based digital PCR assay targeting two hotspot TERT promoter variants detected bladder cancer with high sensitivity and no false positives in this case–control cohort. The streamlined AbsoluteQ workflow outperformed Sanger sequencing and supports non‐invasive molecular testing for bladder cancer detection.
Anna Nykel, Izabela Kubiak, Lena Rutkowska, Żaneta Kasprzyk, Łukasz Kępczyński, Michał Bednarek, Dariusz Sobieraj, Jacek Wilkosz, Piotr Kania, Adam Jędrzejczyk, Bogdan Kałużewski, Agnieszka Gach, Tadeusz Kałużewski   +12 more
wiley   +1 more source

Detection of parvovirus B19 in donated blood:a model system for screening by polymerase chain reaction

, 1993
A highly sensitive and rapid method for routinely screening large numbers of donated blood units for parvovirus B19 by the polymerase chain reaction (PCR) was developed.
Jordan, A, Cohen, B J, McOmish, F, Hart, H, Yap, P L, Simmonds, P   +5 more
core  

Primers used in real-time polymerase chain reaction quantification and reverse transcription-polymerase chain reaction.

, 2013
Primers used in real-time polymerase chain reaction quantification and reverse transcription-polymerase chain reaction.
Lin Feng (84785), Yuwan Dong (500622), Lili Wang (104605), Fukui Feng (500621), Lingling E (500620), Dongsheng Wang (162509), Hao Wu (65943), Hongchen Liu (500623)   +7 more
core   +1 more source

OVERVIEW OF METHODS AND POLYMERASE-CHAIN REACTION MODELS

Medična Informatika ta Inženerìâ, 2015
In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques ...
A. S. Sverstyuk, T. V. Bihunyak, B. O. Pereviznyk   +2 more
doaj   +1 more source