Results 81 to 90 of about 881,637 (262)

Detecting beef meatball contamination with polymerase chain reaction [PDF]

open access: yesBiotika, 2017
The objective of the study was to describe how much rat and swine primer developed from cytochrome b could detect rat and pork in processed beef products sold in North Maluku.
Hoda A., Sapsuha Y., Syafie Y.
doaj  

OVERVIEW OF METHODS AND POLYMERASE-CHAIN REACTION MODELS

open access: yesMedična Informatika ta Inženerìâ, 2015
In the article overviewed the methods and polymerase chain reaction models for the mathematical modeling and evaluating minimum time required problem for of each stage of the cycle and showed how to increase the studied molecular biology techniques ...
A. S. Sverstyuk   +2 more
doaj   +1 more source

Effects of IGFBP4 deficiency on human preadipocyte proliferation and differentiation through the IGF1R/AKT pathway

open access: yesFEBS Open Bio, EarlyView.
IGFBP4 knockdown (KD) impairs preadipocyte proliferation and is associated with IGF1R protein downregulation and attenuated AKT phosphorylation. The mechanisms by which IGFBP4 KD influences the IGF1R/AKT signaling pathway involve newly synthesized proteins and lysosomal degradation pathways. Created in BioRender.
Yujia Guo   +6 more
wiley   +1 more source

Design of an Improved Polymerase Chain Reaction (PCR) Assay for Molecular Detection of Streptococcus pneumonia

open access: yesMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Qum, 2013
Background and Objectives: Streptococcus pneumoniae accounts for bacterial meningitis and is an important cause of morbidity among children and elderly. Control of this disease depends on rapid detection of the causative bacteria.
Fahimeh Aghamiri   +3 more
doaj   +2 more sources

Polymerase chain reaction-based gene removal from plasmids

open access: yesData in Brief, 2015
This data article contains supplementary figures and methods to the research article entitled, “Multiplex gene removal by two-step polymerase chain reactions” (Krishnamurthy et al., Anal.
Vishnu Vardhan Krishnamurthy   +4 more
doaj   +1 more source

Ionomycin suppresses cancer cell growth by disrupting mitochondrial transcription

open access: yesFEBS Open Bio, EarlyView.
In this study, we identify a new function for the selective Ca2+ ionophore, ionomycin, as an inhibitor of mitochondrial transcription. Both total and nascent RNA analyses revealed that ionomycin treatment reduces the transcription of mitochondrial genes.
Lishen Wang   +10 more
wiley   +1 more source

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

open access: yesFEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks   +4 more
wiley   +1 more source

Evaluating the effect of γ‐oryzanol on MASLD pathology using a medaka fish model

open access: yesFEBS Open Bio, EarlyView.
This study explores a liver disease called MASLD, which is increasing worldwide and can lead to serious damage. Researchers used medaka fish instead of rodents to test a food compound, γ‐oryzanol. Fish fed this compound had less liver fat and healthier gut bacteria.
Yukako Ito   +7 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

open access: yesFEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

DIAGNOSIS OF CANINE TRYPANOSOMOSIS BY POLYMERASE CHAIN REACTION [PDF]

open access: yesJournal of Veterinary and Animal Sciences, 2013
A clinical case of trypanosomosis in a German Shepherd dog and its diagnosis by polymerase chain reaction(PCR) was described.The dog was presented with clinical signs such as pyrexia, bilateral corneal opacity, pale mucous membranes, emaciation and ...
P.V.Tresamol   +4 more
doaj  

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