Results 261 to 270 of about 1,450,683 (312)

Viral Polymerase Chain Reaction Testing of the Cerebrospinal Fluid: Comprehensive Care for Neonates With a Fever. [PDF]

Cureus
Levy A, Ross A, Delle Donne A.
europepmc   +1 more source

Comment on: “Atom‐Modified gDNA Enhances Cleavage Activity of TtAgo Enabling Ultrasensitive Nucleic Acid Testing”

Advanced Science, Volume 12, Issue 13, April 3, 2025.
Abstract The recent article by Zhang et al. piqued the interest. An atom‐modification‐based strategy is reported to enhance the cleavage activity of TtAgo, improving its practicability in TtAgo‐based nucleic acid testing. Specifically, the 2′‐fluorine (2′F)‐modified guide DNA (2′F‐gDNA) shows significant enhancement in the cleavage activity of TtAgo on
Yue Tang, Xiao‐han Wang, Xu Xu, Lu‐sheng Xin, Xiu‐dan Wang, Xin‐min Li   +5 more
wiley   +1 more source

Exploration of different quantitative polymerase chain reaction-based genotyping methods to distinguish Apcmin/+ mice from wildtype mice. [PDF]

PLoS One
Sun Y, Zhou T, Ye S, Lau EYT, Zeng Y, Liang JQ.   +5 more
europepmc   +1 more source

Multi‐Omics Analysis Reveals Impacts of LincRNA Deletion on Yeast Protein Synthesis

Advanced Science, Volume 12, Issue 13, April 3, 2025.
This study demonstrates that the deletion of specific lincRNAs in yeast affects protein secretion. An engineered yeast strain, with multiple genetic modifications including lincRNA deletion, achieves a high yield of 4.1 g L⁻1 in fed‐batch cultivation.
Ling Qin, Yuyang Pan, Songlyu Xue, Zhibo Yan, Chufan Xiao, Xiufang Liu, Dan Yuan, Jin Hou, Mingtao Huang   +8 more
wiley   +1 more source

Bioprinted Patient‐Derived Organoid Arrays Capture Intrinsic and Extrinsic Tumor Features for Advanced Personalized Medicine

Advanced Science, EarlyView.
An embedded bioprinting enabled‐arrayed patient‐derived organoids (Eba‐PDO) platform that replicates intrinsic and extrinsic tumor characteristics is introduced. Eba‐PDOs more accurately mimic tissue than standard PDOs (Std‐PDOs) due to maturation in the tumor microenvironment.
Jonghyeuk Han, Hye‐Jin Jeong, Jeonghan Choi, Hyeonseo Kim, Taejoon Kwon, Kyungjae Myung, Kyemyung Park, Jung In Park, Samuel Sánchez, Deok‐Beom Jung, Chang Sik Yu, In Ho Song, Jin‐Hyung Shim, Seung‐Jae Myung, Hyun‐Wook Kang, Tae‐Eun Park   +15 more
wiley   +1 more source

The Standardization and Quantification of Nuclear Factor Kappa B p65 by Real-Time Quantitative Polymerase Chain Reaction. [PDF]

Cureus
Gnanaskandan S, Karunakaran U, Srikanth P.   +2 more
europepmc   +1 more source

Iron Deficiency Impairs Dendritic Cell Development and Function, Compromising Host Anti‐Infection Capacity

Advanced Science, EarlyView.
Iron deficiency reduces monocyte dendritic cell progenitor (MDP) numbers by inhibiting migration and proliferation while promoting apoptosis, subsequently reducing downstream common dendritic cell precursors (CDP) and dendritic cell (DC). Impaired DCs enter the lungs via blood circulation, where reduced antigen presentation impairs T‐cell activation ...
Quanzhong Ren, Xiaotong Xu, Zheng Dong, Jiahuang Qiu, Qing'e Shan, Rui Chen, Yajun Liu, Juan Ma, Sijin Liu   +8 more
wiley   +1 more source

Discrepancies in the Diagnosis of Congenital <i>Toxoplasma gondii</i> Infection Between B1 Gene Semi-Nested Polymerase Chain Reaction and Serological Analyses. [PDF]

Microorganisms
Uchida A, Tanimura K, Shirai K, Ashina M, Fujioka K, Morioka I, Sasai M, Yamamoto M, Yamada H.   +8 more
europepmc   +1 more source

PCSK9 Promotes the Malignancy of Triple‐negative Breast Cancer Cells by Reducing Cholesterol Levels at the Plasma Membrane to Activate EGFR and HER3

Advanced Science, EarlyView.
By decreasing cholesterol and lipid raft levels in the plasma membrane, proprotein convertase subtilisin/kexin type 9 (PCSK9) boosts human epidermal growth factor receptor 1 and 3 (EGFR and HER3) activation, driving tumor growth and metastasis in triple‐negative breast cancer (TNBC).
Tianhong Li, Renfei Wu, Kathy Qian Luo
wiley   +1 more source

Comparison of Commercially Available Thermostable DNA Polymerases with Reverse Transcriptase Activity in Coupled Reverse Transcription Polymerase Chain Reaction Assays. [PDF]

Methods Protoc
Smirnova EV, Blagodatskikh KA, Barsova EV, Varlamov DA, Kramarov VM, Ignatov KB.   +5 more
europepmc   +1 more source