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Zirconium-Metalloporphyrin Frameworks-Luminol Competitive Electrochemiluminescence for Ratiometric Detection of Polynucleotide Kinase Activity.

Analytical Chemistry, 2020
We propose a novel competitive mechanism involving the dissolved oxygen (O2) between zirconium-based porphyrinic metal-organic framework nanoparticles (NMOFs) and luminol into a ratiometric electrochemiluminescence (ECL) biosensing interface.
Guangyao Zhang   +5 more
semanticscholar   +1 more source

Polynucleotide analogues

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1966
Abstract Methylation of guanosine 5′-phosphate under suitable conditions gives 1-methylguanylic acid. This was converted into the pyrophosphate which was then polymerised using polynucleotide phosphorylase from Escherichia coli. Some physical properties of poly N 1 - methylguanylic acid and the formation of a stable organised structure ...
F. Pochon, A.M. Michelson
openaire   +10 more sources

Polynucleotides X. Oligonucleotides and their association with polynucleotides

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1967
Abstract Titration studies on a series of oligo (A)'s are described and the thermal stability of the structural form at acid pH has been examined. The stability of complexes formed between oligonucleotides and a complementary polynucleotide has also been studied using homologous series of oligo (A)'s, (C)'s, (I)'s, and (U)'s, and a wide variety of ...
A.M. Michelson, C. Monny
openaire   +3 more sources

TiO2/g-C3N4/CdS Nanocomposite-Based Photoelectrochemical Biosensor for Ultrasensitive Evaluation of T4 Polynucleotide Kinase Activity.

Analytical Chemistry, 2019
Herein, an efficient photoelectrochemical (PEC) platform was constructed by a cosensitization strategy with a cascade energy level arrangement for the ultrasensitive evaluation of T4 polynucleotide kinase (T4 PNK).
Pan-Pan Li   +4 more
semanticscholar   +1 more source

Model for the elongation of polynucleotide chains by polynucleotide phosphorylase

Journal of Molecular Biology, 1970
Abstract The experiments described in this paper were designed to elucidate the mechanism of elongation of a polynucleotide chain catalysed by polynucleotide phosphorylase. The problem has been approached in three different ways, essentially: (1) examination of the size distribution of the end products isolated during the course of the reaction; (2 ...
M. Grunberg-Manago   +2 more
openaire   +3 more sources

Mimic Peroxidase- and Bi2S3 Nanorod-Based Photoelectrochemical Biosensor for Signal-On Detection of Polynucleotide Kinase.

Analytical Chemistry, 2018
We demonstrate for the first time the development of a mimic peroxidase- and bismuth sulfide (Bi2S3) nanorod-based photoelectrochemical (PEC) biosensor for signal-on detection of polynucleotide kinase (PNK) on the basis of manganese-based mimic enzyme ...
Lin Cui   +5 more
semanticscholar   +1 more source

A Dual-Enzyme-Assisted Three-Dimensional DNA Walking Machine Using T4 Polynucleotide Kinase as Activators and Application in Polynucleotide Kinase Assays.

Analytical Chemistry, 2018
T4 polynucleotide kinase (T4 PNK), an intracellular kinase, catalyzes the phosphorylation of 5'-hydroxyl termini in nucleic acids and plays a crucial role in DNA-related physiological activities.
Chang Feng   +6 more
semanticscholar   +1 more source

A modified exponential amplification reaction (EXPAR) with an improved signal-to-noise ratio for ultrasensitive detection of polynucleotide kinase.

Chemical Communications, 2019
We applied a modified exponential amplification reaction (EXPAR) strategy to design a label-free and "one-pot" biosensor for ultrasensitive detection of polynucleotide kinase (PNK).
Yu-Peng Zhang   +5 more
semanticscholar   +1 more source

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