Results 261 to 270 of about 93,488 (303)

The interaction of polynucleotides with cations

Biochimica et Biophysica Acta, 1959
Abstract The interaction with divalent cations of the synthetic polynucleotides, polyadenylic acid and polyuridylic acid, has been studied by conductiometric titration. It is shown that further binding of divalent cation by polynucleotide ceases when one equivalent of cation has been added per mole of polymer phosphate, and that the titration curve ...
Sylvia Huang, Gary Felsenfeld
openaire   +3 more sources

Sensitive detection of polynucleotide kinase using rolling circle amplification-induced chemiluminescence.

Chemical Communications, 2014
We develop a new method for the sensitive detection of polynucleotide kinase (PNK) using rolling circle amplification-induced chemiluminescence. This method exhibits high sensitivity with a detection limit of 2.20 × 10(-4) U mL(-1), which is superior to ...
Wei Tang, Guichi Zhu, Chun-Yang Zhang
semanticscholar   +1 more source

The relationship of mono- and polynucleotide conformation to catalysis by polynucleotide phosphorylase

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1970
Abstract 8-Bromoguanosine 5′-diphosphate, 8-oxoguanosine 5′-diphosphate, 6-methylcytidine 5′-diphosphate and 2,6-quinazoline dione 1′-ribosyl 5′-diphosphate are inactive as substrates for homopolymer synthesis with polynucleotide phosphorylase. They inhibit polymerization and exchange but not phosphorolysis.
A.M. Michelson, C. Monny, A.M. Kapuler
openaire   +3 more sources

Detection of polynucleotide kinase activity by using a gold electrode modified with magnetic microspheres coated with titanium dioxide nanoparticles and a DNA dendrimer.

In Analysis, 2014
In this paper, we have designed a signal amplified method for the electrochemical determination of polynucleotide kinase activity. It is based on (a) the peroxidase-like activity of magnetite microspheres (MNPs), (b) the specific recognition capabilities
Guangfeng Wang, Ling Chen, Xiu-Juan He, Yanhong Zhu, Xiaojun Zhang   +4 more
semanticscholar   +1 more source

5'-end labeling of RNA with [γ-32P]ATP and T4 polynucleotide kinase.

Cold Spring Harbor Protocols, 2014
This protocol uses T4 polynucleotide kinase to catalyze the transfer of a radiolabeled, terminal (γ) phosphate of ATP to the 5'-hydroxyl terminus of a DNA or RNA molecule.
D. Rio
semanticscholar   +1 more source

Sensitive nanochannel biosensor for T4 polynucleotide kinase activity and inhibition detection.

Analytical Chemistry, 2013
5'-Polynucleotide kinase is a crucial class of enzyme that catalyzes the phosphorylation of nucleic acids with 5'-hydroxyl termini. This process regulates many important cellular events, especially DNA repair during strand damage and interruption.
Lei Lin, Yang Liu, Jing Yan, Xingsheng Wang, Jinghong Li   +4 more
semanticscholar   +1 more source

A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition.

Journal of materials chemistry. B, 2013
A novel fluorescence polarization (FP) nanosensor based on λ exonuclease cleavage reaction and FP enhancement effect of gold nanoparticles (AuNPs) has been developed for the detection of T4 polynucleotide kinase activity and inhibition.
Yong Huang, Jia Chen, Ming Shi, Shulin Zhao, Zheng-Feng Chen, H. Liang   +5 more
semanticscholar   +1 more source

Chemical Synthesis of Polynucleotides

Angewandte Chemie International Edition in English, 1972
AbstractCurrent methodology for the chemical synthesis of short chains (up to about twenty nucleotide units) of deoxyribopolynucleotides is reviewed.
P. J. Cashion, H G Khorana, A. Yamazaki, K. L. Agarwal   +3 more
openaire   +3 more sources

Label free fluorescence turn-on detection of polynucleotide kinase activity with a perylene probe.

Chemical Communications, 2012
A single stranded oligonucleotide could induce aggregation of a perylene probe, the probe's monomer fluorescence was efficiently quenched. However, when the oligonucleotide was 5'-phosphorylated by polynucleotide kinase, it could be very efficiently ...
Huping Jiao, Bin Wang, Jian Chen, Dongli Liao, Wenying Li, Cong Yu   +5 more
semanticscholar   +1 more source

[32] Interferon induction by polynucleotides, modified polynucleotides, and polycarboxylates

1981
Publisher Summary This chapter discusses the procedures for obtaining high interferon yields in primary rabbit kidney (PRK) and human fibroblasts (HF) cells, in response to (I) n . (C) n . The chapter discusses several procedures that are developed to enhance the interferon response of PRK and HF cell cultures to (I) n . (C) n .
openaire   +3 more sources