Results 21 to 30 of about 93,488 (303)
Biosensors, 2023 Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively.
Jingyi Si +7 more
doaj +1 more source
Binding of phenazinium dye safranin T to polyriboadenylic acid: spectroscopic and thermodynamic study. [PDF]
PLoS ONE, 2014 Here, we report results from experiments designed to explore the association of the phenazinium dye safranin T (ST, 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride) with single and double stranded form of polyriboadenylic acid (hereafter poly-A ...
Ankur Bikash Pradhan +3 more
doaj +1 more source
An A-form of poly[d(A-C)] · poly[d(G-T)] induced by mercury (II) as studied by UV and FTIR spectroscopies [PDF]
, 1995 The conformational changes of poly[d(A-C)]·poly[d(GT)] induced by Hg(CiO4)2 in aqueous solution have been studied using UV absorption and fourth derivative spectrophotometries, and FTIR spectroscopy.
Garcia-Quintana, David +2 more
core +1 more source
When DNA Polymerases Multitask: Functions Beyond Nucleotidyl Transfer
Frontiers in Molecular Biosciences, 2022 DNA polymerases catalyze nucleotidyl transfer, the central reaction in synthesis of DNA polynucleotide chains. They function not only in DNA replication, but also in diverse aspects of DNA repair and recombination.
Denisse Carvajal-Maldonado +3 more
doaj +1 more source
Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase
Journal of Biological Chemistry, 1975 The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y Chou, P McPhie, M F Singer
openaire +2 more sources
A Raman spectroscopic study of the interaction between nucleotides and the DNA binding protein gp32 of bacteriophage T4 [PDF]
, 1987 Raman spectra of the bacteriophage T4 denaturing protein gp32, its complex with the polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA)8 and (dA)2, were recorded and interpreted.
Greve, J., Mul, F.F.M. de, Otto, C.
core +2 more sources
Molecules, 2012 We report herein the synthesis and characterization of 9-(2-diallylaminoethyl) adenine. We evaluated two different synthetic routes starting with adenine where the optimal route was achieved through coupling of 9-(2-chloroethyl)adenine with diallylamine.
Karyn Usher +4 more
doaj +1 more source
Analytical Chemistry, 2017 5'-Polynucleotide kinase such as T4 polynucleotide kinase (T4 PNK) may catalyze the phosphorylation of 5'-hydroxyl termini in nucleic acids, playing a crucial role in DNA replication, DNA recombination, and DNA damage repair. Here, we demonstrate for the
Li-juan Wang +3 more
semanticscholar +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
core +1 more source
Synthesis of Polynucleotide Analogs Containing a Polyvinyl Alcohol Backbone
Molecules, 2008 Water soluble homo-base polynucleotide analogues were synthesized in whichpolyvinyl alcohol and partially phosphonated polyvinyl alcohol constituted the backbones,onto which were grafted uracil or adenine via 1,3-dioxane spacers formed by acetalformation
Per Carlsen, Qiang Yu
doaj +1 more source