Results 21 to 30 of about 95,296 (292)

The dynamics of polynucleotide chain consisting of two different homogeneous sequences, divided by interface [PDF]

Компьютерные исследования и моделирование, 2013
To research dynamics of inhomogeneous polynucleotide DNA chain the Y-model with no dissipation term was used. Basing on this model using numerical methods calculations were carried out, which have shown the behaviour of nonlinear conformational ...
Andrei Anatolevich Grinevich, Artem Andreevich Ryasik, L. V. Yakushevich   +2 more
doaj   +1 more source

Polynucleotide phosphorylase protects against renal tubular injury via blocking mt-dsRNA-PKR-eIF2α axis

Nature Communications, 2023
Renal tubular atrophy is a hallmark of chronic kidney disease. The study by Zhu et al. reveals the protective role of tubular polynucleotide phosphorylase (PNPT1) against renal atrophy by blocking the leakage of mitochondrial dsRNAs into cytoplasm where ...
Yujie Zhu, Mingchao Zhang, Weiran Wang, Shuang Qu, Minghui Liu, Weiwei Rong, Wenwen Yang, Hongwei Liang, Caihong Zeng, Xiaodong Zhu, Limin Li, Zhihong Liu, Ke Zen   +12 more
doaj   +1 more source

Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds

Journal of Functional Biomaterials, 2012
Biopolymers are easily denatured by heating, a change in pH or chemical substances when they are immobilized on a substrate. To prevent denaturation of biopolymers, we developed a method to trap a polynucleotide on a substrate by hydrogen bonding using ...
R. Maeda, T. Isoda
doaj   +1 more source

Binding to the minor groove of the double-strand, tau protein prevents DNA from damage by peroxidation. [PDF]

PLoS ONE, 2008
Tau, an important microtubule associated protein, has been found to bind to DNA, and to be localized in the nuclei of both neurons and some non-neuronal cells.
Yan Wei, Mei-Hua Qu, Xing-Sheng Wang, Lan Chen, Dong-Liang Wang, Ying Liu, Qian Hua, Rong-Qiao He   +7 more
doaj   +1 more source

Impact of PNKP mutations associated with microcephaly, seizures and developmental delay on enzyme activity and DNA strand break repair [PDF]

, 2012
Microcephaly with early-onset, intractable seizures and developmental delay (MCSZ) is a hereditary disease caused by mutations in polynucleotide kinase/phosphatase (PNKP), a DNA strand break repair protein with DNA 5'-kinase and DNA 3'-phosphatase ...
Alexandra K. Walker, Beckman, Bernstein, Breslin, Breslin, Brodsky, Buck, Buck, Caldecott, Caldecott, Chappell, Christopher A. Walsh, Date, Dingwall, Dobson, Edward C. Gilmore, El-Khamisy, Evans, Garces, Hsiang, Interthal, John J. Reynolds, Karimi-Busheri, Keith W. Caldecott, Koch, Kouzminova, Kuzminov, Lee, Lindahl, Loizou, McCullough, Moreira, Riballo, Segal-Raz, Shen, Sprules, Takashima, Wang, Wang, Ward, Ward, Whitehouse   +41 more
core   +1 more source

The Effect of Divalent Metal Ions on the Temperature Stability of Poly(I:C) Duplex

Journal of Spectroscopy, 2020
The effect of divalent ions on the thermal stability of the double-stranded polynucleotide polyinosinic:polycytidylic acid (poly(I:C)) in a buffer solution (pH 7) was studied by UV absorption spectroscopy and thermal denaturation by measuring the ...
Evgeniya Usenko, Vladimir Valeev, Alexander Glamazda, Victor Karachevtsev   +3 more
doaj   +1 more source

Binding of phenazinium dye safranin T to polyriboadenylic acid: spectroscopic and thermodynamic study. [PDF]

PLoS ONE, 2014
Here, we report results from experiments designed to explore the association of the phenazinium dye safranin T (ST, 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride) with single and double stranded form of polyriboadenylic acid (hereafter poly-A ...
Ankur Bikash Pradhan, Lucy Haque, Snigdha Roy, Suman Das   +3 more
doaj   +1 more source

Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]

, 2016
Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen, Braeckmans, Kevin, Remaut, Katrien   +2 more
core   +1 more source

Nonlinear waves in the DNA molecules containing a boundary between two homogeneous regions [PDF]

Компьютерные исследования и моделирование, 2009
Propagation of nonlinear conformational waves through the boundary dividing the double polynucleotide chain into two different homogeneous regions is investigated.
L. V. Yakushevich, A. V. Savin, L. I. Manevitch   +2 more
doaj   +1 more source

Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription

Biosensors, 2023
Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively.
Jingyi Si, Wei Zhou, Ying Fang, Da Zhou, Yifan Gao, Qunyan Yao, Xizhong Shen, Changfeng Zhu   +7 more
doaj   +1 more source