Results 21 to 30 of about 93,488 (303)

Label-Free Detection of T4 Polynucleotide Kinase Activity and Inhibition via Malachite Green Aptamer Generated from Ligation-Triggered Transcription

open access: yesBiosensors, 2023
Polynucleotide kinase (PNK) is a key enzyme that is necessary for ligation-based DNA repair. The activity assay and inhibitor screening for PNK may contribute to the prediction and improvement of tumor treatment sensitivity, respectively.
Jingyi Si   +7 more
doaj   +1 more source

Binding of phenazinium dye safranin T to polyriboadenylic acid: spectroscopic and thermodynamic study. [PDF]

open access: yesPLoS ONE, 2014
Here, we report results from experiments designed to explore the association of the phenazinium dye safranin T (ST, 3,7-diamino-2,8-dimethyl-5-phenylphenazinium chloride) with single and double stranded form of polyriboadenylic acid (hereafter poly-A ...
Ankur Bikash Pradhan   +3 more
doaj   +1 more source

An A-form of poly[d(A-C)] · poly[d(G-T)] induced by mercury (II) as studied by UV and FTIR spectroscopies [PDF]

open access: yes, 1995
The conformational changes of poly[d(A-C)]·poly[d(GT)] induced by Hg(CiO4)2 in aqueous solution have been studied using UV absorption and fourth derivative spectrophotometries, and FTIR spectroscopy.
Garcia-Quintana, David   +2 more
core   +1 more source

When DNA Polymerases Multitask: Functions Beyond Nucleotidyl Transfer

open access: yesFrontiers in Molecular Biosciences, 2022
DNA polymerases catalyze nucleotidyl transfer, the central reaction in synthesis of DNA polynucleotide chains. They function not only in DNA replication, but also in diverse aspects of DNA repair and recombination.
Denisse Carvajal-Maldonado   +3 more
doaj   +1 more source

Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase

open access: yesJournal of Biological Chemistry, 1975
The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y Chou, P McPhie, M F Singer
openaire   +2 more sources

A Raman spectroscopic study of the interaction between nucleotides and the DNA binding protein gp32 of bacteriophage T4 [PDF]

open access: yes, 1987
Raman spectra of the bacteriophage T4 denaturing protein gp32, its complex with the polynucleotides poly(rA), poly(dA), poly(dT), poly(rU), and poly(rC), and with the oligonucleotides (dA)8 and (dA)2, were recorded and interpreted.
Greve, J., Mul, F.F.M. de, Otto, C.
core   +2 more sources

Synthesis, Cyclopolymerization and Cyclo-Copolymerization of 9-(2-Diallylaminoethyl)adenine and Its Hydrochloride Salt

open access: yesMolecules, 2012
We report herein the synthesis and characterization of 9-(2-diallylaminoethyl) adenine. We evaluated two different synthetic routes starting with adenine where the optimal route was achieved through coupling of 9-(2-chloroethyl)adenine with diallylamine.
Karyn Usher   +4 more
doaj   +1 more source

Single-Molecule Detection of Polynucleotide Kinase Based on Phosphorylation-Directed Recovery of Fluorescence Quenched by Au Nanoparticles.

open access: yesAnalytical Chemistry, 2017
5'-Polynucleotide kinase such as T4 polynucleotide kinase (T4 PNK) may catalyze the phosphorylation of 5'-hydroxyl termini in nucleic acids, playing a crucial role in DNA replication, DNA recombination, and DNA damage repair. Here, we demonstrate for the
Li-juan Wang   +3 more
semanticscholar   +1 more source

Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]

open access: yes, 2016
Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen   +2 more
core   +1 more source

Synthesis of Polynucleotide Analogs Containing a Polyvinyl Alcohol Backbone

open access: yesMolecules, 2008
Water soluble homo-base polynucleotide analogues were synthesized in whichpolyvinyl alcohol and partially phosphonated polyvinyl alcohol constituted the backbones,onto which were grafted uracil or adenine via 1,3-dioxane spacers formed by acetalformation
Per Carlsen, Qiang Yu
doaj   +1 more source

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