Results 41 to 50 of about 74,970 (276)
Kinetic studies on the phosphorolysis of polynucleotides by polynucleotide phosphorylase
Journal of Biological Chemistry, 1975 The kinetics of the phosphorolysis of polynucleotide (as differentiated from oligonucleotide) by polynucleotide phosphorylase of Micrococcus luteus has been investigated. Double reciprocal plots of initial velocity against either inorganic phosphate or polynucleotide concentration are linear, and furthermore, the affinity of the enzyme for either ...
J Y Chou, P McPhie, M F Singer
openaire +2 more sources
Driven translocation of a polynucleotide chain through a nanopore--A continuous time Monte-Carlo study [PDF]
Physical Review E74, 011911 (2006), 2006 Using continuous time Monte-Carlo method we simulated the translocation of a polynucleotide chain driven through a nanopore by an electric field. We have used two models of driven diffusion due to the electric field. The chain may have strong interaction with the pore, and depends on which end of the chain first enters the pore.
arxiv +1 more source
Translocation of structured polynucleotides through nanopores [PDF]
Physical Biology 1 (2004) 19-26, 2003 We investigate theoretically the translocation of structured RNA/DNA molecules through narrow pores which allow single but not double strands to pass. The unzipping of basepaired regions within the molecules presents significant kinetic barriers for the translocation process.
arxiv +1 more source
Molecular dynamics study on the mechanism of polynucleotide encapsulation by chitosan
Scientific Reports, 2017 The safe and effective delivery of therapeutic genes into target cell interiors is of great importance in gene therapy. Chitosan has been extensively studied as a gene delivery carrier due to its good biocompatibility and biodegradability.
Jia-Wei Shen +5 more
doaj +1 more source
Synthesis of Polynucleotide Analogs Containing a Polyvinyl Alcohol Backbone
Molecules, 2008 Water soluble homo-base polynucleotide analogues were synthesized in whichpolyvinyl alcohol and partially phosphonated polyvinyl alcohol constituted the backbones,onto which were grafted uracil or adenine via 1,3-dioxane spacers formed by acetalformation
Per Carlsen, Qiang Yu
doaj +1 more source
Fluorescent labeling of plasmid DNA and mRNA : gains and losses of current labeling strategies [PDF]
, 2016 Live-cell imaging has provided the life sciences with insights into the cell biology and dynamics. Fluorescent labeling of target molecules proves to be indispensable in this regard. In this Review, we focus on the current fluorescent labeling strategies
Rombouts, Koen +2 more
core +1 more source
Stochastic sensing of polynucleotides using patterned nanopores [PDF]
Phys. Rev. X 2, 021002 (2012), 2012 The effect of the microscopic structure of a pore on polymer translocation is studied using Langevin dynamics simulation, and the consequence of introducing patterned stickiness inside the pore is investigated. It is found that the translocation process is extremely sensitive to the detailed structure of such patterns with faster than exponential ...
arxiv +1 more source
Molecules, 2012 We report herein the synthesis and characterization of 9-(2-diallylaminoethyl) adenine. We evaluated two different synthetic routes starting with adenine where the optimal route was achieved through coupling of 9-(2-chloroethyl)adenine with diallylamine.
Karyn Usher +4 more
doaj +1 more source
Impact of PNKP mutations associated with microcephaly, seizures and developmental delay on enzyme activity and DNA strand break repair [PDF]
, 2012 Microcephaly with early-onset, intractable seizures and developmental delay (MCSZ) is a hereditary disease caused by mutations in polynucleotide kinase/phosphatase (PNKP), a DNA strand break repair protein with DNA 5'-kinase and DNA 3'-phosphatase ...
Alexandra K. Walker +41 more
core +1 more source
Clinical symptoms and molecular biology research in a family with oculopharyngeal muscular dystrophy
Chinese Journal of Contemporary Neurology and Neurosurgery, 2021 Objective To investigate the clinical symptoms and molecular biological characteristics in a family with oculopharyngeal muscular dystrophy (OPMD). Methods Clinical data of the family members were collected.
HUANG Kai +6 more
doaj +1 more source