Results 141 to 150 of about 76,419 (179)
The activity of mammalian polynucleotide ligase on X-irradiated DNAs
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978 Selected samples of heterogeneous DNA from calf thymus with similar number-average molecular weight, Mn, and a low incidence of single-strand breaks were exposed in aqueous solutions to a mild X-ray dose of 1500 rads. The irradiation produced on the average about 0.2 bihelical and 2.2 monohelical scissions per DNA molecule of 1708 000 Mn.
M. Mathelet +3 more
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DNA replication by phage T4 rII mutants without polynucleotide ligase (gene 30)
Biochemical and Biophysical Research Communications, 1969 Abstract In phage T4-infected E. coli , the polynucleotide ligase coded for by T4 gene 30 is not required for DNA replication and phage production when the infecting phage is an rII mutant. The wild-type T4 rII gene product may be a nuclease or control a nuclease activity.
J. D. Karam
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Polynucleotide Ligase and φX174 Single Strand Synthesis [PDF]
Nature New Biology, 1971 A DNA ligase mutant of E. coli when infected with φX174 produces linear single strands which appear in an intracellular pool and in phage particles. The linear single strands, which are infectious in a spheroplast assay, seem to be a normal intermediate in progeny DNA synthesis.
Dan S. Ray +2 more
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Effect of ATP analogues on T4 polynucleotide ligase
Biochemical and Biophysical Research Communications, 1978Abstract The influence of three ATP analogues, α, β-methylene adenosine 5′-triphosphate, β, γ-methylene adenosine 5′-triphosphate and β, γ-imidoadenosine 5′-triphosphate on the activity of T 4 polynucleotide ligase has been investigated. Only β, γ-imidoadenosine 5′-triphosphate was active in the joining reaction; the rate of catalysis being slightly
A.J. Raae, K. Kleppe
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A biological assay for polynucleotide ligase: Recovery of marker activity in DNA-transformation
Biochemical and Biophysical Research Communications, 1967 Ekkehard K.F. Bautz
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Repair of single strand breaks in transforming DNA by polynucleotide ligase
Biochemical and Biophysical Research Communications, 1968 Jun Takagi +2 more
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A new method of assay for polynucleotide ligase
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974Abstract A new method for the assay of polynucleotide ligase is described. It consists of two parallel DNA polymerase reactions with poly(dA) · oligo(dT) as template-primer and d[3H] TTP as precursor; ligase is added to both, but one is supplemented with NMN and the other with NAD.
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Purification and properties of a polynucleotide ligase from calf thymus glands
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract Polynucleotide ligase has been purified over 390-fold from extracts of calf thymus glands. A solid-state model of DNA containing single-strand breaks, namely, the poly(dA) · [poly([ 3 H]dT)-poly(dT)]-cellulose, was used as substrate for the enzymatic assays.
BERTAZZONI, Umberto +2 more
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Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4
Virology, 1970Abstract An intergenic suppressor ( m ) of the amber ligase mutation ( amH 39 x ) was isolated and a number of possible mechanisms of suppression were investigated. The intergenic suppressor does not appear to involve suppression of the amber codon.
V.L. Chan, K. Ebisuzaki, S. Shugar
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Polynucleotide ligase from rat liver after partial hepatectomy
Biochemical and Biophysical Research Communications, 1971Abstract Polynucleotide ligase, which catalyzes the covalent joining of two segments of an interrupted strand in a DNA duplex, was extracted from rat liver. The activity is located in both nuclei and soluble fractions. Its optimal pH was 8.0. The enzyme requires ATP as a cofactor. Its activity was completely dependent on the presence of Mg 2+ .
Machiko Ichimura, Kinji Tsukada
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