Results 141 to 150 of about 3,904 (190)

Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4

open access: closedVirology, 1970
Abstract An intergenic suppressor ( m ) of the amber ligase mutation ( amH 39 x ) was isolated and a number of possible mechanisms of suppression were investigated. The intergenic suppressor does not appear to involve suppression of the amber codon.
Vivien Chan   +2 more
openalex   +3 more sources

A new method of assay for polynucleotide ligase

open access: closedBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974
Abstract A new method for the assay of polynucleotide ligase is described. It consists of two parallel DNA polymerase reactions with poly(dA) · oligo(dT) as template-primer and d[3H] TTP as precursor; ligase is added to both, but one is supplemented with NMN and the other with NAD.
John D. Karkas
openalex   +3 more sources

DNA replication by phage T4 rII mutants without polynucleotide ligase (gene 30)

open access: closedBiochemical and Biophysical Research Communications, 1969
Abstract In phage T4-infected E. coli , the polynucleotide ligase coded for by T4 gene 30 is not required for DNA replication and phage production when the infecting phage is an rII mutant. The wild-type T4 rII gene product may be a nuclease or control a nuclease activity.
J. D. Karam
openalex   +3 more sources

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