Results 131 to 140 of about 19,008 (171)
Some of the next articles are maybe not open access.
Effect of ATP analogues on T4 polynucleotide ligase
Biochemical and Biophysical Research Communications, 1978Abstract The influence of three ATP analogues, α, β-methylene adenosine 5′-triphosphate, β, γ-methylene adenosine 5′-triphosphate and β, γ-imidoadenosine 5′-triphosphate on the activity of T 4 polynucleotide ligase has been investigated. Only β, γ-imidoadenosine 5′-triphosphate was active in the joining reaction; the rate of catalysis being slightly
A J, Raae, K, Kleppe
openaire +2 more sources
Polynucleotide Ligase and φX174 Single Strand Synthesis
Nature New Biology, 1971A DNA ligase mutant of E. coli when infected with φX174 produces linear single strands which appear in an intracellular pool and in phage particles. The linear single strands, which are infectious in a spheroplast assay, seem to be a normal intermediate in progeny DNA synthesis.
R W, Schekman, D S, Ray
openaire +2 more sources
The activity of mammalian polynucleotide ligase on X-irradiated DNAs
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978Selected samples of heterogeneous DNA from calf thymus with similar number-average molecular weight, Mn, and a low incidence of single-strand breaks were exposed in aqueous solutions to a mild X-ray dose of 1500 rads. The irradiation produced on the average about 0.2 bihelical and 2.2 monohelical scissions per DNA molecule of 1708 000 Mn.
M, Mathelet +3 more
openaire +2 more sources
Induction of polynucleotide ligase in human lymphocytes stimulated by phytohemoagglutinin
Biochemical and Biophysical Research Communications, 1972Abstract The treatment of human lymphocytes with phytohemoagglutinin causes the appearance of the activity of polynucleotide ligase, rising at least 50 fold from levels below the background. This increase takes place in the fourth or fifth day after treatment, and is delayed by one day approximately with respect to the rise of DNA synthesis rate; the
A M, Pedrini +4 more
openaire +2 more sources
A new method of assay for polynucleotide ligase
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1974Abstract A new method for the assay of polynucleotide ligase is described. It consists of two parallel DNA polymerase reactions with poly(dA) · oligo(dT) as template-primer and d[3H] TTP as precursor; ligase is added to both, but one is supplemented with NMN and the other with NAD.
openaire +2 more sources
Polynucleotide ligase from rat liver after partial hepatectomy
Biochemical and Biophysical Research Communications, 1971Abstract Polynucleotide ligase, which catalyzes the covalent joining of two segments of an interrupted strand in a DNA duplex, was extracted from rat liver. The activity is located in both nuclei and soluble fractions. Its optimal pH was 8.0. The enzyme requires ATP as a cofactor. Its activity was completely dependent on the presence of Mg 2+ .
K, Tsukada, M, Ichimura
openaire +2 more sources
Purification and properties of a polynucleotide ligase from calf thymus glands
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract Polynucleotide ligase has been purified over 390-fold from extracts of calf thymus glands. A solid-state model of DNA containing single-strand breaks, namely, the poly(dA) · [poly([ 3 H]dT)-poly(dT)]-cellulose, was used as substrate for the enzymatic assays.
BERTAZZONI, Umberto +2 more
openaire +3 more sources
Intergenic suppression of amber polynucleotide ligase mutation in bacteriophage T4
Virology, 1970Abstract An intergenic suppressor ( m ) of the amber ligase mutation ( amH 39 x ) was isolated and a number of possible mechanisms of suppression were investigated. The intergenic suppressor does not appear to involve suppression of the amber codon.
V L, Chan, S, Shugar, K, Ebisuzaki
openaire +2 more sources
The polynucleotide ligase and RNA capping enzyme superfamily of covalent nucleotidyltransferases
Current Opinion in Structural Biology, 2004ATP- and NAD(+)-dependent DNA ligases, ATP-dependent RNA ligases and GTP-dependent mRNA capping enzymes comprise a superfamily of proteins that catalyze nucleotidyl transfer to polynucleotide 5' ends via covalent enzyme-(lysyl-N)-NMP intermediates. The superfamily is defined by five peptide motifs that line the nucleotide-binding pocket and contribute ...
Stewart, Shuman, Christopher D, Lima
openaire +2 more sources
Polynucleotide ligase in mouse cells infected by polyoma virus
Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972Abstract 1. The properties of polynucleotide ligase from mouse cells infected with polyoma virus, and from uninfected mouse cells, were studied in order to investigate the possible involvement of ligase in polyoma DNA replication. 2. Two procedures for the assay of polynucleotide ligase were used to detect ligase in extracts of mouse embryo cells ...
openaire +3 more sources